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[173022_at] glucosamine-fructose-6-phosphate aminotransferase

Description C52957_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52957 /DEF=C.elegans cDNA clone yk286f8 : 3prime end, single read.
Cluster 6grp_total > 95      4grp_total > 12      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 23.8
(-3)
A 		71.8
(0.2)
A 		33.4
(-2.4)
A 		113.3
(3)
A 		90
B 57
(2.3)
A 		28.6
(-3)
A 		44.6
(-0)
A 		60.9
(3)
A 		32
C 62.3
(3)
A 		23.2
(-2.6)
A 		20.7
(-3)
A 		36.3
(-0.8)
A 		42
avr 47.7
(-0.6) 		41.2
(-1.7) 		32.9
(-3) 		70.2
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 8.7
(-3)
A 		11.1
(-2.9)
A 		25
(-2.4)
A 		20.2
(-2.6)
A 		162.8
(3)
A 		13.3
(-2.8)
A 		154
B 7.5
(-2.8)
A 		3.6
(-3)
A 		8.7
(-2.7)
A 		88.3
(2)
A 		20.8
(-2)
A 		105
(3)
A 		101
C 13.7
(-2.6)
A 		7.1
(-3)
A 		27.3
(-1.9)
A 		52.4
(-0.5)
A 		81
(1)
A 		117.8
(3)
A 		111
avr 10
(-2.8) 		7.3
(-3) 		20.3
(-2) 		53.6
(0.4) 		88.2
(3) 		78.7
(2.3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 8.7
(-3)
A 		15.6
(2.2)
A 		8.7
(-3)
A 		16.6
(3)
A 		8
B 18.2
(3)
A 		7.1
(-3)
A 		10.3
(-1.3)
A 		13
(0.2)
A 		11
C 20.6
(3)
A 		6.7
(-2.8)
A 		7.2
(-2.5)
A 		6.1
(-3)
A 		15
avr 15.8
(3) 		9.8
(-2.1) 		8.7
(-3) 		11.9
(-0.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 1.6
(-3)
A 		4
(-2.7)
A 		5.4
(-2.5)
A 		5.1
(-2.6)
A 		49.1
(3)
A 		3.2
(-2.8)
A 		48
B 2.6
(-2.8)
A 		1.2
(-3)
A 		2.1
(-2.9)
A 		23.4
(0.5)
A 		8.5
(-1.8)
A 		39.1
(3)
A 		38
C 5.1
(-2.5)
A 		2.2
(-3)
A 		7
(-2.2)
A 		10.5
(-1.7)
A 		26.2
(0.9)
A 		39.5
(3)
A 		37
avr 3.1
(-2.9) 		2.5
(-3) 		4.8
(-2.5) 		13
(-0.5) 		27.9
(3) 		27.3
(2.9) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173022_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26473
Sequence Type Exemplar sequence
Representative Public ID C52957 NCBI
Target Description C52957_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52957 /DEF=C.elegans cDNA clone yk286f8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069755
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000028588 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11410484:11412816:1 None
F22B3.4 ENSEMBL cdna:known chromosome:CEL140:IV:11410456:11412955:1 gene:F22B3.4 A 
NM_069755 NCBI Caenorhabditis elegans F22B3.4 (F22B3.4) mRNA, complete cds. A 
GENEFINDER00000028600 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11410484:11412816:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11412680-11412907(+) 57.67 57.67
Public Domain and Genome References
Gene Title glucosamine-fructose-6-phosphate aminotransferase
Ensembl F22B3.4
Entrez Gene 178067 Entrez gene
SwissProt Q19699 EMBL-EBI
WormBase CE03255 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256911_AT glucosamine--fructose-6-phosphate aminotransferase (isomerizing), putative / hexosephosphate aminotransferase, putative / glucosamine-6-phosphate synthase, putative / D-fructose-6-phosphate amidotransferase, putative / GLCN6P synthase, putative at
CANINE:1589341_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
CANINE_2:CFA.12553.1.A1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
CANINE_2:CFAAFFX.1631.1.S1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
DROSOPHILA_2:1635153_AT Glutamine:fructose-6-phosphate aminotransferase 2 dm
DROSGENOME1:152464_AT Glutamine:fructose-6-phosphate aminotransferase 2 dm
CHICKEN:GGAAFFX.8765.1.S1_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
CHICKEN:GGAAFFX.8765.3.S1_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
CHICKEN:GGAAFFX.8765.3.S1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
HG-U133_PLUS_2:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133A:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-FOCUS:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133A_2:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HU35KSUBA:RC_AA037803_AT glutamine-fructose-6-phosphate transaminase 2 hs
U133_X3P:G4826741_3P_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U95AV2:39640_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133_PLUS_2:237352_AT Glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133B:237352_AT Glutamine-fructose-6-phosphate transaminase 2 hs
HG-U95D:71881_R_AT Glutamine-fructose-6-phosphate transaminase 2 hs
U133_X3P:HS.220741.0.A1_3P_AT Glutamine-fructose-6-phosphate transaminase 2 hs
MOE430A:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MOUSE430A_2:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MOUSE430_2:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MG-U74AV2:103737_AT glutamine fructose-6-phosphate transaminase 2 mm
YEAST_2:1778809_AT Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
16051 carbohydrate biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4360 glutamine-fructose-6-phosphate transaminase (isomerizing) activity inferred from electronic annotation QuickGO AmiGO
5529 sugar binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92735 Hypothetical protein F22B3.4 [Caenorhabditis elegans] ref|NP_502156.1| F22B3.4 [Caenorhabditis elegans] 0.0
blast CAE59466 Hypothetical protein CBG02850 [Caenorhabditis briggsae] 0.0
blast CAC42276 Hypothetical protein F07A11.2b [Caenorhabditis elegans] ref|NP_496479.1| F07A11.2b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000583 EMBL-EBI Glutamine amidotransferase, class-II 1.6E-55
Pfam IPR001347 EMBL-EBI Sugar isomerase (SIS) 7.0E-43
Pfam IPR001347 EMBL-EBI Sugar isomerase (SIS) 1.3E-29
Sequence
Target Sequence 
>C. ELEGANS:173022_AT
gggcgttttttattggtgagaatcgcagcaacttgtcgcgccaatcgagccatgtcgtcg
tcaacgaccccccattcaagaacagcaagcagcattgagaactttggaatccagtcccta
tgcagcttctctcataccacattgctgagctcaatggagcaaacgtggaccgaccacgca
atttggcaaaatctgtcactgttgagtaagccaatccgtcaaccacatccttgctcaatg
actactcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGCGTTTTTTATTGGTGAGAATCG 103 341 19 Antisense
AGAATCGCAGCAACTTGTCGCGCCA 620 75 37 Antisense
TCGAGCCATGTCGTCGTCAACGACC 201 601 63 Antisense
ACCCCCCATTCAAGAACAGCAAGCA 164 91 85 Antisense
ACAGCAAGCAGCATTGAGAACTTTG 63 109 100 Antisense
GAGAACTTTGGAATCCAGTCCCTAT 291 391 115 Antisense
CTTTGGAATCCAGTCCCTATGCAGC 55 223 120 Antisense
GTCACTGTTGAGTAAGCCAATCCGT 246 465 213 Antisense
TTGAGTAAGCCAATCCGTCAACCAC 115 705 220 Antisense
GTCAACCACATCCTTGCTCAATGAC 375 463 236 Antisense
ACATCCTTGCTCAATGACTACTCAC 356 107 243 Antisense
Sequence Source Affymetrix Proprietary Database