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[173084_at] translation initiation factor

Description CEK109CYR_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=D72778 /DEF=C.elegans cDNA clone yk109c11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 37      6grp_total > 04      4grp_total > 57      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3370
1 2 3 4 range
A 7082.9
(0.2)
P 		8001.3
(2.1)
P 		8421.9
(3)
P 		5590.1
(-3)
P 		2832
B 5052.4
(-3)
P 		6154.6
(2)
P 		6377.7
(3)
P 		5398.5
(-1.4)
P 		1325
C 6036.6
(-2.7)
P 		7453.2
(2.7)
P 		7531.8
(3)
P 		5948
(-3)
P 		1584
avr 6057.3
(-1.6) 		7203
(2.2) 		7443.8
(3) 		5645.5
(-3) 		1798

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2830
1 2 3 4 5 6 range
A 6618.8
(3)
P 		4794.9
(-0.9)
P 		5470.8
(0.6)
P 		5408.6
(0.4)
P 		4766.8
(-0.9)
P 		3788.8
(-3)
P 		2830
B 6440.9
(3)
P 		5061.1
(-0.5)
P 		4050.1
(-3)
P 		5136.6
(-0.3)
P 		5322.9
(0.2)
P 		4755.3
(-1.2)
P 		2391
C 4832.1
(-1.2)
P 		6593.9
(3)
P 		4722
(-1.5)
P 		4979.9
(-0.9)
P 		4080.9
(-3)
P 		4587.5
(-1.8)
P 		2513
avr 5963.9
(3) 		5483.3
(1.2) 		4747.6
(-1.6) 		5175
(0) 		4723.5
(-1.7) 		4377.2
(-3) 		1587
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1807
1 2 3 4 range
A 2578.9
(3)
P 		1736.9
(0.1)
P 		2201.6
(1.7)
P 		818.3
(-3)
P 		1761
B 1611.2
(3)
P 		1520
(1.8)
P 		1477.9
(1.3)
P 		1150.4
(-3)
P 		461
C 1998.3
(0.7)
P 		2142.8
(1.2)
P 		2625.1
(3)
P 		1002.9
(-3)
P 		1622
avr 2062.8
(2.8) 		1799.9
(1.4) 		2101.5
(3) 		990.5
(-3) 		1111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1319
1 2 3 4 5 6 range
A 1220.1
(-0.7)
P 		1705.3
(3)
P 		1191.4
(-0.9)
P 		1371.7
(0.4)
P 		1438.4
(1)
P 		922.5
(-3)
P 		783
B 2241.1
(3)
P 		1684.6
(0.3)
P 		983.5
(-3)
P 		1360.5
(-1.2)
P 		2162.6
(2.6)
P 		1773.2
(0.8)
P 		1258
C 1791.1
(1.7)
P 		2012
(3)
P 		1201.8
(-1.8)
P 		1001.5
(-3)
P 		1320.5
(-1.1)
P 		1535.7
(0.2)
P 		1011
avr 1750.8
(2.6) 		1800.6
(3) 		1125.6
(-3) 		1244.6
(-1.9) 		1640.5
(1.6) 		1410.5
(-0.5) 		675
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173084_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27461
Sequence Type Exemplar sequence
Representative Public ID CEK109CYR NCBI
Target Description CEK109CYR_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=D72778 /DEF=C.elegans cDNA clone yk109c11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068249(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068249 NCBI Caenorhabditis elegans H06H21.3 (H06H21.3) mRNA, complete cds. 10/11 None
H06H21.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:4810922:4811948:1 gene:H06H21.3 10/11 None
H06H21.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:4810924:4811959:1 gene:H06H21.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4811701-4811965(+) 88.0 88.0
Public Domain and Genome References
Gene Title translation initiation factor
Ensembl H06H21.3
Entrez Gene 177254 Entrez gene
SwissProt Q9TXU7 EMBL-EBI
WormBase CE17962 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15296_AT eukaryotic translation initiation factor 1A, putative / eIF-1A, putative / eIF-4C, putative at
ATH1-121501:263810_AT eukaryotic translation initiation factor 1A, putative / eIF-1A, putative / eIF-4C, putative at
CANINE_2:CFA.18497.1.S1_AT similar to eukaryotic translation initiation factor 1A, Y-linked cfa
CANINE_2:CFA.18497.1.S1_S_AT similar to eukaryotic translation initiation factor 1A, Y-linked cfa
CANINE_2:CFAAFFX.20307.1.S1_AT similar to eukaryotic translation initiation factor 1A, Y-linked cfa
DROSOPHILA_2:1640435_AT Eukaryotic initiation factor 1A dm
DROSGENOME1:150126_AT Eukaryotic initiation factor 1A dm
CHICKEN:GGA.15791.1.S1_S_AT eukaryotic translation initiation factor 1A, X-linked gga
CHICKEN:GGA.15791.2.S1_S_AT eukaryotic translation initiation factor 1A, X-linked gga
CHICKEN:GGA.15791.2.S1_X_AT eukaryotic translation initiation factor 1A, X-linked gga
CHICKEN:GGA.15791.3.S1_AT eukaryotic translation initiation factor 1A, X-linked gga
CHICKEN:GGA.15791.2.S1_AT Similar to Eukaryotic translation initiation factor 1A, Y-chromosomal (eIF-1A Y isoform) (eIF-4C) gga
HG-U133_PLUS_2:201017_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A:201017_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A_2:201017_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133_PLUS_2:201019_S_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A_2:201019_S_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A:201019_S_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133_PLUS_2:201018_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A:201018_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A_2:201018_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-FOCUS:201018_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U95AV2:663_AT eukaryotic translation initiation factor 1A, X-linked hs
