[Home] | [Total List]

[173014_at] neprilysin

Description CEK004E1R_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=D27826 /DEF=C.elegans cDNA clone yk4e1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 94      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 54      6grp_total > 85      4grp_total > 16      6grp_highest_sub > 04_05_06      6grp_cgc > 77      6grp_high1_sub > In      6grp_cgc_sub > 04_05_06      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2554
1 2 3 4 range
A 772.5
(-3)
P 		847.8
(-2.6)
P 		1584.3
(0.9)
P 		2032.1
(3)
P 		1260
B 649.2
(-3)
P 		1235.6
(-1.6)
P 		1354.2
(-1.3)
P 		3203.5
(3)
P 		2554
C 795.3
(-3)
P 		1177.3
(-1.7)
P 		1670.7
(-0)
P 		2572.8
(3)
P 		1778
avr 739
(-3) 		1086.9
(-1.9) 		1536.4
(-0.4) 		2602.8
(3) 		1864

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1599
1 2 3 4 5 6 range
A 533.7
(-3)
P 		1427.8
(2)
P 		985.5
(-0.5)
P 		1552.1
(2.6)
P 		1615.5
(3)
P 		1373.6
(1.7)
P 		1082
B 642.1
(-3)
P 		1255.3
(0.1)
P 		1035.1
(-1)
P 		1796.7
(2.8)
P 		1283.1
(0.2)
P 		1827.3
(3)
P 		1185
C 549.1
(-3)
P 		1422.8
(0.3)
P 		1237.3
(-0.4)
P 		2132.7
(3)
P 		1253.5
(-0.3)
P 		1751.5
(1.6)
P 		1584
avr 575
(-3) 		1368.6
(0.8) 		1086
(-0.6) 		1827.2
(3) 		1384
(0.9) 		1650.8
(2.2) 		1252
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 499
1 2 3 4 range
A 281.3
(-0.5)
P 		184
(-3)
P 		414.2
(3)
P 		297.5
(-0)
P 		230
B 207
(-3)
P 		305.2
(-1.8)
P 		313.8
(-1.7)
P 		682.7
(3)
P 		476
C 263.3
(-3)
P 		338.5
(-1.6)
P 		582.3
(3)
P 		433.8
(0.2)
P 		319
avr 250.5
(-3) 		275.9
(-2.3) 		436.8
(2.1) 		471.3
(3) 		221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 583
1 2 3 4 5 6 range
A 98.4
(-3)
P 		507.8
(3)
P 		214.6
(-1.3)
P 		393.6
(1.3)
P 		487.5
(2.7)
P 		334.4
(0.5)
P 		409
B 223.4
(-3)
P 		417.8
(-0.5)
P 		251.4
(-2.6)
P 		475.9
(0.3)
P 		521.3
(0.9)
P 		681.4
(3)
P 		458
C 203.5
(-3)
P 		434.1
(0.6)
P 		314.9
(-1.3)
P 		428.9
(0.5)
P 		405.6
(0.2)
P 		586.3
(3)
P 		383
avr 175.1
(-3) 		453.2
(1.6) 		260.3
(-1.6) 		432.8
(1.3) 		471.5
(2) 		534
(3) 		359
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173014_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27325
Sequence Type Exemplar sequence
Representative Public ID CEK004E1R NCBI
Target Description CEK004E1R_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=D27826 /DEF=C.elegans cDNA clone yk4e1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064089(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064089 NCBI Caenorhabditis elegans ZK20.6 (neprilysin) mRNA, complete cds. 9/11 None
ZK20.6 ENSEMBL cdna:known chromosome:CEL140:II:11654129:11657354:-1 gene:ZK20.6 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11654139-11654846(-) 96.72 96.72
Public Domain and Genome References
Gene Title neprilysin
Gene Symbol nep-1
Ensembl ZK20.6
Entrez Gene 174787 Entrez gene
WormBase CE39118 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.19432.1.S1_AT similar to endothelin converting enzyme 2 isoform 2b-2 cfa
CANINE_2:CFAAFFX.19491.1.S1_S_AT similar to endothelin converting enzyme 2 isoform 2b-2 cfa
HG-U133_PLUS_2:206728_AT endothelin converting enzyme 2 hs
HG-U133A_2:206728_AT endothelin converting enzyme 2 hs
HG-U133A:206728_AT endothelin converting enzyme 2 hs
U133_X3P:G7662199_3P_AT endothelin converting enzyme 2 hs
U133_X3P:HS2.129801.4.S1_3P_S_AT endothelin converting enzyme 2 hs
HG-U133_PLUS_2:1555306_A_AT endothelin converting enzyme 2 hs
HG-U95AV2:35536_AT endothelin converting enzyme 2 hs
HG-U95C:49794_S_AT Endothelin converting enzyme 2 hs
MG-U74CV2:138204_AT endothelin converting enzyme 2 mm
MG-U74CV2:168619_R_AT endothelin converting enzyme 2 mm
MOE430A:1424561_AT endothelin converting enzyme 2 mm
MOUSE430A_2:1424561_AT endothelin converting enzyme 2 mm
MOUSE430_2:1424561_AT endothelin converting enzyme 2 mm
RAE230B:1386749_AT endothelin converting enzyme 2 rn
RAT230_2:1386749_AT endothelin converting enzyme 2 rn
Protein Similarities
Method ID Description E-Value
blast NP_496490 ZK20.6 [Caenorhabditis elegans] 0.0
blast CAA93770 Hypothetical protein ZK20.6 [Caenorhabditis elegans] emb|CAA93782.2| Hypothetical protein ZK20.6 [Caenorhabditis elegans] 0.0
blast CAE59457 Hypothetical protein CBG02837 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 3.4E-82
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.4E-94
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496490.1 1 17-39
ZK20.6 1 17-39
Sequence
Target Sequence 
>C. ELEGANS:173014_AT
tcctcaactcaccagaaacctgcaagagtgaacacgggtctaaccaaatcagccagaatt
tgccgaggctttcaaatgtccagctggatctccgatgaatccaacgaaacgctgtgttgt
gtggtaatcagaaaatacgggaaaggagtcaaccaagttggcacgctttatctatctgtc
tctcagaatctactaaatctttcgtcctttttttttactcatctagctgtatatattgtt
gaaaacaatagacactcgaattcactgtattttgtccctctatgtattttttctcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCAACTCACCAGAAACCTGCAA 120 593 47 Antisense
CACGGGTCTAACCAAATCAGCCAGA 246 199 79 Antisense
GGAGTCAACCAAGTTGGCACGCTTT 601 517 191 Antisense
AAGTTGGCACGCTTTATCTATCTGT 303 155 201 Antisense
ATCTATCTGTCTCTCAGAATCTACT 130 33 216 Antisense
GAATCTACTAAATCTTTCGTCCTTT 637 327 232 Antisense
CCTTTTTTTTTACTCATCTAGCTGT 390 269 252 Antisense
ACTCATCTAGCTGTATATATTGTTG 175 101 263 Antisense
GACACTCGAATTCACTGTATTTTGT 563 365 297 Antisense
GTATTTTGTCCCTCTATGTATTTTT 482 451 313 Antisense
GTCCCTCTATGTATTTTTTCTCGAA 626 475 320 Antisense
Sequence Source Affymetrix Proprietary Database