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[173010_at]

Description C52970_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52970 /DEF=C.elegans cDNA clone yk286h11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 11      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 51      6grp_total > 13      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 625
1 2 3 4 range
A 833.4
(3)
P 		208.1
(-3)
P 		357.5
(-1.6)
P 		317.5
(-2)
P 		625
B 634.1
(3)
P 		349.2
(-1.6)
P 		261.9
(-3)
P 		321.5
(-2)
P 		372
C 725.9
(3)
P 		235.9
(-3)
P 		267.1
(-2.6)
P 		457.1
(-0.3)
P 		490
avr 731.1
(3) 		264.4
(-3) 		295.5
(-2.6) 		365.4
(-1.7) 		467

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 409
1 2 3 4 5 6 range
A 492.6
(0.6)
P 		637.5
(3)
P 		480.8
(0.4)
P 		487.1
(0.5)
P 		392.3
(-1)
P 		273.6
(-3)
P 		364
B 668
(3)
P 		582.4
(1.6)
P 		472.3
(-0.2)
P 		584.8
(1.6)
P 		351.5
(-2.2)
P 		299.8
(-3)
P 		368
C 654.4
(3)
P 		653.8
(3)
P 		630
(2.6)
P 		604.2
(2.2)
P 		259.4
(-3)
P 		320.6
(-2.1)
P 		395
avr 605
(2.6) 		624.6
(3) 		527.7
(1.2) 		558.7
(1.8) 		334.4
(-2.3) 		298
(-3) 		327
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 258
1 2 3 4 range
A 303.4
(3)
P 		45.2
(-3)
P 		93.5
(-1.9)
P 		46.5
(-3)
P 		258
B 202.2
(3)
P 		86.2
(-1.9)
P 		60.7
(-3)
P 		68.5
(-2.7)
P 		142
C 240.3
(3)
P 		67.8
(-3)
P 		93.1
(-2.1)
P 		77.1
(-2.7)
P 		173
avr 248.6
(3) 		66.4
(-2.9) 		82.4
(-2.4) 		64
(-3) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 90.8
(-2.1)
P 		226.7
(3)
P 		104.7
(-1.6)
P 		123.5
(-0.9)
P 		118.4
(-1.1)
P 		66.6
(-3)
P 		160
B 232.4
(3)
P 		193.9
(1.1)
P 		114.7
(-2.9)
P 		154.9
(-0.9)
P 		142.8
(-1.5)
P 		111.8
(-3)
P 		121
C 242.6
(3)
P 		199.5
(1.4)
P 		160.3
(-0.1)
P 		121.5
(-1.6)
P 		83.9
(-3)
P 		107.3
(-2.1)
P 		159
avr 188.6
(2) 		206.7
(3) 		126.6
(-1.3) 		133.3
(-0.9) 		115
(-1.9) 		95.2
(-3) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173010_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26476
Sequence Type Exemplar sequence
Representative Public ID C52970 NCBI
Target Description C52970_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52970 /DEF=C.elegans cDNA clone yk286h11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028188(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028188 NCBI Caenorhabditis elegans T05A12.4a (helicase) mRNA, complete cds. 9/11 None
T05A12.4a ENSEMBL cdna:known chromosome:CEL140:IV:6888965:6897761:1 gene:T05A12.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177453 2 1 NM_001028188 173010_at (9), 188591_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6897482-6897684(+) 67.33 67.33
Public Domain and Genome References
Ensembl T05A12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV28333 Hypothetical protein T05A12.4a [Caenorhabditis elegans] ref|NP_001023359.1| T05A12.4a [Caenorhabditis elegans] 0.0
blast AAV28334 Hypothetical protein T05A12.4b [Caenorhabditis elegans] ref|NP_001023360.1| T05A12.4b [Caenorhabditis elegans] 0.0
blast T32703 hypothetical protein T22D1.7 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 4.4E-24
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-10
Pfam IPR001841 EMBL-EBI Zn-finger, RING 7.7E-6
Sequence
Target Sequence 
>C. ELEGANS:173010_AT
ggatgcaggattttttccgcaacaacattgttagnagagccattccataaatccacggtt
ttttattcttgttaacatgtttattgttcccccgacatttccacatttttttgctttaac
taattaattttttgacacaaaccaaagtttcaccctgttacctcgtttcaattgttgcca
ctaaatcgctc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGCAGGATTTTTTCCGCAACAA 587 513 92 Antisense
GCAGGATTTTTTCCGCAACAACATT 209 309 96 Antisense
AGCCATTCCATAAATCCACGGTTTT 353 83 129 Antisense
GCCATTCCATAAATCCACGGTTTTT 85 279 130 Antisense
TCCACATTTTTTTGCTTTAACTAAT 552 587 191 Antisense
GACACAAACCAAAGTTTCACCCTGT 24 367 225 Antisense
CCCTGTTACCTCGTTTCAATTGTTG 501 257 244 Antisense
CTGTTACCTCGTTTCAATTGTTGCC 707 235 246 Antisense
GTTACCTCGTTTCAATTGTTGCCAC 273 445 248 Antisense
ACCTCGTTTCAATTGTTGCCACTAA 119 89 251 Antisense
TTCAATTGTTGCCACTAAATCGCTC 307 683 258 Antisense
Sequence Source Affymetrix Proprietary Database