[Home] | [Total List]

[173115_s_at] glutathione synthase

Description AV182552_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV182552 /DEF=Caenorhabditis elegans cDNA clone:yk639e6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 32      6grp_total > 31      4grp_total > 57      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 1100.7
(-3)
P 		1559.1
(3)
P 		1424.5
(1.2)
P 		1191.2
(-1.8)
P 		458
B 1078.4
(-2)
P 		1524.5
(3)
P 		1520.1
(3)
P 		988.6
(-3)
P 		536
C 1104
(-2.1)
P 		1339.2
(1.8)
P 		1414.7
(3)
P 		1052.3
(-3)
P 		362
avr 1094.4
(-2.7) 		1474.3
(3) 		1453.1
(2.7) 		1077.4
(-3) 		397

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1050
1 2 3 4 5 6 range
A 1157.2
(-0.3)
P 		1189.7
(-0.1)
P 		1740.1
(3)
P 		1230.8
(0.1)
P 		965
(-1.4)
P 		690.1
(-3)
P 		1050
B 1357.5
(1.9)
P 		1376.9
(2.2)
P 		1445.8
(3)
P 		1372.2
(2.1)
P 		1135.9
(-0.8)
P 		958.4
(-3)
P 		487
C 1125.9
(0.1)
P 		1345
(3)
P 		1068.2
(-0.7)
P 		1202.2
(1.1)
P 		898.6
(-3)
P 		963
(-2.1)
P 		446
avr 1213.5
(0.8) 		1303.9
(1.7) 		1418
(3) 		1268.4
(1.4) 		999.8
(-1.6) 		870.5
(-3) 		548
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 400.8
(3)
P 		338.4
(1.3)
P 		372.4
(2.2)
P 		174.4
(-3)
P 		226
B 343.9
(1.8)
P 		376.5
(3)
P 		352.3
(2.1)
P 		210.7
(-3)
P 		166
C 365.4
(0.6)
P 		385
(0.9)
P 		493.1
(3)
P 		177.4
(-3)
P 		316
avr 370
(2) 		366.6
(1.9) 		405.9
(3) 		187.5
(-3) 		218

