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[173060_s_at]

Description C58103_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C58103 /DEF=C.elegans cDNA clone yk361c12 : 3prime end, single read.
Cluster 6grp_total > 98      4grp_total > 12      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 19.8
(-0)
A 		2.1
(-3)
A 		4.7
(-2.6)
A 		37.6
(3)
A 		36
B 14.2
(3)
A 		5.5
(-1.2)
A 		3.3
(-2.3)
A 		1.9
(-3)
A 		12
C 3.2
(-3)
A 		16.7
(-1.6)
A 		3.7
(-2.9)
A 		61.8
(3)
A 		59
avr 12.4
(-1.3) 		8.1
(-2.2) 		3.9
(-3) 		33.8
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 405
1 2 3 4 5 6 range
A 2.8
(-3)
A 		12
(-2.8)
A 		7
(-2.9)
A 		15.8
(-2.8)
A 		315.9
(2.2)
P 		362.3
(3)
P 		360
B 3.9
(-3)
A 		5.2
(-3)
A 		6.5
(-3)
A 		31.6
(-2.5)
A 		285
(1.8)
P 		357.4
(3)
P 		354
C 15.9
(-2.8)
A 		2.7
(-3)
A 		6.1
(-2.9)
A 		41.8
(-2.4)
A 		273.3
(1)
P 		408
(3)
P 		405
avr 7.5
(-3) 		6.6
(-3) 		6.5
(-3) 		29.7
(-2.6) 		291.4
(1.6) 		375.9
(3) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 7.2
(3)
A 		0.5
(-3)
A 		1.2
(-2.4)
A 		5.5
(1.5)
A 		7
B 4.5
(3)
A 		1.4
(-1.5)
A 		0.8
(-2.4)
A 		0.4
(-3)
A 		4
C 1.1
(-3)
A 		4.8
(-0.6)
A 		1.3
(-2.9)
A 		10.4
(3)
A 		9
avr 4.3
(1.5) 		2.2
(-1.5) 		1.1
(-3) 		5.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 0.5
(-3)
A 		4.3
(-2.8)
A 		1.5
(-2.9)
A 		4
(-2.8)
A 		95.3
(3)
P 		88.2
(2.6)
P 		95
B 1.4
(-3)
A 		1.7
(-3)
A 		1.6
(-3)
A 		8.4
(-2.7)
A 		115.8
(2.2)
P 		133.3
(3)
P 		132
C 5.9
(-2.8)
A 		0.8
(-3)
A 		1.5
(-3)
A 		8.4
(-2.7)
A 		88.4
(0.9)
P 		136.6
(3)
P 		136
avr 2.6
(-2.9) 		2.3
(-3) 		1.5
(-3) 		6.9
(-2.7) 		99.8
(2) 		119.4
(3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173060_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26604
Sequence Type Exemplar sequence
Representative Public ID C58103 NCBI
Target Description C58103_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C58103 /DEF=C.elegans cDNA clone yk361c12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068723(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068723 NCBI Caenorhabditis elegans C34D4.3 (C34D4.3) mRNA, complete cds. 11/11 A 
SNAP00000003806 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7153650:7154094:1 9/11 None
GENEFINDER00000003814 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7153650:7154090:1 11/11 None
C34D4.3 ENSEMBL cdna:known chromosome:CEL140:IV:7153645:7154166:1 gene:C34D4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7153828-7154117(+) 80.33 80.33
Public Domain and Genome References
Gene Title
Ensembl C34D4.3
Entrez Gene 177488 Entrez gene
SwissProt Q18451 EMBL-EBI
WormBase CE06924 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00703 Hypothetical protein C34D4.3 [Caenorhabditis elegans] ref|NP_501124.1| C34D4.3 [Caenorhabditis elegans] 1.0E-59
blast CAA87431 Hypothetical protein ZK1307.3 [Caenorhabditis elegans] ref|NP_496079.1| ZK1307.3 [Caenorhabditis elegans] sp|Q09361|YS13_CAEEL Hypothetical protein ZK1307.3 in chromosome II 8.0E-26
blast CAA87432 Hypothetical protein ZK1307.4 [Caenorhabditis elegans] ref|NP_496078.1| ZK1307.4 [Caenorhabditis elegans] sp|Q09362|YS14_CAEEL Hypothetical protein ZK1307.4 in chromosome II 2.0E-23
blast T27729 hypothetical protein ZK1307.4 - Caenorhabditis elegans 9.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 2.4E-29
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 8.9E-7
Sequence
Target Sequence 
>C. ELEGANS:173060_S_AT
ttgggtataccgctgatctgatcatcactagaaagccaggaaagccgcaggccgacaagc
ttgttatccaattcgctgccgttgagcagacttgccgtgatggctgtatttgccaaggct
gtatttgccaatggactgccaactggagagtgctgtggagagacgatcatcaagctgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGGTATACCGCTGATCTGATCAT 542 689 104 Antisense
TGATCATCACTAGAAAGCCAGGAAA 689 563 122 Antisense
CAGGCCGACAAGCTTGTTATCCAAT 187 209 151 Antisense
TTATCCAATTCGCTGCCGTTGAGCA 56 681 167 Antisense
TGCCGTTGAGCAGACTTGCCGTGAT 221 585 180 Antisense
GACTTGCCGTGATGGCTGTATTTGC 630 371 192 Antisense
GTATTTGCCAAGGCTGTATTTGCCA 639 453 209 Antisense
GTATTTGCCAATGGACTGCCAACTG 357 453 224 Antisense
GGACTGCCAACTGGAGAGTGCTGTG 175 523 236 Antisense
ACTGGAGAGTGCTGTGGAGAGACGA 142 97 245 Antisense
GTGGAGAGACGATCATCAAGCTGTC 97 491 258 Antisense
Sequence Source Affymetrix Proprietary Database