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[173070_s_at] mitotic chromosome and X-chromosome associated MIX-1 protein

Description AV183196_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV183196 /DEF=Caenorhabditis elegans cDNA clone:yk647g6 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 47      6grp_total > 47      4grp_total > 42      6grp_cgc > 16      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 160.6
(-2.8)
P 		197
(1.7)
P 		207
(3)
M 		159.1
(-3)
A 		48
B 132.4
(-0.4)
P 		159.3
(3)
A 		111.5
(-3)
A 		122.2
(-1.7)
A 		48
C 175.5
(-3)
P 		239.2
(3)
P 		219.5
(1.1)
P 		233.4
(2.5)
A 		64
avr 156.2
(-3) 		198.5
(3) 		179.3
(0.3) 		171.6
(-0.8) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 240.1
(-0)
M 		296.8
(1.7)
P 		161.8
(-2.5)
P 		195.4
(-1.4)
P 		144.7
(-3)
P 		338.6
(3)
P 		194
B 252.1
(2.3)
P 		199.3
(-0.6)
P 		154.5
(-3)
P 		156.1
(-2.9)
P 		195.6
(-0.8)
P 		265.3
(3)
P 		111
C 312.1
(3)
P 		252
(-0.2)
P 		226.7
(-1.5)
P 		262.4
(0.4)
P 		199.4
(-3)
P 		208.7
(-2.5)
P 		113
avr 268.1
(2.8) 		249.4
(1.6) 		181
(-2.9) 		204.6
(-1.4) 		179.9
(-3) 		270.9
(3) 		91
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 58.5
(3)
P 		42.8
(0.3)
P 		54.1
(2.3)
M 		23.3
(-3)
A 		35
B 42.2
(3)
P 		39.3
(1.9)
A 		25.8
(-3)
A 		26
(-2.9)
A 		16
C 58.1
(0)
P 		68.8
(1.8)
P 		76.5
(3)
P 		39.4
(-3)
A 		37
avr 52.9
(3) 		50.3
(2.3) 		52.1
(2.8) 		29.6
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 44.3
(-2.2)
M 		105.6
(3)
P 		35.2
(-3)
P 		49.6
(-1.8)
P 		43.7
(-2.3)
P 		82.4
(1)
P 		70
B 87.7
(1.9)
P 		66.4
(-0.2)
P 		37.5
(-3)
P 		41.3
(-2.6)
P 		79.5
(1.1)
P 		98.9
(3)
P 		61
C 115.7
(3)
P 		76.9
(-0.7)
P 		57.7
(-2.5)
P 		52.8
(-3)
P 		64.5
(-1.9)
P 		69.9
(-1.4)
P 		63
avr 82.6
(2.8) 		83
(2.9) 		43.5
(-3) 		47.9
(-2.3) 		62.6
(-0.1) 		83.7
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173070_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26850
Sequence Type Exemplar sequence
Representative Public ID AV183196 NCBI
Target Description AV183196_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV183196 /DEF=Caenorhabditis elegans cDNA clone:yk647g6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063930(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063930 NCBI Caenorhabditis elegans MItosis and X associated family member (mix-1) (mix-1) mRNA, complete cds. 11/11 A 
SNAP00000018716 ENSEMBL cdna:SNAP chromosome:CEL140:II:10836243:10841131:1 11/11 None
GENEFINDER00000018728 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10835695:10841131:1 11/11 None
M106.1 ENSEMBL cdna:known chromosome:CEL140:II:10824206:10841319:1 gene:M106.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10840925-10841170(+) 80.67 80.67
Public Domain and Genome References
Gene Title mitotic chromosome and X-chromosome associated MIX-1 protein
Gene Symbol mix-1
Ensembl M106.1
Entrez Gene 174669 Entrez gene
SwissProt Q09591 EMBL-EBI
WormBase CE18083 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
7059 chromosome segregation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
793 condensed chromosome inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87054 Hypothetical protein M106.1 [Caenorhabditis elegans] emb|CAA86786.1| Hypothetical protein M106.1 [Caenorhabditis elegans] emb|CAA20330.1| Hypothetical protein M106.1 [Caenorhabditis elegans] ref|NP_496331.1| MItosis and X associated family member (mix-1) [Caenorhabditis elegans] sp|Q09591|MIX1_CAEEL Mitotic chromosome and X-chromosome associated protein mix-1 (Structural maintenance of chromosome 2) (Protein let-29) gb|AAC47834.1| mitotic chromosome and X-chromosome associated MIX-1 protein [Caenorhabditis elegans] 0.0
blast CAE59599 Hypothetical protein CBG03006 [Caenorhabditis briggsae] 0.0
blast T23981 hypothetical protein R06F6.10 - Caenorhabditis elegans 0.0
blast CAE59599 Hypothetical protein CBG03006 [Caenorhabditis briggsae] 1.0E-173
Protein Domains
Database ID Description E-Value
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 5.59999990463257
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 4.09999990463257
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 2.0
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 4.09999990463257
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 2.70000004768372
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 1.0E-19
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 2.4E-20
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR003395 EMBL-EBI SMC protein, N-terminal 4.6E-47
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 9.5E-38
Sequence
Target Sequence 
>C. ELEGANS:173070_S_AT
ggacattctacatgtacacgtctcaatggaggagatattgcagtactttgccaggataaa
gttctacaggcacaagctcttgagctcacagatgctggaaaagcaaagaaggacgccgcc
gccaagaagggagctcagaagaatgacaaagaacctccaaagaagaagcctattgtcgtc
gacgacgatgacttcgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACATTCTACATGTACACGTCTCA 531 525 15 Antisense
TACATGTACACGTCTCAATGGAGGA 196 645 23 Antisense
GATATTGCAGTACTTTGCCAGGATA 311 427 48 Antisense
AAAGTTCTACAGGCACAAGCTCTTG 646 121 72 Antisense
GGCACAAGCTCTTGAGCTCACAGAT 419 535 83 Antisense
GAGCTCACAGATGCTGGAAAAGCAA 434 387 96 Antisense
CCGCCGCCAAGAAGGGAGCTCAGAA 27 263 130 Antisense
GAAGAATGACAAAGAACCTCCAAAG 518 339 152 Antisense
AGAACCTCCAAAGAAGAAGCCTATT 150 67 164 Antisense
AAGCCTATTGTCGTCGACGACGATG 386 149 180 Antisense
GTCGTCGACGACGATGACTTCGAGT 128 473 189 Antisense
Sequence Source Affymetrix Proprietary Database