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[173053_s_at] man(9)-alpha-mannosidase

Description C35670_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C35670 /DEF=C.elegans cDNA clone yk436e10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 42      6grp_total > 22      4grp_total > 07      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 304
1 2 3 4 range
A 910.5
(3)
P 		650.2
(-3)
P 		810.5
(0.7)
P 		743.3
(-0.9)
P 		260
B 863.2
(1.8)
P 		622.3
(-3)
P 		741.8
(-0.6)
P 		926.4
(3)
P 		304
C 897.6
(3)
P 		699.3
(-3)
P 		864.3
(2)
P 		882.6
(2.5)
P 		198
avr 890.4
(3) 		657.3
(-3) 		805.5
(0.8) 		850.8
(2) 		233

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 907.7
(2.3)
P 		943.9
(3)
P 		819.7
(0.7)
P 		774.4
(-0.2)
P 		751.6
(-0.6)
P 		625.5
(-3)
P 		318
B 963.8
(1.7)
P 		972.7
(1.9)
P 		1024.4
(3)
P 		858.2
(-0.6)
P 		775
(-2.4)
P 		746.6
(-3)
P 		278
C 871.1
(0.4)
P 		1012.6
(3)
P 		880.6
(0.6)
P 		761.7
(-1.7)
P 		708.4
(-2.6)
P 		689
(-3)
P 		324
avr 914.2
(1.7) 		976.4
(3) 		908.2
(1.6) 		798.1
(-0.7) 		745
(-1.8) 		687
(-3) 		289
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 331.5
(3)
P 		141.1
(-2.1)
P 		211.9
(-0.2)
P 		108.8
(-3)
P 		223
B 275.3
(3)
P 		153.7
(-3)
P 		171.9
(-2.1)
P 		197.4
(-0.8)
P 		122
C 297.1
(2.8)
P 		201
(-0.9)
P 		301.2
(3)
P 		148.8
(-3)
P 		152
avr 301.3
(3) 		165.3
(-2.5) 		228.3
(0.1) 		151.7
(-3) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 167.3
(-2.5)
P 		335.7
(3)
P 		178.5
(-2.1)
P 		196.4
(-1.6)
P 		226.8
(-0.6)
P 		152.3
(-3)
P 		183
B 335.3
(3)
P 		323.8
(2.4)
P 		248.8
(-1.8)
P 		227.3
(-3)
P 		314.9
(1.9)
P 		278.4
(-0.2)
P 		108
C 322.9
(3)
P 		309
(2.5)
P 		224.1
(-0.5)
P 		153.2
(-3)
P 		229.2
(-0.3)
P 		230.6
(-0.3)
P 		170
avr 275.2
(0.8) 		322.8
(3) 		217.1
(-1.9) 		192.3
(-3) 		257
(-0) 		220.4
(-1.7) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173053_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26370
Sequence Type Exemplar sequence
Representative Public ID C35670 NCBI
Target Description C35670_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C35670 /DEF=C.elegans cDNA clone yk436e10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077270(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077270 NCBI Caenorhabditis elegans ZC506.1 (man(9)-alpha-mannosidase) mRNA, complete cds. 11/11 A 
GENEFINDER00000023348 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9958966:9964246:1 11/11 None
SNAP00000023344 ENSEMBL cdna:SNAP chromosome:CEL140:X:9962523:9964246:1 11/11 None
ZC506.1 ENSEMBL cdna:known chromosome:CEL140:X:9958892:9964665:1 gene:ZC506.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9963370-9964131(+) 84.33 84.33
Public Domain and Genome References
Gene Title man(9)-alpha-mannosidase
Ensembl ZC506.1
Entrez Gene 181210 Entrez gene
SwissProt Q09641 EMBL-EBI
WormBase CE37461 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.20827.1.S1_AT similar to Putative alpha-mannosidase C1orf22 cfa
DROSOPHILA_2:1630203_AT dm
DROSGENOME1:146315_AT dm