HC-G110:663_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133_PLUS_2:201016_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A:201016_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U133A_2:201016_AT eukaryotic translation initiation factor 1A, X-linked hs
HU35KSUBA:AA203513_AT eukaryotic translation initiation factor 1A, X-linked hs
HU35KSUBA:RC_AA421250_AT eukaryotic translation initiation factor 1A, X-linked hs
HU35KSUBB:RC_N71704_AT eukaryotic translation initiation factor 1A, X-linked hs
HU35KSUBC:RC_AA281733_AT eukaryotic translation initiation factor 1A, X-linked hs
HUGENEFL:L18960_AT eukaryotic translation initiation factor 1A, X-linked hs
U133_X3P:G4503498_3P_A_AT eukaryotic translation initiation factor 1A, X-linked hs
U133_X3P:HS.4310.0.S4_3P_AT eukaryotic translation initiation factor 1A, X-linked hs
U133_X3P:HS.4310.0.S3_3P_AT eukaryotic translation initiation factor 1A, X-linked hs
U133_X3P:HS.4310.0.S2_3P_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U95AV2:34278_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U95AV2:34813_AT eukaryotic translation initiation factor 1A, X-linked hs
HG-U95B:49153_AT eukaryotic translation initiation factor 1A, X-linked hs
MG-U74AV2:160111_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74BV2:163794_R_AT eukaryotic translation initiation factor 1A, Y-linked mm
MU11KSUBB:MSA.6343.0_S_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74AV2:93358_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74BV2:109662_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74BV2:165199_F_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74BV2:164324_F_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74CV2:168528_F_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOE430A:1419736_A_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOUSE430A_2:1419736_A_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOUSE430_2:1419736_A_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOE430A:1449576_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOUSE430A_2:1449576_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOUSE430_2:1449576_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOE430B:1437071_AT eukaryotic translation initiation factor 1A, Y-linked mm
MOUSE430_2:1437071_AT eukaryotic translation initiation factor 1A, Y-linked mm
MG-U74BV2:112968_AT Eukaryotic translation initiation factor 1A, Y-linked, mRNA (cDNA clone MGC:29011 IMAGE:3156134) mm
MU19KSUBA:TC25748_AT Eukaryotic translation initiation factor 1A, Y-linked, mRNA (cDNA clone MGC:29011 IMAGE:3156134) mm
MU19KSUBC:TC36702_AT Eukaryotic translation initiation factor 1A, Y-linked, mRNA (cDNA clone MGC:29011 IMAGE:3156134) mm
MU19KSUBC:TC36702_G_AT Eukaryotic translation initiation factor 1A, Y-linked, mRNA (cDNA clone MGC:29011 IMAGE:3156134) mm
MU19KSUBC:TC39579_AT Eukaryotic translation initiation factor 1A, Y-linked, mRNA (cDNA clone MGC:29011 IMAGE:3156134) mm
RG-U34B:RC_AI009376_AT similar to eukaryotic translation initiation factor 1A, Y-linked rn
RAE230A:1372331_AT similar to eukaryotic translation initiation factor 1A, Y-linked rn
RAT230_2:1372331_AT similar to eukaryotic translation initiation factor 1A, Y-linked rn
YEAST_2:1780076_AT Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29845 Hypothetical protein H06H21.3 [Caenorhabditis elegans] ref|NP_500650.1| H06H21.3 [Caenorhabditis elegans] 4.0E-71
blast CAE66391 Hypothetical protein CBG11654 [Caenorhabditis briggsae] 3.0E-68
blast CAE58282 Hypothetical protein CBG01389 [Caenorhabditis briggsae] 2.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR001253 EMBL-EBI Eukaryotic initiation factor 1A (eIF-1A) 2.2E-59
Sequence
Target Sequence 
>C. ELEGANS:173084_AT
aaaaatccattgtttctcttctcgatccntttcagacattatcttcccatcttctctgtc
tcccgaaattccgttacttgaatgctacaaagtgaaataattcgattgtggtgtccgatg
atcaacacaacctcaaggtttcggtccatcggacaccacttgatgaaacaactcatttat
tgcaaattatcccgtattccacta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATCCATTGTTTCTCTTCTCGA 697 129 79 Antisense
AAATTCCGTTACTTGAATGCTACAA 630 115 144 Antisense
GTGAAATAATTCGATTGTGGTGTCC 445 479 170 Antisense
TCGATTGTGGTGTCCGATGATCAAC 70 605 180 Antisense
GTCCGATGATCAACACAACCTCAAG 137 477 191 Antisense
ACAACCTCAAGGTTTCGGTCCATCG 86 113 205 Antisense
TCAAGGTTTCGGTCCATCGGACACC 205 631 211 Antisense
GGTCCATCGGACACCACTTGATGAA 596 501 221 Antisense
GGACACCACTTGATGAAACAACTCA 639 525 229 Antisense
AACTCATTTATTGCAAATTATCCCG 296 141 248 Antisense
TTGCAAATTATCCCGTATTCCACTA 593 701 258 Antisense
Sequence Source Affymetrix Proprietary Database