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 304
1 2 3 4 5 6 range
A 213.3
(-1.9)
P 		423.1
(3)
P 		379
(2)
P 		312.2
(0.4)
P 		291.2
(-0.1)
P 		168
(-3)
P 		255
B 472.4
(3)
P 		458.3
(2.3)
P 		351.1
(-3)
P 		363.5
(-2.4)
P 		461.5
(2.5)
P 		357.4
(-2.7)
P 		121
C 417.3
(3)
P 		410.4
(2.8)
P 		271.9
(-2)
P 		241.8
(-3)
P 		290.8
(-1.3)
P 		322.4
(-0.2)
P 		176
avr 367.7
(0.4) 		430.6
(3) 		334
(-0.9) 		305.8
(-2.1) 		347.8
(-0.4) 		282.6
(-3) 		148
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173115_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26808
Sequence Type Exemplar sequence
Representative Public ID AV182552 NCBI
Target Description AV182552_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV182552 /DEF=Caenorhabditis elegans cDNA clone:yk639e6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063610(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063610 NCBI Caenorhabditis elegans M176.2 (M176.2) mRNA, complete cds. 11/11 A 
SNAP00000025000 ENSEMBL cdna:SNAP chromosome:CEL140:II:9414623:9416342:-1 11/11 None
GENEFINDER00000025009 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9414623:9416342:-1 11/11 None
M176.2.1 ENSEMBL cdna:known chromosome:CEL140:II:9414497:9416343:-1 gene:M176.2 11/11 None
M176.2.2 ENSEMBL cdna:known chromosome:CEL140:II:9414534:9416346:-1 gene:M176.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9414567-9414866(-) 83.0 83.0
Public Domain and Genome References
Gene Title glutathione synthase
Ensembl M176.2
Entrez Gene 174493 Entrez gene
SwissProt Q21549 EMBL-EBI
WormBase CE18087 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15211_S_AT glutathione synthetase (GSH2) at
ATH1-121501:246785_AT glutathione synthetase (GSH2) at
CANINE:1601155_AT glutathione synthetase cfa
CANINE:1589481_X_AT glutathione synthetase cfa
CANINE_2:CFA.3089.1.A1_AT glutathione synthetase cfa
CANINE_2:CFAAFFX.12357.1.S1_AT glutathione synthetase cfa
CANINE_2:CFAAFFX.12357.1.S1_S_AT glutathione synthetase cfa
DROSOPHILA_2:1633036_S_AT dm
DROSGENOME1:145269_AT dm
DROSOPHILA_2:1633036_S_AT Glutathione Synthetase dm
DROSGENOME1:145269_AT Glutathione Synthetase dm
DROSOPHILA_2:1627321_X_AT Glutathione Synthetase dm
CHICKEN:GGA.5371.1.S1_AT similar to Glutathione synthetase (Glutathione synthase) (GSH synthetase) (GSH-S) gga
CHICKEN:GGAAFFX.25794.2.S1_S_AT similar to Glutathione synthetase (Glutathione synthase) (GSH synthetase) (GSH-S) gga
HG-U133_PLUS_2:201415_AT glutathione synthetase hs
HG-U133A:201415_AT glutathione synthetase hs
HG-U133A_2:201415_AT glutathione synthetase hs
HG-FOCUS:201415_AT glutathione synthetase hs
HG-U133_PLUS_2:211630_S_AT glutathione synthetase hs
HG-U133A:211630_S_AT glutathione synthetase hs
HG-U133A_2:211630_S_AT glutathione synthetase hs
HUGENEFL:U34683_AT glutathione synthetase hs
U133_X3P:G886283_3P_S_AT glutathione synthetase hs
U133_X3P:G4504168_3P_AT glutathione synthetase hs
HG-U95AV2:38386_R_AT glutathione synthetase hs
HG-U95D:74726_AT glutathione synthetase hs
HG-U95AV2:41631_F_AT Glutathione synthetase hs
MU11KSUBA:U35456_S_AT glutathione synthetase mm
MG-U74AV2:101425_AT glutathione synthetase mm
MG-U74AV2:162246_R_AT glutathione synthetase mm
MOE430A:1448273_AT glutathione synthetase mm
MOUSE430A_2:1448273_AT glutathione synthetase mm
MOUSE430_2:1448273_AT glutathione synthetase mm
MOE430B:1441931_X_AT glutathione synthetase mm
MOUSE430_2:1441931_X_AT glutathione synthetase mm
MU19KSUBA:TC19090_S_AT Glutathione synthetase, mRNA (cDNA clone MGC:6012 IMAGE:3593913) mm
MU19KSUBA:TC20234_AT Glutathione synthetase, mRNA (cDNA clone MGC:6012 IMAGE:3593913) mm
RT-U34:L38615_AT glutathione synthetase rn
RG-U34A:L38615_AT glutathione synthetase rn
RT-U34:L38615_G_AT glutathione synthetase rn
RG-U34A:L38615_G_AT glutathione synthetase rn
RG-U34C:RC_AI179190_S_AT glutathione synthetase rn
RAE230A:1370365_AT glutathione synthetase rn
RAT230_2:1370365_AT glutathione synthetase rn
YEAST_2:1778316_AT Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6750 glutathione biosynthesis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4363 glutathione synthase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16740 transferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01655 Hypothetical protein M176.2 [Caenorhabditis elegans] ref|NP_496011.1| M176.2 [Caenorhabditis elegans] 0.0
blast CAE67815 Hypothetical protein CBG13395 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004887 EMBL-EBI Eukaryotic glutathione synthase 3.5E-70
Pfam IPR005615 EMBL-EBI Eukaryotic glutathione synthase, ATP binding domain 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173115_S_AT
tcttgcaaagaagccgatcacatttgctaaggctgttagtgaacttggagtgtatggtta
tgcatttggaaggaaggatgcacctgagcttaagactgctgggcatttgctccgaacgaa
accggaatccacagctatgggtggagtagccgccggacatgctgttgtcgacaccccatt
cctctacgaatttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTGCAAAGAAGCCGATCACATTT 54 615 14 Antisense
CCGATCACATTTGCTAAGGCTGTTA 643 263 27 Antisense
AAGGCTGTTAGTGAACTTGGAGTGT 453 159 42 Antisense
TGGAGTGTATGGTTATGCATTTGGA 70 551 59 Antisense
GGAAGGAAGGATGCACCTGAGCTTA 489 531 81 Antisense
GCACCTGAGCTTAAGACTGCTGGGC 568 313 93 Antisense
TTAAGACTGCTGGGCATTTGCTCCG 317 683 103 Antisense
GGCATTTGCTCCGAACGAAACCGGA 214 511 115 Antisense
GAAACCGGAATCCACAGCTATGGGT 129 359 131 Antisense
CAGCTATGGGTGGAGTAGCCGCCGG 34 193 145 Antisense
CACCCCATTCCTCTACGAATTTATT 57 201 185 Antisense
Sequence Source Affymetrix Proprietary Database