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[173055_s_at] heat shock protein (HSP90)

Description C52030_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52030 /DEF=C.elegans cDNA clone yk224h12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 46      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 28      6grp_total > 45      4grp_total > 01      6grp_highest_sub > Cu      6grp_cgc > 45      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25414
1 2 3 4 range
A 10322.9
(-2.9)
P 		10079.3
(-3)
P 		11493
(-2.6)
P 		32630.5
(3)
P 		22551
B 9679.4
(-3)
P 		9709.7
(-3)
P 		11508.6
(-2.4)
P 		29555.1
(3)
P 		19876
C 9656.5
(-3)
P 		9506.3
(-3)
P 		10257.9
(-2.8)
P 		34920
(3)
P 		25414
avr 9886.3
(-3) 		9765.1
(-3) 		11086.5
(-2.6) 		32368.5
(3) 		22603

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3253
1 2 3 4 5 6 range
A 11113.4
(3)
P 		9853.5
(0.4)
P 		10805.6
(2.4)
P 		8235.8
(-3)
P 		8478.7
(-2.5)
P 		10256.5
(1.2)
P 		2878
B 11001
(2)
P 		11017.1
(2)
P 		11488.4
(3)
P 		9626.8
(-0.8)
P 		8553.6
(-3)
P 		10738
(1.5)
P 		2935
C 9145.2
(-3)
P 		11043.5
(2.5)
P 		9416.3
(-2.2)
P 		9196.3
(-2.9)
P 		9316.7
(-2.5)
P 		11231.2
(3)
P 		2086
avr 10419.9
(2) 		10638
(2.7) 		10570.1
(2.5) 		9019.6
(-2.3) 		8783
(-3) 		10741.9
(3) 		1959
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4110
1 2 3 4 range
A 3758.6
(0.6)
P 		2188
(-3)
P 		3004.5
(-1.1)
P 		4776.5
(3)
P 		2589
B 3086.7
(-1.9)
P 		2398.1
(-3)
P 		2666.8
(-2.6)
P 		6298.2
(3)
P 		3900
C 3196.6
(-2.1)
P 		2733.1
(-3)
P 		3575.2
(-1.4)
P 		5888.1
(3)
P 		3155
avr 3347.3
(-1.3) 		2439.7
(-3) 		3082.2
(-1.8) 		5654.3
(3) 		3215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2155
1 2 3 4 5 6 range
A 2048.7
(-3)
P 		3504.4
(3)
P 		2353.2
(-1.7)
P 		2088.8
(-2.8)
P 		2558.5
(-0.9)
P 		2497.1
(-1.2)
P 		1456
B 3827.8
(2.3)
P 		3667.1
(1.6)
P 		2789.7
(-2)
P 		2549.8
(-3)
P 		3475.2
(0.8)
P 		4004.2
(3)
P 		1454
C 3389.7
(1.8)
P 		3369.7
(1.8)
P 		2396.6
(-1.3)
P 		1849.4
(-3)
P 		3014.7
(0.7)
P 		3759.7
(3)
P 		1910
avr 3088.7
(1.1) 		3513.7
(3) 		2513.2
(-1.4) 		2162.7
(-3) 		3016.1
(0.8) 		3420.3
(2.6) 		1351
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173055_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26447
Sequence Type Exemplar sequence
Representative Public ID C52030 NCBI
Target Description C52030_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52030 /DEF=C.elegans cDNA clone yk224h12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074225 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-21) (daf-21) mRNA, complete cds. 11/11 A 
SNAP00000005835 ENSEMBL cdna:SNAP chromosome:CEL140:V:14688668:14691014:1 11/11 None
GENEFINDER00000005847 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14688753:14691014:1 11/11 None
C47E8.5.2 ENSEMBL cdna:known chromosome:CEL140:V:14687507:14691132:1 gene:C47E8.5 11/11 A 
C47E8.5.3 ENSEMBL cdna:known chromosome:CEL140:V:14688474:14691132:1 gene:C47E8.5 11/11 A 
C47E8.5.4 ENSEMBL cdna:known chromosome:CEL140:V:14688747:14691132:1 gene:C47E8.5 11/11 None
C47E8.5.1 ENSEMBL cdna:known chromosome:CEL140:V:14688749:14691132:1 gene:C47E8.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14690757-14691010(+) 84.0 84.0
Public Domain and Genome References
Gene Title heat shock protein (HSP90)
Gene Symbol daf-21
Ensembl C47E8.5
Entrez Gene 179971 Entrez gene
SwissProt Q18688 EMBL-EBI
WormBase CE05441 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248045_AT heat shock protein 81-2 (HSP81-2) at
CHICKEN:GGA.4332.1.S1_AT heat shock 90kDa protein 1, beta gga
PLASMODIUM_ANOPHELES:200064_AT heat shock 90kDa protein 1, beta hs
HG-U133B:200064_AT heat shock 90kDa protein 1, beta hs
HG-U133_PLUS_2:200064_AT heat shock 90kDa protein 1, beta hs
HG-U133A_2:200064_AT heat shock 90kDa protein 1, beta hs
HG-U133A:200064_AT heat shock 90kDa protein 1, beta hs
HG-FOCUS:200064_AT heat shock 90kDa protein 1, beta hs
U133_X3P:200064_AT heat shock 90kDa protein 1, beta hs
HG-U95AV2:1161_AT heat shock 90kDa protein 1, beta hs
HC-G110:1161_AT heat shock 90kDa protein 1, beta hs
HG-U95AV2:33984_AT heat shock 90kDa protein 1, beta hs
HUGENEFL:J04988_AT heat shock 90kDa protein 1, beta hs
U133_X3P:G9082288_3P_AT heat shock 90kDa protein 1, beta hs
