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[173083_s_at] calcium-binding protein

Description AV199772 /REP_DB=TREMBL Accession /FEA=EST Transcript /GB=AV199772 /DEF=Caenorhabditis elegans cDNA clone:yk551e4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 80      6grp_highest_nonorm > 54      6grp_total > 82      4grp_total > 57      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2300
1 2 3 4 range
A 2.6
(-3)
A 		1657.9
(1.6)
P 		2175.3
(3)
P 		30.1
(-2.9)
A 		2173
B 2.5
(-3)
A 		1818.2
(1.7)
P 		2302
(3)
P 		13.3
(-3)
A 		2300
C 3.5
(-3)
A 		1382.6
(0.8)
P 		2203.7
(3)
P 		9.8
(-3)
A 		2200
avr 2.9
(-3) 		1619.6
(1.4) 		2227
(3) 		17.7
(-3) 		2224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2037
1 2 3 4 5 6 range
A 95.4
(-3)
P 		1464.7
(1.3)
P 		1373.2
(1)
P 		2012.8
(3)
P 		1360.2
(1)
P 		612.3
(-1.4)
P 		1917
B 80.1
(-3)
P 		1587.3
(1.4)
P 		1082.8
(-0)
P 		1311.4
(0.6)
P 		2117.2
(3)
P 		667
(-1.3)
P 		2037
C 81.8
(-3)
P 		1567.6
(3)
P 		1098.8
(1.1)
P 		1366.4
(2.2)
P 		1508.7
(2.8)
P 		842.3
(0.1)
P 		1486
avr 85.8
(-3) 		1539.9
(2.5) 		1184.9
(1.2) 		1563.5
(2.6) 		1662
(3) 		707.2
(-0.6) 		1576
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 767
1 2 3 4 range
A 0.9
(-3)
A 		359.9
(0.8)
P 		568.7
(3)
P 		4.4
(-3)
A 		568
B 0.8
(-3)
A 		449.1
(2.1)
P 		533.4
(3)
P 		2.8
(-3)
A 		533
C 1.2
(-3)
A 		397.5
(0.1)
P 		768.1
(3)
P 		1.7
(-3)
A 		767
avr 1
(-3) 		402.2
(0.9) 		623.4
(3) 		3
(-3) 		622

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 843
1 2 3 4 5 6 range
A 17.6
(-3)
P 		520.9
(3)
P 		299.1
(0.4)
P 		510.5
(2.9)
P 		410.4
(1.7)
P 		149.1
(-1.4)
P 		503
B 27.9
(-3)
P 		528.3
(0.6)
P 		262.9
(-1.3)
P 		347.3
(-0.7)
P 		860.2
(3)
P 		248.7
(-1.4)
P 		832
C 30.3
(-3)
P 		478.3
(2.9)
P 		279.7
(0.3)
P 		274.8
(0.2)
P 		488.2
(3)
P 		282
(0.3)
P 		458
avr 25.3
(-3) 		509.2
(2.2) 		280.6
(-0.3) 		377.5
(0.8) 		586.3
(3) 		226.6
(-0.8) 		561
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173083_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28268
Sequence Type Exemplar sequence
Representative Public ID AV199772 NCBI
Target Description AV199772 /REP_DB=TREMBL Accession /FEA=EST Transcript /GB=AV199772 /DEF=Caenorhabditis elegans cDNA clone:yk551e4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064727(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064727 NCBI Caenorhabditis elegans H10E21.4 (H10E21.4) mRNA, complete cds. 11/11 A 
SNAP00000020263 ENSEMBL cdna:SNAP chromosome:CEL140:III:15499:19563:-1 11/11 None
GENEFINDER00000020266 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:17421:19563:-1 11/11 None
H10E21.4 ENSEMBL cdna:known chromosome:CEL140:III:17383:19601:-1 gene:H10E21.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:18736-19523(-) 91.33 91.33
Public Domain and Genome References
Gene Title calcium-binding protein
Ensembl H10E21.4
Entrez Gene 175168 Entrez gene
SwissProt O76670 EMBL-EBI
WormBase CE19489 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC26922 Hypothetical protein H10E21.4 [Caenorhabditis elegans] ref|NP_497128.1| H10E21.4 [Caenorhabditis elegans] 1.0E-130
blast AAC26922 Hypothetical protein H10E21.4 [Caenorhabditis elegans] ref|NP_497128.1| H10E21.4 [Caenorhabditis elegans] 1.0E-129
blast CAE69167 Hypothetical protein CBG15199 [Caenorhabditis briggsae] 1.0E-111
blast CAE69167 Hypothetical protein CBG15199 [Caenorhabditis briggsae] 1.0E-110
blast ABA94450 calmodulin [Oryza sativa (japonica cultivar-group)] 8.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 7.5E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 7.0E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.71
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 7.6E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.2
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.4E-7
Sequence
Target Sequence 
>C. ELEGANS:173083_S_AT
gaatgactcattcgaccttgtggatgcgaacaatgatggaaagctgtccganaangaact
aatcacttttatgatgaatcgangaatgcctgatgggcaanaatacttccaacgcttcga
tttggaccgtaacgggcacttggatatttctgagtttgtgccattggtctatga

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGACTCATTCGACCTTGTGGAT 251 331 29 Antisense
GACTCATTCGACCTTGTGGATGCGA 3 373 33 Antisense
TCGACCTTGTGGATGCGAACAATGA 478 581 40 Antisense
CGAACAATGATGGAAAGCTGTCCGA 109 253 55 Antisense
AATACTTCCAACGCTTCGATTTGGA 12 171 130 Antisense
CAACGCTTCGATTTGGACCGTAACG 61 187 138 Antisense
GCTTCGATTTGGACCGTAACGGGCA 394 305 142 Antisense
GATTTGGACCGTAACGGGCACTTGG 447 293 147 Antisense
ACGGGCACTTGGATATTTCTGAGTT 141 95 160 Antisense
ATTTCTGAGTTTGTGCCATTGGTCT 203 17 174 Antisense
CTGAGTTTGTGCCATTGGTCTATGA 72 237 178 Antisense
Sequence Source Affymetrix Proprietary Database