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[173036_s_at] protease

Description C57375_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C57375 /DEF=C.elegans cDNA clone yk302h10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 07      6grp_total > 33      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > 03      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18073
1 2 3 4 range
A 19018.4
(3)
P 		17249.7
(2.4)
P 		14383.2
(1.3)
P 		2522.5
(-3)
P 		16496
B 20595.2
(3)
P 		14180.1
(0.8)
P 		15118.2
(1.1)
P 		2931.6
(-3)
P 		17664
C 17143.1
(3)
P 		14746.9
(2)
P 		14368.2
(1.9)
P 		2584.4
(-3)
P 		14559
avr 18918.9
(3) 		15392.2
(1.7) 		14623.2
(1.4) 		2679.5
(-3) 		16239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8629
1 2 3 4 5 6 range
A 15147.3
(0.6)
P 		15206.2
(0.6)
P 		17819.8
(3)
P 		12667.7
(-1.7)
P 		12025.4
(-2.3)
P 		11229.7
(-3)
P 		6590
B 17859
(3)
P 		14138.7
(0.4)
P 		15107.8
(1.1)
P 		12263.4
(-0.9)
P 		11505.8
(-1.4)
P 		9229.8
(-3)
P 		8629
C 14268.6
(1.8)
P 		13182.2
(0.5)
P 		15316.2
(3)
P 		13600
(1)
P 		13658.9
(1.1)
P 		10206.3
(-3)
P 		5110
avr 15758.3
(2.7) 		14175.7
(1) 		16081.3
(3) 		12843.7
(-0.3) 		12396.7
(-0.8) 		10221.9
(-3) 		5859
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6555
1 2 3 4 range
A 6924.6
(3)
P 		3744.4
(0.1)
P 		3760.1
(0.1)
P 		369.2
(-3)
P 		6555
B 6567.7
(3)
P 		3502.2
(-0.1)
P 		3503.2
(-0.1)
P 		624.7
(-3)
P 		5943
C 5674.8
(3)
P 		4239.8
(1.4)
P 		5007.8
(2.2)
P 		435.8
(-3)
P 		5239
avr 6389
(3) 		3828.8
(0.4) 		4090.4
(0.7) 		476.6
(-3) 		5912

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3480
1 2 3 4 5 6 range
A 2792.3
(-2.9)
P 		5408.1
(3)
P 		3880.8
(-0.4)
P 		3212.8
(-1.9)
P 		3628.7
(-1)
P 		2734.1
(-3)
P 		2674
B 6214.1
(3)
P 		4706.2
(-0.1)
P 		3668.6
(-2.1)
P 		3248.2
(-3)
P 		4674.7
(-0.1)
P 		3441.8
(-2.6)
P 		2966
C 5288.8
(3)
P 		4022.3
(0)
P 		3898.2
(-0.3)
P 		2735
(-3)
P 		4419.8
(1)
P 		3416.7
(-1.4)
P 		2554
avr 4765.1
(3) 		4712.2
(2.8) 		3815.9
(-0.4) 		3065.3
(-3) 		4241.1
(1.2) 		3197.5
(-2.5) 		1700
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173036_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26582
Sequence Type Exemplar sequence
Representative Public ID C57375 NCBI
Target Description C57375_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C57375 /DEF=C.elegans cDNA clone yk302h10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072732(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072732 NCBI Caenorhabditis elegans F21F8.7 (protease) mRNA, complete cds. 11/11 A 
SNAP00000008545 ENSEMBL cdna:SNAP chromosome:CEL140:V:8270204:8271525:-1 11/11 None
GENEFINDER00000008561 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8270204:8271525:-1 11/11 None
F21F8.7.1 ENSEMBL cdna:known chromosome:CEL140:V:8270009:8271527:-1 gene:F21F8.7 11/11 None
F21F8.7.2 ENSEMBL cdna:known chromosome:CEL140:V:8270147:8271529:-1 gene:F21F8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8270282-8270515(-) 77.67 77.67
Public Domain and Genome References
Gene Title protease
Gene Symbol asp-6
Ensembl F21F8.7
Entrez Gene 179209 Entrez gene
SwissProt O01530 EMBL-EBI
WormBase CE09542 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47996 Aspartyl protease protein 6 [Caenorhabditis elegans] ref|NP_505133.1| F21F8.7 [Caenorhabditis elegans] 0.0
blast CAE66088 Hypothetical protein CBG11305 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 4.5E-74
Sequence
Target Sequence 
>C. ELEGANS:173036_S_AT
caaggccgctggagccacttatgatgactttaacgaggtctacttcatcgactgcgctgc
ccagccaggaactcttgatattaccattggaaccaacacttattccatccagccagtcaa
ctacatcgttgatgccggaaacggtcaatgccttttcgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGGCCGCTGGAGCCACTTATGAT 327 179 44 Antisense
CCGCTGGAGCCACTTATGATGACTT 408 263 49 Antisense
GGAGCCACTTATGATGACTTTAACG 34 523 54 Antisense
GATGACTTTAACGAGGTCTACTTCA 228 411 66 Antisense
AACGAGGTCTACTTCATCGACTGCG 212 145 75 Antisense
CAGCCAGGAACTCTTGATATTACCA 151 203 105 Antisense
AACTCTTGATATTACCATTGGAACC 242 141 113 Antisense
GGAACCAACACTTATTCCATCCAGC 434 529 132 Antisense
CAACTACATCGTTGATGCCGGAAAC 335 189 161 Antisense
GATGCCGGAAACGGTCAATGCCTTT 251 415 174 Antisense
GGAAACGGTCAATGCCTTTTCGCTG 503 525 180 Antisense
Sequence Source Affymetrix Proprietary Database