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[173052_at]

Description C35214_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C35214 /DEF=C.elegans cDNA clone yk426f12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 79      6grp_total > 78      4grp_total > 02      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2483
1 2 3 4 range
A 1181.1
(-2.8)
P 		1253.1
(-2.6)
P 		1098.3
(-3)
P 		3278.4
(3)
P 		2180
B 1274.2
(-2.4)
P 		1035.2
(-3)
P 		1016
(-3)
P 		3499.2
(3)
P 		2483
C 1167.8
(-2.8)
P 		1228
(-2.6)
P 		1093.3
(-3)
P 		2956.3
(3)
P 		1863
avr 1207.7
(-2.6) 		1172.1
(-2.7) 		1069.2
(-3) 		3244.6
(3) 		2175

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1944
1 2 3 4 5 6 range
A 1710.3
(-1.2)
P 		1157.2
(-2.9)
P 		1126.8
(-3)
P 		1362.4
(-2.3)
P 		1945.5
(-0.5)
P 		3054.5
(3)
P 		1928
B 1601.7
(-1.6)
P 		1255.4
(-3)
P 		1272.6
(-2.9)
P 		1457.3
(-2.2)
P 		1838
(-0.6)
P 		2689.1
(3)
P 		1434
C 1688.8
(-0.8)
P 		1110.2
(-3)
P 		1161.4
(-2.8)
P 		1436.5
(-1.8)
P 		2109.4
(0.8)
P 		2693.2
(3)
P 		1583
avr 1666.9
(-1.2) 		1174.3
(-3) 		1186.9
(-3) 		1418.7
(-2.1) 		1964.3
(-0.1) 		2812.3
(3) 		1638
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 510
1 2 3 4 range
A 430.1
(1.6)
P 		272
(-3)
P 		287.1
(-2.6)
P 		479.9
(3)
P 		208
B 406.3
(-1)
P 		255.7
(-2.8)
P 		235.4
(-3)
P 		745.7
(3)
P 		510
C 386.6
(-1.6)
P 		353.1
(-3)
P 		381
(-1.8)
P 		498.5
(3)
P 		145
avr 407.7
(-0.6) 		293.6
(-3) 		301.2
(-2.8) 		574.7
(3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 757
1 2 3 4 5 6 range
A 315.3
(-2.2)
P 		411.6
(-1)
P 		245.4
(-3)
P 		345.5
(-1.8)
P 		587.1
(1.1)
P 		743.7
(3)
P 		498
B 557.3
(-0.9)
P 		417.9
(-2.1)
P 		309
(-3)
P 		386
(-2.3)
P 		746.8
(0.8)
P 		1002.7
(3)
P 		694
C 626
(0.3)
P 		338.8
(-2.5)
P 		295.6
(-2.9)
P 		288.9
(-3)
P 		682.6
(0.9)
P 		901.6
(3)
P 		613
avr 499.5
(-0.8) 		389.4
(-1.9) 		283.3
(-3) 		340.1
(-2.4) 		672.2
(0.9) 		882.7
(3) 		599
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173052_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26358
Sequence Type Exemplar sequence
Representative Public ID C35214 NCBI
Target Description C35214_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C35214 /DEF=C.elegans cDNA clone yk426f12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060363(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060363 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-25) (ubc-25) mRNA, complete cds. 11/11 None
F25H2.8.2 ENSEMBL cdna:known chromosome:CEL140:I:10571089:10573035:1 gene:F25H2.8 11/11 None
F25H2.8.1 ENSEMBL cdna:known chromosome:CEL140:I:10571091:10573026:1 gene:F25H2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10572652-10572904(+) 83.67 83.67
Public Domain and Genome References
Gene Title
Gene Symbol ubc-25
Ensembl F25H2.8
Entrez Gene 172941 Entrez gene
SwissProt Q93571 EMBL-EBI
WormBase CE34543 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604012_AT similar to CG2924-PC, isoform C cfa
CANINE_2:CFA.244.1.S1_AT similar to CG2924-PC, isoform C cfa
CANINE_2:CFAAFFX.27795.1.S1_S_AT similar to CG2924-PC, isoform C cfa
DROSOPHILA_2:1637905_S_AT dm
DROSGENOME1:153287_AT dm
CHICKEN:GGA.5694.1.S1_AT similar to MGC68587 protein gga
HU35KSUBC:RC_N25939_AT ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HU35KSUBD:RC_AA476260_AT ubiquitin-conjugating enzyme E2Q (putative) 2 hs
U133_X3P:HS.95262.0.S1_3P_AT ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HG-U133_PLUS_2:224747_AT ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HG-U133B:224747_AT ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HG-U95C:63907_I_AT Ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HG-U95C:63908_R_AT Ubiquitin-conjugating enzyme E2Q (putative) 2 hs
HU35KSUBB:RC_R46086_AT Ubiquitin-conjugating enzyme E2Q (putative) 2 hs
RG-U34B:RC_AI070030_AT similar to RIKEN cDNA 3010021M21 (predicted) rn
RAE230B:1393072_AT similar to RIKEN cDNA 3010021M21 (predicted) rn
RAT230_2:1393072_AT similar to RIKEN cDNA 3010021M21 (predicted) rn
RAE230A:1376314_AT Similar to RIKEN cDNA 3010021M21 (predicted) rn
RAT230_2:1376314_AT Similar to RIKEN cDNA 3010021M21 (predicted) rn
RG-U34A:RC_AI639308_AT Similar to RIKEN cDNA 3010021M21 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02096 Hypothetical protein F25H2.8 [Caenorhabditis elegans] ref|NP_492764.2| UBiquitin Conjugating enzyme family member (ubc-25) [Caenorhabditis elegans] 0.0
blast AAO85552 ubiquitin conjugating enzyme UBC-25 [Caenorhabditis elegans] 0.0
blast CAE58979 Hypothetical protein CBG02252 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:173052_AT
aaacatgtgtaaatcatctgaaatttngagtttcacgcgatggagtgttcacaaacccaa
catgttccaagaaacgaacgcattcccctcatttcgtttttttgtatagtttttagcttc
ctttctcctataccaaaaactgtccagacgtctgaa

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACATGTGTAAATCATCTGAAATT 105 129 113 Antisense
GAGTTTCACGCGATGGAGTGTTCAC 426 397 140 Antisense
GCGATGGAGTGTTCACAAACCCAAC 253 291 149 Antisense
GAGTGTTCACAAACCCAACATGTTC 362 401 155 Antisense
GTTCACAAACCCAACATGTTCCAAG 449 445 159 Antisense
TCACAAACCCAACATGTTCCAAGAA 681 627 161 Antisense
TCCAAGAAACGAACGCATTCCCCTC 109 585 178 Antisense
TGTATAGTTTTTAGCTTCCTTTCTC 195 561 215 Antisense
TTCCTTTCTCCTATACCAAAAACTG 104 695 230 Antisense
CTCCTATACCAAAAACTGTCCAGAC 55 233 237 Antisense
ACCAAAAACTGTCCAGACGTCTGAA 381 83 244 Antisense
Sequence Source Affymetrix Proprietary Database