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[173006_s_at]

Description C34664_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C34664 /DEF=C.elegans cDNA clone yk414b7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 26      4grp_high_8_8 > 57      6grp_highest_nonorm > 30      6grp_total > 27      4grp_total > 61      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 785
1 2 3 4 range
A 618.6
(0.7)
P 		420.2
(-1.5)
P 		824.7
(3)
P 		283.4
(-3)
P 		541
B 977.2
(3)
P 		726.3
(0.2)
P 		795.3
(1)
P 		432.1
(-3)
P 		545
C 1028.1
(2.7)
P 		598.1
(-0.8)
P 		1068
(3)
P 		326.4
(-3)
P 		742
avr 874.6
(2.8) 		581.5
(-0.4) 		896
(3) 		347.3
(-3) 		549

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1103
1 2 3 4 5 6 range
A 918.7
(1.4)
P 		1077.3
(3)
P 		481.2
(-3)
P 		683
(-1)
P 		803.7
(0.2)
P 		683.4
(-1)
P 		596
B 1393.2
(3)
P 		908.8
(-1.7)
P 		777.3
(-3)
P 		788.7
(-2.9)
P 		793.5
(-2.8)
P 		871.6
(-2.1)
P 		616
C 1584.6
(3)
P 		955
(-1.3)
P 		703.8
(-3)
P 		720.7
(-2.9)
P 		704.8
(-3)
P 		955.5
(-1.3)
P 		881
avr 1298.8
(3) 		980.4
(0) 		654.1
(-3) 		730.8
(-2.3) 		767.3
(-1.9) 		836.8
(-1.3) 		645
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 331
1 2 3 4 range
A 225.2
(3)
P 		91.2
(-1.4)
P 		215.6
(2.7)
P 		41.5
(-3)
P 		184
B 311.6
(3)
P 		179.4
(-0.6)
P 		184.3
(-0.5)
P 		92.1
(-3)
P 		220
C 340.3
(2.4)
P 		172
(-0.8)
P 		372.2
(3)
P 		55
(-3)
P 		317
avr 292.4
(3) 		147.5
(-0.8) 		257.4
(2.1) 		62.9
(-3) 		230

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 483
1 2 3 4 5 6 range
A 169.4
(-1.6)
P 		383.2
(3)
P 		104.8
(-3)
P 		173.2
(-1.5)
P 		242.5
(-0)
P 		166.4
(-1.7)
P 		278
B 484.8
(3)
P 		302.5
(-0.7)
P 		188.8
(-3)
P 		208.9
(-2.6)
P 		322.4
(-0.3)
P 		325
(-0.2)
P 		296
C 587.3
(3)
P 		291.4
(-1)
P 		179.1
(-2.5)
P 		144.9
(-3)
P 		228.1
(-1.9)
P 		319.8
(-0.6)
P 		442
avr 413.8
(3) 		325.7
(0.9) 		157.6
(-3) 		175.7
(-2.6) 		264.3
(-0.5) 		270.4
(-0.4) 		256
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173006_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26348
Sequence Type Exemplar sequence
Representative Public ID C34664 NCBI
Target Description C34664_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C34664 /DEF=C.elegans cDNA clone yk414b7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068768(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068768 NCBI Caenorhabditis elegans F42C5.9 (F42C5.9) mRNA, complete cds. 11/11 None
F42C5.9.1 ENSEMBL cdna:known chromosome:CEL140:IV:7314358:7317087:-1 gene:F42C5.9 11/11 None
F42C5.9.2 ENSEMBL cdna:known chromosome:CEL140:IV:7314365:7317346:-1 gene:F42C5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7314451-7314723(-) 88.33 88.33
Public Domain and Genome References
Gene Title
Ensembl F42C5.9
Entrez Gene 177504 Entrez gene
SwissProt Q20329 EMBL-EBI
WormBase CE04562 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
15629 actin cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5200 structural constituent of cytoskeleton inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81487 Hypothetical protein F42C5.9 [Caenorhabditis elegans] ref|NP_501169.1| F42C5.9 [Caenorhabditis elegans] 0.0
blast CAE61856 Hypothetical protein CBG05834 [Caenorhabditis briggsae] 0.0
blast XP_785771 PREDICTED: similar to actin 87E (61.9 kD) (4I80) [Strongylocentrotus purpuratus] 4.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 5.0E-41
Sequence
Target Sequence 
>C. ELEGANS:173006_S_AT
gaagcttcgacctgaaaaataatgatttcgttaatttcttatgatttcactgaccaacct
gaaaattccgctttagaggctcattttcctgctttttcgagagttttctttagaccttcg
tgtactattcgcttttgtacaattattttccttttgtttatatagattttatgatgtccc
gagagctnttttagnacatatttttgtgatactgttgtcgtaat

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTTCGACCTGAAAAATAATGA 336 341 55 Antisense
GATTTCGTTAATTTCTTATGATTTC 173 429 78 Antisense
TCTTATGATTTCACTGACCAACCTG 680 613 91 Antisense
CACTGACCAACCTGAAAATTCCGCT 225 199 102 Antisense
GAAAATTCCGCTTTAGAGGCTCATT 14 353 115 Antisense
TTAGAGGCTCATTTTCCTGCTTTTT 571 681 127 Antisense
TTTCCTGCTTTTTCGAGAGTTTTCT 619 669 139 Antisense
GAGAGTTTTCTTTAGACCTTCGTGT 563 387 153 Antisense
AGACCTTCGTGTACTATTCGCTTTT 379 69 166 Antisense
TCGCTTTTGTACAATTATTTTCCTT 183 601 183 Antisense
ATTTTTGTGATACTGTTGTCGTAAT 537 15 254 Antisense
Sequence Source Affymetrix Proprietary Database