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[173172_s_at] cuticulin 1

Description AV183161_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV183161 /DEF=Caenorhabditis elegans cDNA clone:yk647d10 : 3prime end, single read.
Cluster 6grp_total > 24      4grp_total > 42      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 12.4
(-3)
A 		90.3
(3)
P 		62.8
(0.9)
P 		46.8
(-0.4)
A 		78
B 35.9
(-1.5)
A 		93.6
(3)
P 		69.2
(1.1)
P 		17
(-3)
A 		77
C 41.2
(-3)
A 		110.7
(3)
P 		90.2
(1.2)
P 		59.9
(-1.4)
A 		70
avr 29.8
(-3) 		98.2
(3) 		74.1
(0.9) 		41.2
(-2) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 54.8
(3)
P 		55
(3)
P 		52.9
(2.7)
P 		7.6
(-3)
A 		24.3
(-0.9)
A 		39.2
(1)
A 		47
B 95.3
(3)
P 		81.2
(1.5)
P 		83.5
(1.8)
P 		53.3
(-1.4)
P 		38.3
(-3)
P 		43.5
(-2.5)
A 		57
C 97.6
(3)
P 		53.8
(-0.4)
A 		35.5
(-1.9)
A 		65.2
(0.5)
A 		36.7
(-1.8)
A 		21.2
(-3)
A 		76
avr 82.6
(3) 		63.3
(0.7) 		57.3
(-0.1) 		42
(-1.9) 		33.1
(-3) 		34.6
(-2.8) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 4.5
(-3)
A 		19.6
(3)
P 		16.4
(1.7)
P 		6.8
(-2.1)
A 		15
B 11.4
(-0.6)
A 		23.1
(3)
P 		16
(0.8)
P 		3.6
(-3)
A 		20
C 13.6
(-2)
A 		31.8
(3)
P 		31.5
(2.9)
P 		10.1
(-3)
A 		22
avr 9.8
(-2) 		24.8
(3) 		21.3
(1.8) 		6.8
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 10.1
(-0.2)
P 		19.6
(3)
P 		11.5
(0.3)
P 		1.9
(-3)
A 		7.3
(-1.2)
A 		9.5
(-0.4)
A 		18
B 33.1
(3)
P 		27
(1.1)
P 		20.3
(-1)
P 		14.1
(-3)
P 		15.6
(-2.5)
P 		16.2
(-2.3)
A 		19
C 36.2
(3)
P 		16.4
(-1.1)
A 		9
(-2.6)
A 		13.1
(-1.8)
A 		11.9
(-2)
A 		7.1
(-3)
A 		29
avr 26.5
(3) 		21
(1) 		13.6
(-1.6) 		9.7
(-3) 		11.6
(-2.3) 		10.9
(-2.6) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173172_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26845
Sequence Type Exemplar sequence
Representative Public ID AV183161 NCBI
Target Description AV183161_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV183161 /DEF=Caenorhabditis elegans cDNA clone:yk647d10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063892(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063892 NCBI Caenorhabditis elegans M28.1 (M28.1) mRNA, complete cds. 11/11 A 
SNAP00000007399 ENSEMBL cdna:SNAP chromosome:CEL140:II:10622943:10625770:1 11/11 None
GENEFINDER00000007414 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10622216:10625770:1 11/11 None
M28.1 ENSEMBL cdna:known chromosome:CEL140:II:10622192:10626345:1 gene:M28.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10625498-10625732(+) 75.33 75.33
Public Domain and Genome References
Gene Title cuticulin 1
Ensembl M28.1
Entrez Gene 174640 Entrez gene
SwissProt Q21573 EMBL-EBI
WormBase CE23881 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90131 Hypothetical protein M28.1 [Caenorhabditis elegans] ref|NP_496293.1| M28.1 [Caenorhabditis elegans] 0.0
blast CAE59642 Hypothetical protein CBG03056 [Caenorhabditis briggsae] 0.0
blast CAA91480 Hypothetical protein T04F8.4 [Caenorhabditis elegans] ref|NP_509946.1| T04F8.4 [Caenorhabditis elegans] 2.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.6E-31
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.6E-31
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.6E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496293.1 1 524-546
M28.1 1 524-546
SNAP00000007399 1 518-540
GENEFINDER00000007414 1 549-571
Sequence
Target Sequence 
>C. ELEGANS:173172_S_AT
ctctacatgcaaatagtcttactgtatttgacattgattcatcaaaaactgatacaaatc
aactactaccaacacctgcattattacggaaaacatttgaagaatacgagagaactttct
gtgtttctgttgcaagttttggaatacttatatcngccttcaacgttctttgcaactatt
tcaatt

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTACATGCAAATAGTCTTACTGT 472 229 25 Antisense
GTCTTACTGTATTTGACATTGATTC 408 469 40 Antisense
GATACAAATCAACTACTACCAACAC 468 423 75 Antisense
AACTACTACCAACACCTGCATTATT 305 139 85 Antisense
CTACCAACACCTGCATTATTACGGA 275 225 90 Antisense
CACCTGCATTATTACGGAAAACATT 656 197 97 Antisense
ACGAGAGAACTTTCTGTGTTTCTGT 466 93 130 Antisense
GAACTTTCTGTGTTTCTGTTGCAAG 695 349 136 Antisense
CTGTGTTTCTGTTGCAAGTTTTGGA 453 239 143 Antisense
CTTCAACGTTCTTTGCAACTATTTC 376 221 182 Antisense
AACGTTCTTTGCAACTATTTCAATT 190 145 186 Antisense
Sequence Source Affymetrix Proprietary Database