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[173041_s_at]

Description CEC3444_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C11344 /DEF=C.elegans cDNA clone yk171b10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 95      6grp_total > 77      4grp_total > 40      6grp_highest_sub > Da      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 432
1 2 3 4 range
A 470.5
(-3)
P 		757.4
(3)
P 		710.8
(2)
P 		648.5
(0.7)
P 		287
B 548.4
(-3)
P 		749.5
(0.4)
P 		902.1
(3)
P 		693.7
(-0.5)
P 		354
C 619.4
(-3)
P 		669.7
(-1.8)
P 		871
(3)
P 		808.5
(1.5)
P 		252
avr 546.1
(-3) 		725.5
(0.8) 		828
(3) 		716.9
(0.6) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 914
1 2 3 4 5 6 range
A 466.7
(-3)
P 		548.6
(-2.2)
P 		701.4
(-0.7)
P 		601.1
(-1.7)
P 		701.7
(-0.7)
P 		1077.9
(3)
P 		611
B 577
(-2.5)
P 		507.8
(-3)
P 		753.6
(-1.2)
P 		535.9
(-2.8)
P 		798.1
(-0.9)
P 		1330
(3)
P 		822
C 533.5
(-2.6)
P 		479.5
(-3)
P 		601.8
(-2.2)
P 		666.3
(-1.8)
P 		828.7
(-0.7)
P 		1380.9
(3)
P 		901
avr 525.7
(-2.9) 		512
(-3) 		685.6
(-1.6) 		601.1
(-2.3) 		776.2
(-0.9) 		1262.9
(3) 		751
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 209
1 2 3 4 range
A 171.3
(2)
P 		164.4
(1.6)
P 		185.8
(3)
P 		94.9
(-3)
P 		91
B 174.9
(-0.3)
P 		185.1
(0.7)
P 		209
(3)
P 		147.8
(-3)
P 		61
C 205.1
(-0.5)
P 		192.5
(-1)
P 		303.6
(3)
P 		136.3
(-3)
P 		167
avr 183.8
(0.2) 		180.7
(0.1) 		232.8
(3) 		126.3
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 410
1 2 3 4 5 6 range
A 86
(-3)
P 		195.1
(0.7)
P 		152.8
(-0.7)
P 		152.4
(-0.7)
P 		211.7
(1.3)
P 		262.4
(3)
P 		176
B 200.8
(-2)
P 		169
(-2.5)
P 		183
(-2.3)
P 		141.9
(-3)
P 		324.3
(0.1)
P 		495.9
(3)
P 		354
C 197.7
(-1.8)
P 		146.3
(-2.8)
P 		153.2
(-2.6)
P 		134
(-3)
P 		268.2
(-0.5)
P 		462.3
(3)
P 		328
avr 161.5
(-2.6) 		170.1
(-2.4) 		163
(-2.5) 		142.8
(-3) 		268.1
(-0.2) 		406.9
(3) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173041_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27144
Sequence Type Exemplar sequence
Representative Public ID CEC3444 NCBI
Target Description CEC3444_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C11344 /DEF=C.elegans cDNA clone yk171b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068926(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068926 NCBI Caenorhabditis elegans Caenorhabditis Zeste White 10 (Drosophila) homolog family member (czw-1) (czw-1) mRNA, complete cds. 11/11 A 
SNAP00000004278 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7943738:7946558:-1 11/11 None
GENEFINDER00000004288 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7943738:7946558:-1 11/11 None
F20D12.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:7943609:7946786:-1 gene:F20D12.4 11/11 A 
F20D12.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:7943609:7946575:-1 gene:F20D12.4 11/11 A 
F20D12.4.3 ENSEMBL cdna:known chromosome:CEL140:IV:7943609:7946802:-1 gene:F20D12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7943686-7944270(-) 81.67 81.67
Public Domain and Genome References
Gene Title
Gene Symbol czw-1
Ensembl F20D12.4
Entrez Gene 177587 Entrez gene
SwissProt Q19642 EMBL-EBI
WormBase CE04434 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:267603_AT centromere/kinetochore protein, putative (ZW10) at
CANINE:1585593_AT similar to Centromere/kinetochore protein zw10 homolog cfa
