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[172996_s_at]

Description C32344_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C32344 /DEF=C.elegans cDNA clone yk329c9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 72      6grp_total > 39      4grp_total > 62      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 242
1 2 3 4 range
A 495.6
(3)
P 		379.8
(0.1)
P 		454.7
(2)
P 		254.1
(-3)
P 		242
B 484.2
(3)
P 		341.3
(-1.8)
P 		364
(-1)
P 		306.1
(-3)
P 		178
C 452.3
(3)
P 		353.8
(-0.4)
P 		429.7
(2.2)
P 		277.7
(-3)
P 		175
avr 477.4
(3) 		358.3
(-0.6) 		416.1
(1.1) 		279.3
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 433.6
(0.8)
P 		383.3
(-0.6)
P 		354.1
(-1.4)
P 		298.5
(-3)
P 		509.2
(3)
P 		413.5
(0.3)
P 		211
B 520.8
(2.1)
P 		491.5
(1.1)
P 		374.3
(-3)
P 		415.6
(-1.6)
P 		421.4
(-1.4)
P 		547
(3)
P 		173
C 449.4
(1.5)
P 		504.7
(3)
P 		294.1
(-2.9)
P 		290.3
(-3)
P 		355.3
(-1.2)
P 		420.2
(0.6)
P 		214
avr 467.9
(3) 		459.8
(2.6) 		340.8
(-2.7) 		334.8
(-3) 		428.6
(1.2) 		460.2
(2.7) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 180.4
(3)
P 		82.5
(-1.1)
P 		118.9
(0.4)
P 		37.2
(-3)
P 		143
B 154.4
(3)
P 		84.3
(-1.7)
P 		84.3
(-1.7)
P 		65.2
(-3)
P 		89
C 149.7
(3)
P 		101.7
(0.2)
P 		149.8
(3)
P 		46.8
(-3)
P 		103
avr 161.5
(3) 		89.5
(-0.9) 		117.7
(0.6) 		49.7
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 79.9
(-2.7)
P 		136.3
(1.7)
P 		77.1
(-2.9)
P 		75.7
(-3)
P 		153.6
(3)
P 		100.7
(-1.1)
P 		78
B 181.2
(1.8)
P 		163.6
(0.9)
P 		90.9
(-3)
P 		110.1
(-2)
P 		171.2
(1.3)
P 		204
(3)
P 		113
C 166.6
(3)
P 		154
(2.3)
P 		74.9
(-2.1)
P 		58.4
(-3)
P 		115
(0.1)
P 		140.7
(1.6)
P 		108
avr 142.6
(2.3) 		151.3
(3) 		81
(-3) 		81.4
(-3) 		146.6
(2.6) 		148.5
(2.8) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172996_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26315
Sequence Type Exemplar sequence
Representative Public ID C32344 NCBI
Target Description C32344_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C32344 /DEF=C.elegans cDNA clone yk329c9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075113(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075113 NCBI Caenorhabditis elegans Y59A8B.2 (Y59A8B.2) mRNA, complete cds. 11/11 A 
Y59A8B.2 ENSEMBL cdna:known chromosome:CEL140:V:17932237:17936631:1 gene:Y59A8B.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028688 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17936080-17936738(+) 89.0 89.0
Public Domain and Genome References
Gene Title
Ensembl Y59A8B.2
Entrez Gene 3564997 Entrez gene
SwissProt Q9GRZ4 EMBL-EBI
WormBase CE26210 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC14405 Hypothetical protein Y59A8B.2 [Caenorhabditis elegans] ref|NP_507514.1| Y59A8B.2 [Caenorhabditis elegans] 0.0
blast CAE61651 Hypothetical protein CBG05585 [Caenorhabditis briggsae] 1.0E-170
blast AAH43910 Usp8-prov protein [Xenopus laevis] 4.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.4E-95
Sequence
Target Sequence 
>C. ELEGANS:172996_S_AT
gtcgcctcaattcgggacactatacggctgtggcttcacatcttcgctctgacaaatggc
ttcgtttcgatgattctgtcgtgacaccgtgtgaaaatttcaaagttgatccatcgctcg
cctacattctcttctacaaacggtgctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGCCTCAATTCGGGACACTATAC 147 475 56 Antisense
GGACACTATACGGCTGTGGCTTCAC 160 525 70 Antisense
TATACGGCTGTGGCTTCACATCTTC 637 659 76 Antisense
TCACATCTTCGCTCTGACAAATGGC 348 627 91 Antisense
CGCTCTGACAAATGGCTTCGTTTCG 163 255 100 Antisense
GGCTTCGTTTCGATGATTCTGTCGT 659 537 113 Antisense
GTTTCGATGATTCTGTCGTGACACC 344 449 119 Antisense
GATTCTGTCGTGACACCGTGTGAAA 55 433 127 Antisense
CGTGACACCGTGTGAAAATTTCAAA 168 245 135 Antisense
CCTACATTCTCTTCTACAAACGGTG 99 269 176 Antisense
CATTCTCTTCTACAAACGGTGCTAA 662 213 180 Antisense
Sequence Source Affymetrix Proprietary Database