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[173163_s_at]

Description AV184275_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV184275 /DEF=Caenorhabditis elegans cDNA clone:yk662c7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 92      6grp_total > 86      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1690
1 2 3 4 range
A 318.1
(-3)
P 		330.1
(-3)
P 		371.5
(-2.8)
P 		1934
(3)
P 		1616
B 271.7
(-2.9)
P 		244.3
(-3)
P 		358
(-2.5)
P 		1513.6
(3)
P 		1269
C 328.6
(-2.7)
P 		267.3
(-3)
P 		337
(-2.7)
P 		1714.7
(3)
P 		1447
avr 306.1
(-2.9) 		280.6
(-3) 		355.5
(-2.7) 		1720.8
(3) 		1440

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 247.2
(-0.9)
P 		172.7
(-3)
P 		213.3
(-1.9)
P 		237.8
(-1.2)
P 		309.2
(0.8)
P 		387.2
(3)
P 		215
B 180.7
(-3)
P 		203.9
(-2.4)
P 		326.3
(0.5)
P 		299.8
(-0.1)
P 		306.4
(0)
P 		428.8
(3)
P 		248
C 205.2
(-2.3)
P 		189.2
(-3)
P 		235
(-1.1)
P 		325.2
(2.6)
P 		294.3
(1.3)
P 		334.4
(3)
P 		145
avr 211
(-2.3) 		188.6
(-3) 		258.2
(-0.9) 		287.6
(0) 		303.3
(0.5) 		383.5
(3) 		195
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 262
1 2 3 4 range
A 115.8
(-1.7)
P 		71.6
(-3)
P 		97.1
(-2.3)
P 		283.1
(3)
P 		212
B 86.7
(-2.4)
P 		60.3
(-3)
P 		83
(-2.5)
P 		322.5
(3)
P 		262
C 108.8
(-2.1)
P 		76.8
(-3)
P 		117.5
(-1.8)
P 		289.1
(3)
P 		212
avr 103.8
(-2.1) 		69.6
(-3) 		99.2
(-2.2) 		298.2
(3) 		229

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 45.6
(-3)
P 		61.4
(-1.1)
P 		46.4
(-2.9)
P 		60.3
(-1.2)
P 		93.3
(2.9)
P 		94.3
(3)
P 		49
B 62.9
(-3)
P 		67.9
(-2.7)
P 		79.2
(-2)
P 		79.4
(-2)
P 		124.5
(0.8)
P 		159.9
(3)
P 		97
C 76.1
(-1)
P 		57.7
(-3)
P 		59.8
(-2.8)
P 		65.4
(-2.1)
P 		95.2
(1.2)
P 		111.9
(3)
P 		54
avr 61.5
(-3) 		62.3
(-2.9) 		61.8
(-3) 		68.4
(-2.3) 		104.3
(1.2) 		122
(3) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173163_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26870
Sequence Type Exemplar sequence
Representative Public ID AV184275 NCBI
Target Description AV184275_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV184275 /DEF=Caenorhabditis elegans cDNA clone:yk662c7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063871(11), NM_182124(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063871 NCBI Caenorhabditis elegans R166.5a (R166.5) mRNA, complete cds. 11/11 A 
NM_182124 NCBI Caenorhabditis elegans R166.5b (R166.5) mRNA, complete cds. 11/11 A 
SNAP00000017515 ENSEMBL cdna:SNAP chromosome:CEL140:II:10544267:10547627:-1 11/11 None
GENEFINDER00000017521 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10544267:10547627:-1 11/11 None
R166.5a ENSEMBL cdna:known chromosome:CEL140:II:10542337:10548820:-1 gene:R166.5 11/11 A 
R166.5b ENSEMBL cdna:known chromosome:CEL140:II:10544267:10554954:-1 gene:R166.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10544198-10544508(-) 85.67 85.67
Public Domain and Genome References
Ensembl R166.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90665 Hypothetical protein R166.5a [Caenorhabditis elegans] ref|NP_496272.1| R166.5a [Caenorhabditis elegans] 0.0
blast CAD59152 Hypothetical protein R166.5b [Caenorhabditis elegans] ref|NP_871924.1| R166.5b [Caenorhabditis elegans] 0.0
blast CAE59656 Hypothetical protein CBG03074 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-64
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 5.0E-70
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 3.0E-70
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 2.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Pfam IPR000719 EMBL-EBI Protein kinase 5.3E-84
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Sequence
Target Sequence 
>C. ELEGANS:173163_S_AT
gatctgttctccaccaatggtgtttgtcgatatgccatctattcaactgttcggtactgg
agcattgcttacttcagttcaaatgacgcctcgtcacaccaccgaagacgacgcatcatt
gaagtcgtttgaggacgagcaggagaatgcgaatcccatccatcgcatcgagactcaggt
caacgtcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTGTTCTCCACCAATGGTGTTT 78 419 15 Antisense
GGTGTTTGTCGATATGCCATCTATT 412 499 33 Antisense
TATGCCATCTATTCAACTGTTCGGT 689 657 45 Antisense
AACTGTTCGGTACTGGAGCATTGCT 229 143 59 Antisense
GGAGCATTGCTTACTTCAGTTCAAA 268 521 73 Antisense
TTCAAATGACGCCTCGTCACACCAC 127 687 92 Antisense
ACACCACCGAAGACGACGCATCATT 568 109 110 Antisense
GACGCATCATTGAAGTCGTTTGAGG 671 379 124 Antisense
AGGAGAATGCGAATCCCATCCATCG 701 55 155 Antisense
CCATCCATCGCATCGAGACTCAGGT 457 271 170 Antisense
GCATCGAGACTCAGGTCAACGTCTA 35 311 179 Antisense
Sequence Source Affymetrix Proprietary Database