CANINE_2:CFA.9032.1.A1_AT similar to Centromere/kinetochore protein zw10 homolog cfa
CANINE_2:CFAAFFX.21190.1.S1_S_AT similar to Centromere/kinetochore protein zw10 homolog cfa
DROSOPHILA_2:1634604_AT mitotic 15 dm
DROSGENOME1:141713_AT mitotic 15 dm
CHICKEN:GGA.4252.1.S1_AT ZW10, kinetochore associated, homolog (Drosophila) gga
CHICKEN:GGA.4252.1.S2_AT ZW10, kinetochore associated, homolog (Drosophila) gga
HG-U133_PLUS_2:204812_AT ZW10, kinetochore associated, homolog (Drosophila) hs
HG-U133A:204812_AT ZW10, kinetochore associated, homolog (Drosophila) hs
HG-FOCUS:204812_AT ZW10, kinetochore associated, homolog (Drosophila) hs
HG-U133A_2:204812_AT ZW10, kinetochore associated, homolog (Drosophila) hs
U133_X3P:G4759343_3P_AT ZW10, kinetochore associated, homolog (Drosophila) hs
HG-U95AV2:37854_AT ZW10, kinetochore associated, homolog (Drosophila) hs
MOUSE430_2:1424891_A_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOE430A:1424891_A_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOUSE430A_2:1424891_A_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MU11KSUBA:C79507_RC_F_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MU11KSUBA:AF003951_G_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MU11KSUBA:AF003951_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MG-U74AV2:104757_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MG-U74CV2:137018_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOE430B:1431747_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOUSE430_2:1431747_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOE430B:1445206_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
MOUSE430_2:1445206_AT ZW10 homolog (Drosophila), centromere/kinetochore protein mm
RG-U34C:RC_AI230413_AT ZW10 homolog, centromere/kinetochore protein (Drosophila) (predicted) rn
RAE230A:1374938_AT ZW10 homolog, centromere/kinetochore protein (Drosophila) (predicted) rn
RAT230_2:1374938_AT ZW10 homolog, centromere/kinetochore protein (Drosophila) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA81674 Caenorhabditis zeste white 10 (drosophila) homolog protein 1 [Caenorhabditis elegans] ref|NP_501327.1| Caenorhabditis Zeste White 10 (Drosophila) homolog family member (czw-1) [Caenorhabditis elegans] 0.0
blast CAE70918 Hypothetical protein CBG17717 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR009361 EMBL-EBI Centromerekinetochore Zw10 1.0E-126
Pfam IPR009361 EMBL-EBI Centromerekinetochore Zw10 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173041_S_AT
cgagagtatttccgtcttaatgaaattgtgttcgttcttggaaacagaatgcaggatatc
gagcatcgatggttcaacgctaagggtccaatgncagaacatttgagccgatccgaagtt
gttggactgattaaagcattattcgccgactcncaacatcgttctgatcttatcgctcgt
ttata

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAGAGTATTTCCGTCTTAATGAAA 49 249 70 Antisense
AATGAAATTGTGTTCGTTCTTGGAA 39 165 88 Antisense
GTGTTCGTTCTTGGAAACAGAATGC 157 483 97 Antisense
GAATGCAGGATATCGAGCATCGATG 581 331 116 Antisense
ATCGAGCATCGATGGTTCAACGCTA 548 35 127 Antisense
GATGGTTCAACGCTAAGGGTCCAAT 670 405 137 Antisense
CAGAACATTTGAGCCGATCCGAAGT 239 205 164 Antisense
GAGCCGATCCGAAGTTGTTGGACTG 330 385 174 Antisense
TGATTAAAGCATTATTCGCCGACTC 427 563 197 Antisense
AACATCGTTCTGATCTTATCGCTCG 610 137 224 Antisense
GTTCTGATCTTATCGCTCGTTTATA 316 443 230 Antisense
Sequence Source Affymetrix Proprietary Database