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[173143_s_at] cAMP-dependant protein kinase

Description AV184878_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV184878 /DEF=Caenorhabditis elegans cDNA clone:yk670f5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 70      6grp_total > 05      4grp_total > 21      6grp_highest_sub > De      6grp_high1_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 608
1 2 3 4 range
A 451.2
(2.1)
P 		311.8
(-0.4)
P 		168.3
(-3)
P 		503.6
(3)
P 		335
B 523.4
(0.7)
P 		262.5
(-1.8)
P 		145.2
(-3)
P 		752.9
(3)
P 		608
C 519.1
(1.5)
P 		250.1
(-2.5)
P 		213.1
(-3)
P 		617
(3)
P 		404
avr 497.9
(1.3) 		274.8
(-1.7) 		175.5
(-3) 		624.5
(3) 		449

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 443
1 2 3 4 5 6 range
A 422.9
(3)
P 		377.7
(2.1)
P 		232.8
(-0.9)
P 		215.5
(-1.2)
P 		287.8
(0.2)
P 		128.5
(-3)
P 		294
B 571.5
(3)
P 		313.3
(-0.6)
P 		315.5
(-0.6)
P 		269.6
(-1.2)
P 		208.7
(-2.1)
P 		141.6
(-3)
P 		430
C 510.4
(3)
P 		363.9
(0.6)
P 		212.6
(-1.9)
P 		261.4
(-1.1)
P 		143.9
(-3)
P 		248.3
(-1.3)
P 		367
avr 501.6
(3) 		351.6
(0.3) 		253.6
(-1.5) 		248.8
(-1.6) 		213.5
(-2.3) 		172.8
(-3) 		329
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 164.3
(3)
P 		67.7
(-1.8)
P 		44
(-3)
P 		73.7
(-1.5)
P 		120
B 166.9
(3)
P 		64.8
(-1.6)
P 		33.6
(-3)
P 		160.4
(2.7)
P 		133
C 171.8
(3)
P 		71.9
(-3)
P 		74.3
(-2.9)
P 		104
(-1.1)
P 		100
avr 167.7
(3) 		68.1
(-2.1) 		50.6
(-3) 		112.7
(0.2) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 78
(-0.3)
P 		134.3
(3)
P 		50.7
(-1.9)
P 		54.7
(-1.6)
P 		86.8
(0.2)
P 		31.3
(-3)
P 		103
B 198.9
(3)
P 		104.3
(-0.9)
P 		76.6
(-2)
P 		71.4
(-2.2)
P 		84.8
(-1.7)
P 		52.8
(-3)
P 		146
C 189.2
(3)
P 		111
(-0.3)
P 		54.1
(-2.7)
P 		52.6
(-2.7)
P 		46.6
(-3)
P 		83.1
(-1.5)
P 		143
avr 155.4
(3) 		116.5
(0.7) 		60.5
(-2.7) 		59.6
(-2.8) 		72.7
(-2) 		55.7
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173143_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26896
Sequence Type Exemplar sequence
Representative Public ID AV184878 NCBI
Target Description AV184878_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV184878 /DEF=Caenorhabditis elegans cDNA clone:yk670f5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066893(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066893 NCBI Caenorhabditis elegans M03C11.1 (M03C11.1) mRNA, complete cds. 11/11 A 
GENEFINDER00000000557 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10392753:10395955:-1 9/11 None
SNAP00000000514 ENSEMBL cdna:SNAP chromosome:CEL140:III:10393347:10395438:-1 11/11 None
M03C11.1 ENSEMBL cdna:known chromosome:CEL140:III:10393275:10395959:-1 gene:M03C11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10393355-10393627(-) 76.0 76.0
Public Domain and Genome References
Gene Title cAMP-dependant protein kinase
Ensembl M03C11.1
Entrez Gene 176456 Entrez gene
SwissProt Q21483 EMBL-EBI
WormBase CE03492 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597354_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFA.8447.1.A1_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFAAFFX.24090.1.S1_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFAAFFX.24090.1.S1_S_AT similar to serine/threonine kinase 32B cfa
