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[173182_at]

Description AV181275_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV181275 /DEF=Caenorhabditis elegans cDNA clone:yk618b9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 50      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 27      6grp_total > 42      4grp_total > 60      6grp_cgc > 70      6grp_high1_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3203
1 2 3 4 range
A 8539.7
(2.4)
P 		7532.5
(0.5)
P 		8874.5
(3)
P 		5671.9
(-3)
P 		3203
B 7894.8
(2)
P 		7411.5
(0.4)
P 		8218.2
(3)
P 		6336.4
(-3)
P 		1882
C 7059.4
(0.4)
P 		7972.2
(3)
P 		7945.8
(2.9)
P 		5874.6
(-3)
P 		2098
avr 7831.3
(1.7) 		7638.7
(1.2) 		8346.2
(3) 		5961
(-3) 		2385

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4561
1 2 3 4 5 6 range
A 6247.5
(-1.3)
P 		6783.4
(-0.3)
P 		8503.8
(3)
P 		6644.9
(-0.5)
P 		7357.9
(0.8)
P 		5359.1
(-3)
P 		3145
B 7618
(0.9)
P 		6890.9
(-0)
P 		9190.5
(3)
P 		6838.4
(-0.1)
P 		6164.9
(-1)
P 		4629.8
(-3)
P 		4561
C 7166.4
(0.9)
P 		6681.8
(0.1)
P 		7595.6
(1.6)
P 		8454.8
(3)
P 		6486.9
(-0.2)
P 		4726.9
(-3)
P 		3728
avr 7010.6
(0.6) 		6785.4
(0.2) 		8430
(3) 		7312.7
(1.1) 		6669.9
(0) 		4905.3
(-3) 		3525
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2279
1 2 3 4 range
A 3109.3
(3)
P 		1635.1
(-0.9)
P 		2320
(0.9)
P 		830.2
(-3)
P 		2279
B 2517.6
(3)
P 		1830.5
(-0.5)
P 		1904.3
(-0.2)
P 		1350.3
(-3)
P 		1167
C 2336.9
(1.5)
P 		2292.1
(1.4)
P 		2769.3
(3)
P 		990.6
(-3)
P 		1779
avr 2654.6
(3) 		1919.2
(0.2) 		2331.2
(1.8) 		1057
(-3) 		1598

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1505
1 2 3 4 5 6 range
A 1151.7
(-3)
P 		2412.5
(3)
P 		1851.9
(0.3)
P 		1685.3
(-0.5)
P 		2220.3
(2.1)
P 		1304.8
(-2.3)
P 		1261
B 2650.7
(3)
P 		2293.7
(0.7)
P 		2231.7
(0.3)
P 		1811.2
(-2.4)
P 		2504.7
(2.1)
P 		1726.4
(-3)
P 		924
C 2656.3
(3)
P 		2038.8
(-0.5)
P 		1933.2
(-1)
P 		1700.3
(-2.3)
P 		2099.1
(-0.1)
P 		1582.4
(-3)
P 		1074
avr 2152.9
(2) 		2248.3
(2.8) 		2005.6
(0.8) 		1732.3
(-1.4) 		2274.7
(3) 		1537.9
(-3) 		737
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173182_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26720
Sequence Type Exemplar sequence
Representative Public ID AV181275 NCBI
Target Description AV181275_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV181275 /DEF=Caenorhabditis elegans cDNA clone:yk618b9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171128(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171128 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-3) (mlc-3) mRNA, complete cds. 11/11 None
F09F7.2a ENSEMBL cdna:known chromosome:CEL140:III:5564983:5567338:1 gene:F09F7.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175768 2 1 NM_171128 173182_at (11), 188483_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5566974-5567231(+) 85.67 85.67
Public Domain and Genome References
Ensembl F09F7.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741145 Myosin Light Chain family member (mlc-3) [Caenorhabditis elegans] gb|AAA50701.1| Myosin light chain protein 3, isoform a [Caenorhabditis elegans] sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin light chain alkali) gb|AAA28112.1| alkali myosin light chain 8.0E-83
blast CAE56371 Hypothetical protein CBG24046 [Caenorhabditis briggsae] 9.0E-79
blast NP_741146 Myosin Light Chain family member (mlc-3) [Caenorhabditis elegans] gb|AAM22061.1| Myosin light chain protein 3, isoform b [Caenorhabditis elegans] 7.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.025
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.13
Sequence
Target Sequence 
>C. ELEGANS:173182_AT
tttctccccgttccaaataacgatgttttacttttttgtctataaacccatcgtctagtc
gctcgttttcttctacgctatacacgtaagagattccactcgcgtgcgcgcattccgttt
tctgatccagaaaacgttttgcactttaaaaatcagcgcgcggttttctcttctttttct
ccaacacattcaagcctgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCTCCCCGTTCCAAATAACGATG 690 671 61 Antisense
TACTTTTTTGTCTATAAACCCATCG 562 643 89 Antisense
TCTATAAACCCATCGTCTAGTCGCT 300 605 99 Antisense
GCTCGTTTTCTTCTACGCTATACAC 608 295 121 Antisense
GCTATACACGTAAGAGATTCCACTC 190 303 137 Antisense
CACGTAAGAGATTCCACTCGCGTGC 453 199 143 Antisense
CGCATTCCGTTTTCTGATCCAGAAA 451 255 169 Antisense
GATCCAGAAAACGTTTTGCACTTTA 250 415 184 Antisense
GCACTTTAAAAATCAGCGCGCGGTT 6 315 201 Antisense
TAAAAATCAGCGCGCGGTTTTCTCT 27 641 207 Antisense
TTCTCCAACACATTCAAGCCTGTTT 635 693 237 Antisense
Sequence Source Affymetrix Proprietary Database