CHICKEN:GGA.10298.1.S1_S_AT similar to Putative alpha-mannosidase C1orf22 gga
CHICKEN:GGAAFFX.26310.1.S1_AT similar to Putative alpha-mannosidase C1orf22 gga
HG-U133_PLUS_2:220342_X_AT chromosome 1 open reading frame 22 hs
HG-U133A:220342_X_AT chromosome 1 open reading frame 22 hs
HG-U133A_2:220342_X_AT chromosome 1 open reading frame 22 hs
HU35KSUBA:RC_AA256323_AT chromosome 1 open reading frame 22 hs
HU35KSUBB:RC_AA179387_AT chromosome 1 open reading frame 22 hs
HG-U133A:220926_S_AT chromosome 1 open reading frame 22 hs
HG-U133_PLUS_2:220926_S_AT chromosome 1 open reading frame 22 hs
HG-U133A_2:220926_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:G8922223_3P_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:G13376781_3P_S_AT chromosome 1 open reading frame 22 hs
U133_X3P:HS.301997.1.S1_3P_S_AT chromosome 1 open reading frame 22 hs
HG-U133_PLUS_2:223243_S_AT chromosome 1 open reading frame 22 hs
HG-U133B:223243_S_AT chromosome 1 open reading frame 22 hs
HG-U95B:59633_AT chromosome 1 open reading frame 22 hs
HG-U95C:65608_AT chromosome 1 open reading frame 22 hs
HG-U95E:90857_AT chromosome 1 open reading frame 22 hs
HG-U95C:56528_R_AT Chromosome 1 open reading frame 22 hs
MG-U74AV2:104114_AT RIKEN cDNA 2310050N11 gene mm
MOE430A:1460401_AT RIKEN cDNA 2310050N11 gene mm
MOUSE430A_2:1460401_AT RIKEN cDNA 2310050N11 gene mm
MOUSE430_2:1460401_AT RIKEN cDNA 2310050N11 gene mm
MG-U74BV2:105544_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MG-U74BV2:110546_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MOE430B:1459133_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
MOUSE430_2:1459133_AT RIKEN cDNA 2310050N11 gene, mRNA (cDNA clone IMAGE:3984921) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87371 Hypothetical protein ZC506.1 [Caenorhabditis elegans] ref|NP_509671.3| ZC506.1 [Caenorhabditis elegans] 0.0
blast CAE58760 Hypothetical protein CBG01952 [Caenorhabditis briggsae] 0.0
blast XP_193956 PREDICTED: hypothetical protein LOC66967 [Mus musculus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Pfam IPR003137 EMBL-EBI Protease-associated PA 1.6E-29
Pfam IPR003137 EMBL-EBI Protease-associated PA 1.6E-29
Sequence
Target Sequence 
>C. ELEGANS:173053_S_AT
gatagtgatccaagactacatcaagaaattgttgagcgtcacgtcacagaactacaaaaa
tatctagagttcaaggatgctgactatgcgttccagttttatgagttcttccgtacggca
gcatatggagcacttgatttgaatgtggaaaatagtaagctgctcacagtaatttctgca
ctgagtacgttaaaaacaaaaccaattccaccatcagtttttgcgagacttcccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAGTGATCCAAGACTACATCAAG 373 425 31 Antisense
TTGTTGAGCGTCACGTCACAGAACT 262 699 59 Antisense
GAGCGTCACGTCACAGAACTACAAA 356 387 64 Antisense
AAGGATGCTGACTATGCGTTCCAGT 614 159 103 Antisense
TGCGTTCCAGTTTTATGAGTTCTTC 602 583 117 Antisense
TGAGTTCTTCCGTACGGCAGCATAT 99 567 132 Antisense
GGCAGCATATGGAGCACTTGATTTG 326 535 147 Antisense
AGCTGCTCACAGTAATTTCTGCACT 475 81 188 Antisense
GTAATTTCTGCACTGAGTACGTTAA 435 463 199 Antisense
ATTCCACCATCAGTTTTTGCGAGAC 404 7 235 Antisense
CCATCAGTTTTTGCGAGACTTCCCG 324 271 241 Antisense
Sequence Source Affymetrix Proprietary Database