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[173183_s_at]

Description AV181386_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV181386 /DEF=Caenorhabditis elegans cDNA clone:yk619f6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 47      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 77      6grp_total > 45      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 46      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1367
1 2 3 4 range
A 1346.8
(-0.7)
P 		1901.7
(1.8)
P 		2161.9
(3)
P 		853.9
(-3)
P 		1308
B 1814.1
(1.7)
P 		1713.8
(1.2)
P 		2071.9
(3)
P 		886.5
(-3)
P 		1185
C 1495.5
(-0.3)
P 		1654.7
(0.4)
P 		2220.6
(3)
P 		899.7
(-3)
P 		1321
avr 1552.1
(0.2) 		1756.7
(1.1) 		2151.5
(3) 		880
(-3) 		1272

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 379
1 2 3 4 5 6 range
A 1342.5
(3)
P 		1339.5
(2.9)
P 		1340.2
(2.9)
P 		1154.6
(-3)
P 		1190.1
(-1.9)
P 		1226.6
(-0.7)
P 		188
B 1398.7
(0.8)
P 		1251.2
(-3)
P 		1484
(3)
P 		1286.4
(-2.1)
P 		1323.7
(-1.1)
P 		1421.5
(1.4)
P 		233
C 1470.5
(1.8)
P 		1291.9
(-1.4)
P 		1355.6
(-0.2)
P 		1333.4
(-0.7)
P 		1204.8
(-3)
P 		1533.6
(3)
P 		329
avr 1403.9
(3) 		1294.2
(-1) 		1393.3
(2.6) 		1258.1
(-2.3) 		1239.5
(-3) 		1393.9
(2.6) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 649
1 2 3 4 range
A 490.4
(2)
P 		412.8
(0.9)
P 		565.2
(3)
P 		125
(-3)
P 		440
B 578.5
(3)
P 		423.3
(0.6)
P 		480.1
(1.5)
P 		188.9
(-3)
P 		390
C 495.1
(0.3)
P 		475.7
(0.1)
P 		773.9
(3)
P 		151.7
(-3)
P 		622
avr 521.3
(1.9) 		437.3
(0.8) 		606.4
(3) 		155.2
(-3) 		451

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 247.5
(-3)
P 		476.4
(3)
P 		291.9
(-1.8)
P 		292.8
(-1.8)
P 		359.1
(-0.1)
P 		298.6
(-1.7)
P 		229
B 486.7
(1.4)
P 		416.5
(-0.7)
P 		360.4
(-2.4)
P 		340.7
(-3)
P 		537.8
(3)
P 		530.1
(2.8)
P 		197
C 545.1
(3)
P 		394.2
(-0.3)
P 		345
(-1.3)
P 		268.2
(-3)
P 		389.9
(-0.4)
P 		513.4
(2.3)
P 		277
avr 426.4
(2.1) 		429
(2.2) 		332.4
(-1.7) 		300.6
(-3) 		428.9
(2.2) 		447.4
(3) 		147
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173183_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26729
Sequence Type Exemplar sequence
Representative Public ID AV181386 NCBI
Target Description AV181386_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV181386 /DEF=Caenorhabditis elegans cDNA clone:yk619f6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059391(11), NM_182004(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059391 NCBI Caenorhabditis elegans phenylalanyl (F) tRNA Synthetase family member (frs-1) (frs-1) mRNA, complete cds. 11/11 A 
NM_182004 NCBI Caenorhabditis elegans phenylalanyl (F) tRNA Synthetase family member (frs-1) (frs-1) mRNA, complete cds. 11/11 A 
SNAP00000035003 ENSEMBL cdna:SNAP chromosome:CEL140:I:6219497:6222710:-1 10/11 None
GENEFINDER00000035017 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6220272:6222710:-1 11/11 None
T08B2.9a ENSEMBL cdna:known chromosome:CEL140:I:6220120:6222721:-1 gene:T08B2.9 11/11 None
T08B2.9b.2 ENSEMBL cdna:known chromosome:CEL140:I:6220272:6222353:-1 gene:T08B2.9 11/11 A 
T08B2.9b.3 ENSEMBL cdna:known chromosome:CEL140:I:6220272:6222712:-1 gene:T08B2.9 11/11 None
T08B2.9b.1 ENSEMBL cdna:known chromosome:CEL140:I:6220272:6222351:-1 gene:T08B2.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6220131-6220454(-) 92.0 92.0
Public Domain and Genome References
Ensembl T08B2.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491792 phenylalanyl (F) tRNA Synthetase family member (frs-1) [Caenorhabditis elegans] gb|AAG00015.1| Phenylalanyl (f) trna synthetase protein 1, isoform a [Caenorhabditis elegans] 0.0
blast CAE67141 Hypothetical protein CBG12564 [Caenorhabditis briggsae] 0.0
blast NP_871804 phenylalanyl (F) tRNA Synthetase family member (frs-1) [Caenorhabditis elegans] gb|AAO21394.1| Phenylalanyl (f) trna synthetase protein 1, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002319 EMBL-EBI Phenylalanyl-tRNA synthetase, class IIc 1.0E-126
Pfam IPR002319 EMBL-EBI Phenylalanyl-tRNA synthetase, class IIc 1.0E-126
Pfam IPR002319 EMBL-EBI Phenylalanyl-tRNA synthetase, class IIc 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173183_S_AT
tggaaactctggaatgttccgccctgaaatgcttctgccgatgggtttgccagctgatgt
gaatgtcgctggttatggtttgtccctggagcgcccgacaatgattaagtatggaattaa
caacattcgtgatcttttcggaagcaaaatcgatttgaatgtggtctacaataatccaat
ctgtagactcgacaagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAAACTCTGGAATGTTCCGCCCT 470 547 24 Antisense
TCTGGAATGTTCCGCCCTGAAATGC 633 607 31 Antisense
CCTGAAATGCTTCTGCCGATGGGTT 178 265 46 Antisense
CCGATGGGTTTGCCAGCTGATGTGA 77 263 61 Antisense
GCTGATGTGAATGTCGCTGGTTATG 171 297 76 Antisense
CTGGTTATGGTTTGTCCCTGGAGCG 439 239 92 Antisense
CCTGGAGCGCCCGACAATGATTAAG 74 267 108 Antisense
TAACAACATTCGTGATCTTTTCGGA 662 639 141 Antisense
GTGATCTTTTCGGAAGCAAAATCGA 580 483 152 Antisense
GAATGTGGTCTACAATAATCCAATC 264 333 180 Antisense
AATCCAATCTGTAGACTCGACAAGT 525 165 196 Antisense
Sequence Source Affymetrix Proprietary Database