CHICKEN:GGA.11676.1.S1_S_AT serine/threonine kinase 32B gga
CHICKEN:GGAAFFX.24572.1.S1_AT serine/threonine kinase 32B gga
CHICKEN:GGAAFFX.9537.1.S1_S_AT serine/threonine kinase 32B gga
HG-U133_PLUS_2:219686_AT serine/threonine kinase 32B hs
HG-U133A:219686_AT serine/threonine kinase 32B hs
HG-FOCUS:219686_AT serine/threonine kinase 32B hs
HG-U133A_2:219686_AT serine/threonine kinase 32B hs
HU35KSUBC:RC_W72870_AT serine/threonine kinase 32B hs
U133_X3P:G8923753_3P_AT serine/threonine kinase 32B hs
HG-U95B:51856_AT serine/threonine kinase 32B hs
HG-U133_PLUS_2:240679_AT Serine/threonine kinase 32B hs
HG-U133B:240679_AT Serine/threonine kinase 32B hs
HG-U95C:49543_AT Serine/threonine kinase 32B hs
HG-U95D:74216_AT Serine/threonine kinase 32B hs
U133_X3P:HS.232204.0.A1_3P_AT Serine/threonine kinase 32B hs
MOE430B:1431236_AT serine/threonine kinase 32B mm
MOUSE430_2:1431236_AT serine/threonine kinase 32B mm
MOE430A:1452420_AT serine/threonine kinase 32B mm
MOUSE430_2:1452420_AT serine/threonine kinase 32B mm
MOUSE430A_2:1452420_AT serine/threonine kinase 32B mm
MU11KSUBB:MSA.15267.0_S_AT serine/threonine kinase 32B mm
MG-U74CV2:131549_AT serine/threonine kinase 32B mm
MG-U74BV2:109434_AT Serine/threonine kinase 32B, mRNA (cDNA clone MGC:67312 IMAGE:5715729) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88953 Hypothetical protein M03C11.1 [Caenorhabditis elegans] ref|NP_499294.1| M03C11.1 [Caenorhabditis elegans] 0.0
blast CAE71477 Hypothetical protein CBG18395 [Caenorhabditis briggsae] 0.0
blast CAA88953 Hypothetical protein M03C11.1 [Caenorhabditis elegans] ref|NP_499294.1| M03C11.1 [Caenorhabditis elegans] 1.0E-175
blast CAE71477 Hypothetical protein CBG18395 [Caenorhabditis briggsae] 1.0E-167
blast AAH58412 Serine/threonine kinase 32B [Mus musculus] gb|AAH56396.1| Serine/threonine kinase 32B [Mus musculus] gb|AAH52404.1| Serine/threonine kinase 32B [Mus musculus] ref|NP_071861.1| serine/threonine kinase 32B [Mus musculus] 2.0E-93
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-63
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 4.0E-58
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 2.0E-74
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-79
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-61
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-79
Sequence
Target Sequence 
>C. ELEGANS:173143_S_AT
tcgccagtgttcattccttgtaaagagggtctcaactgtgatccgatgtatgagttggaa
gaacgtattcttgtctcaacaccaattcatcggcggagaaccaatcataataatagctca
gggagaagctcgagtgagccgcaaaatgccgcgttggtcgaggtttcaaaggcgttcatt
gatttttccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGCCAGTGTTCATTCCTTGTAAAG 149 599 65 Antisense
CATTCCTTGTAAAGAGGGTCTCAAC 540 215 76 Antisense
GGGTCTCAACTGTGATCCGATGTAT 361 497 91 Antisense
AGAACGTATTCTTGTCTCAACACCA 530 73 124 Antisense
GTCTCAACACCAATTCATCGGCGGA 647 469 137 Antisense
AATTCATCGGCGGAGAACCAATCAT 230 177 148 Antisense
GAACCAATCATAATAATAGCTCAGG 290 345 162 Antisense
GGGAGAAGCTCGAGTGAGCCGCAAA 479 495 185 Antisense
AGTGAGCCGCAAAATGCCGCGTTGG 584 57 197 Antisense
GCCGCGTTGGTCGAGGTTTCAAAGG 259 283 212 Antisense
TCAAAGGCGTTCATTGATTTTTCCC 569 627 230 Antisense
Sequence Source Affymetrix Proprietary Database