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[173189_at]

Description AV185394_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV185394 /DEF=Caenorhabditis elegans cDNA clone:yk677g10 : 3prime end, single read.
Cluster 6grp_total > 90      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 14
(-1.9)
A 		4.4
(-3)
A 		4.6
(-3)
A 		58.5
(3)
A 		54
B 1.1
(-3)
A 		3.2
(-2.3)
A 		1.4
(-2.9)
A 		19.8
(3)
A 		19
C 1.7
(-3)
A 		3.9
(-2.5)
A 		1.9
(-3)
A 		25.7
(3)
A 		24
avr 5.6
(-2.4) 		3.8
(-2.8) 		2.6
(-3) 		34.7
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 3.3
(-1.9)
A 		5
(-0.9)
A 		1.5
(-3)
A 		11.5
(3)
A 		2.1
(-2.6)
A 		1.7
(-2.9)
A 		10
B 3.5
(0.3)
A 		1.3
(-3)
A 		2
(-2)
A 		4.5
(1.8)
A 		5.3
(3)
A 		2.7
(-0.9)
A 		4
C 9.5
(3)
A 		2
(-3)
A 		3.8
(-1.6)
A 		2.1
(-2.9)
A 		4.4
(-1.1)
A 		3.2
(-2)
A 		8
avr 5.4
(2) 		2.8
(-2.3) 		2.4
(-3) 		6
(3) 		3.9
(-0.5) 		2.5
(-2.8) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 5.1
(0.2)
A 		1
(-3)
A 		1.2
(-2.8)
A 		8.6
(3)
A 		8
B 0.4
(-2.8)
A 		0.8
(-2.2)
A 		0.3
(-3)
A 		4.2
(3)
A 		4
C 0.6
(-3)
A 		1.1
(-2.2)
A 		0.7
(-2.8)
A 		4.3
(3)
A 		4
avr 2
(-1.4) 		1
(-2.6) 		0.7
(-3) 		5.7
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 0.6
(-2.3)
A 		1.8
(0.5)
A 		0.3
(-3)
A 		2.9
(3)
A 		0.6
(-2.3)
A 		0.4
(-2.8)
A 		3
B 1.2
(-0.2)
A 		0.4
(-3)
A 		0.5
(-2.6)
A 		1.2
(-0.2)
A 		2.1
(3)
A 		1
(-0.9)
A 		2
C 3.5
(3)
A 		0.6
(-2.6)
A 		1
(-1.8)
A 		0.4
(-3)
A 		1.4
(-1.1)
A 		1.1
(-1.6)
A 		3
avr 1.8
(3) 		0.9
(-1.5) 		0.6
(-3) 		1.5
(1.5) 		1.4
(1) 		0.8
(-2) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173189_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26919
Sequence Type Exemplar sequence
Representative Public ID AV185394 NCBI
Target Description AV185394_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV185394 /DEF=Caenorhabditis elegans cDNA clone:yk677g10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182258(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182258 NCBI Caenorhabditis elegans 2RSSE.1 (2RSSE.1) mRNA, complete cds. 11/11 None
SNAP00000016284 ENSEMBL cdna:SNAP chromosome:CEL140:II:15272455:15273204:1 10/11 None
GENEFINDER00000016287 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15267553:15275579:1 11/11 None
2RSSE.1 ENSEMBL cdna:known chromosome:CEL140:II:15268102:15273204:1 gene:2RSSE.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15270832-15273201(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl 2RSSE.1
Entrez Gene 353411 Entrez gene
SwissProt Q8I133 EMBL-EBI
WormBase CE32785 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD59137 Hypothetical protein 2RSSE.1 [Caenorhabditis elegans] ref|NP_872058.1| 2RSSE.1 [Caenorhabditis elegans] 0.0
blast CAI79118 Hypothetical protein 2RSSE.2 [Caenorhabditis elegans] ref|NP_001021109.1| 2RSSE.2 [Caenorhabditis elegans] 1.0E-114
blast AAN11778 CG32149-PB, isoform B [Drosophila melanogaster] ref|NP_730061.1| CG32149-PB, isoform B [Drosophila melanogaster] 1.0E-59
blast AAF49609 CG32149-PC, isoform C [Drosophila melanogaster] ref|NP_730060.1| CG32149-PC, isoform C [Drosophila melanogaster] 1.0E-59
blast AAN11778 CG32149-PB, isoform B [Drosophila melanogaster] ref|NP_730061.1| CG32149-PB, isoform B [Drosophila melanogaster] 3.0E-59
blast CAD59137 Hypothetical protein 2RSSE.1 [Caenorhabditis elegans] ref|NP_872058.1| 2RSSE.1 [Caenorhabditis elegans] 8.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.4E-23
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.4E-23
Sequence
Target Sequence 
>C. ELEGANS:173189_AT
tgttcacttttcataatccatccaacngtngtgctcgtggcgaagagttctccgcgttgg
cggcaaagtgtgcgggcgcctactcgctggccgccatccacctggcngaagnagcgtcac
cggagcccactccgacaacctcgaagcctccacgtggcaacggcgtcgggcgtgccngga
gtctgaagcagcacgcgttgacccagacgacggatcatccgaagagaagcgtgtcgatcg
cggctaaggatccgtatccaactgat

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTCACTTTTCATAATCCATCCAA 17 563 17 Antisense
GTGCTCGTGGCGAAGAGTTCTCCGC 209 479 47 Antisense
TTCTCCGCGTTGGCGGCAAAGTGTG 415 693 64 Antisense
TGGCGGCAAAGTGTGCGGGCGCCTA 313 543 74 Antisense
GGAGTCTGAAGCAGCACGCGTTGAC 206 517 194 Antisense
AGTCTGAAGCAGCACGCGTTGACCC 15 61 196 Antisense
TGACCCAGACGACGGATCATCCGAA 189 569 215 Antisense
CGGATCATCCGAAGAGAAGCGTGTC 480 241 227 Antisense
AGAAGCGTGTCGATCGCGGCTAAGG 11 73 241 Antisense
GATCGCGGCTAAGGATCCGTATCCA 280 417 252 Antisense
GGCTAAGGATCCGTATCCAACTGAT 285 539 258 Antisense
Sequence Source Affymetrix Proprietary Database