U133_X3P:HS2.434250.1.S1_3P_S_AT heat shock 90kDa protein 1, beta hs
U133_X3P:HS.74335.1.S1_3P_A_AT heat shock 90kDa protein 1, beta hs
HG-U133_PLUS_2:1557910_AT heat shock 90kDa protein 1, beta hs
HG-U133A_2:214359_S_AT heat shock 90kDa protein 1, beta hs
HG-FOCUS:214359_S_AT heat shock 90kDa protein 1, beta hs
HG-U133_PLUS_2:214359_S_AT heat shock 90kDa protein 1, beta hs
HG-U133A:214359_S_AT heat shock 90kDa protein 1, beta hs
HG-U95AV2:33985_S_AT Heat shock 90kDa protein 1, beta hs
HG-U95AV2:33986_R_AT Heat shock 90kDa protein 1, beta hs
MOE430A:1416364_AT heat shock protein 1, beta mm
MOUSE430_2:1416364_AT heat shock protein 1, beta mm
MOUSE430A_2:1416364_AT heat shock protein 1, beta mm
MOE430A:1416365_AT heat shock protein 1, beta mm
MOUSE430A_2:1416365_AT heat shock protein 1, beta mm
MOUSE430_2:1416365_AT heat shock protein 1, beta mm
MU11KSUBA:M18186_F_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.14391.0_F_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.34568.0_F_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.32496.0_F_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.31698.0_F_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.777.0_F_AT heat shock protein 1, beta mm
MG-U74AV2:95359_AT heat shock protein 1, beta mm
MU11KSUBB:MSA.41809.0_F_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBB:TC27126_F_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBB:TC27127_F_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBB:TC27127_I_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBB:TC27128_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBB:TC27129_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBC:TC41795_F_AT Heat shock protein 1, beta (Hspcb), mRNA mm
MU19KSUBC:TC41795_I_AT Heat shock protein 1, beta (Hspcb), mRNA mm
RN-U34:S45392_AT heat shock 90kDa protein 1, beta rn
RT-U34:S45392_AT heat shock 90kDa protein 1, beta rn
RG-U34A:S45392_AT heat shock 90kDa protein 1, beta rn
RT-U34:RC_AI008074_S_AT heat shock 90kDa protein 1, beta rn
RG-U34A:RC_AI008074_S_AT heat shock 90kDa protein 1, beta rn
RT-U34:RC_AI007820_S_AT heat shock 90kDa protein 1, beta rn
RG-U34A:RC_AI007820_S_AT heat shock 90kDa protein 1, beta rn
RT-U34:RC_AI236795_S_AT heat shock 90kDa protein 1, beta rn
RG-U34A:RC_AI236795_S_AT heat shock 90kDa protein 1, beta rn
RAE230A:1375336_AT heat shock 90kDa protein 1, beta rn
RAT230_2:1375336_AT heat shock 90kDa protein 1, beta rn
RAE230A:1375335_AT heat shock 90kDa protein 1, beta rn
RAT230_2:1375335_AT heat shock 90kDa protein 1, beta rn
RAE230B:1393555_AT heat shock 90kDa protein 1, beta rn
RAT230_2:1393555_AT heat shock 90kDa protein 1, beta rn
YEAST_2:1775624_AT Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA99793 Hypothetical protein C47E8.5 [Caenorhabditis elegans] ref|NP_506626.1| abnormal DAuer Formation family member (daf-21) [Caenorhabditis elegans] 0.0
blast CAE60851 Hypothetical protein CBG04560 [Caenorhabditis briggsae] 0.0
blast CAA06695 heat shock protein 90 [Brugia pahangi] sp|O61998|HSP90_BRUPA Heat shock protein 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001404 EMBL-EBI Heat shock protein Hsp90 1.0E-126
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 9.4E-17
Pfam IPR001404 EMBL-EBI Heat shock protein Hsp90 1.0E-126
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 9.4E-17
Sequence
Target Sequence 
>C. ELEGANS:173055_S_AT
gtcgaggtcgataagaatgacaagaccgataaggattnggtngttnttcttntcgagact
gctcttntcgcttccggattctcccttgaggagccacaatctcacgcttcccgcatntac
agaatgatcaagcttggtctcgatatcggagatgacgaaattgaagattctgctgttcca
tcatcgtgcaccgctgaggccaagattgagggagctgaggaggatgcttcccgc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGAGGTCGATAAGAATGACAAGA 351 473 18 Antisense
TTCCGGATTCTCCCTTGAGGAGCCA 505 695 89 Antisense
GATTCTCCCTTGAGGAGCCACAATC 621 427 94 Antisense
CTTGAGGAGCCACAATCTCACGCTT 256 217 102 Antisense
ACAGAATGATCAAGCTTGGTCTCGA 538 107 136 Antisense
GATCAAGCTTGGTCTCGATATCGGA 637 421 143 Antisense
GCTTGGTCTCGATATCGGAGATGAC 356 307 149 Antisense
TGAAGATTCTGCTGTTCCATCATCG 198 575 179 Antisense
TGCACCGCTGAGGCCAAGATTGAGG 539 577 204 Antisense
AGGCCAAGATTGAGGGAGCTGAGGA 503 53 214 Antisense
GGGAGCTGAGGAGGATGCTTCCCGC 128 495 227 Antisense
Sequence Source Affymetrix Proprietary Database