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[172982_s_at]

Description C55226_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C55226 /DEF=C.elegans cDNA clone yk395b1 : 3prime end, single read.
Cluster 6grp_total > 08      4grp_total > 37      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 80.1
(3)
A 		33.6
(-0.6)
A 		3.5
(-3)
A 		45.4
(0.3)
A 		77
B 65
(3)
A 		46.2
(1)
A 		8
(-3)
A 		64.5
(2.9)
A 		57
C 52.6
(0.3)
A 		75.2
(3)
A 		31.5
(-2.3)
A 		25.3
(-3)
A 		50
avr 65.9
(3) 		51.7
(1.3) 		14.3
(-3) 		45.1
(0.6) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 8.7
(-1.8)
A 		29.6
(3)
A 		3.4
(-3)
A 		11.1
(-1.2)
A 		9.5
(-1.6)
A 		4.8
(-2.7)
A 		26
B 41.6
(3)
A 		18.1
(-0.9)
A 		5.6
(-3)
A 		40.9
(2.9)
A 		5.8
(-3)
A 		22.8
(-0.1)
A 		36
C 27.1
(1.5)
A 		4.7
(-2.5)
A 		35.7
(3)
A 		15.6
(-0.6)
A 		2.1
(-3)
A 		4
(-2.7)
A 		34
avr 25.8
(3) 		17.5
(0.5) 		14.9
(-0.3) 		22.5
(2) 		5.8
(-3) 		10.5
(-1.6) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 29.2
(3)
A 		7.3
(-1.6)
A 		0.9
(-3)
A 		6.6
(-1.8)
A 		28
B 20.7
(3)
A 		11.4
(0)
A 		1.9
(-3)
A 		13.7
(0.8)
A 		19
C 17.4
(1.5)
A 		21.6
(3)
A 		11
(-0.7)
A 		4.3
(-3)
A 		17
avr 22.4
(3) 		13.4
(-0) 		4.6
(-3) 		8.2
(-1.8) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 1.6
(-2.5)
A 		10.5
(3)
A 		0.8
(-3)
A 		2.8
(-1.8)
A 		2.9
(-1.7)
A 		1.2
(-2.8)
A 		10
B 14.5
(3)
A 		6
(-0.9)
A 		1.4
(-3)
A 		10.8
(1.3)
A 		2.4
(-2.5)
A 		8.5
(0.3)
A 		13
C 10
(3)
A 		1.4
(-2.5)
A 		9.1
(2.4)
A 		3.1
(-1.5)
A 		0.7
(-3)
A 		1.3
(-2.6)
A 		9
avr 8.7
(3) 		6
(0.6) 		3.8
(-1.4) 		5.6
(0.2) 		2
(-3) 		3.7
(-1.5) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172982_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26537
Sequence Type Exemplar sequence
Representative Public ID C55226 NCBI
Target Description C55226_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C55226 /DEF=C.elegans cDNA clone yk395b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067883(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067883 NCBI Caenorhabditis elegans Y67D8C.5 (Y67D8C.5) mRNA, complete cds. 11/11 A 
SNAP00000001608 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3033190:3049968:-1 11/11 None
GENEFINDER00000001628 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3033190:3049968:-1 11/11 None
Y67D8C.5 ENSEMBL cdna:known chromosome:CEL140:IV:3032726:3049968:-1 gene:Y67D8C.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3042137-3042406(-) 89.67 89.67
Public Domain and Genome References
Gene Title
Ensembl Y67D8C.5
Entrez Gene 177084 Entrez gene
SwissProt Q9GUP2 EMBL-EBI
WormBase CE31665 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG23375 Hypothetical protein Y67D8C.5 [Caenorhabditis elegans] ref|NP_500284.2| Y67D8C.5 [Caenorhabditis elegans] 0.0
blast CAE59883 Hypothetical protein CBG03363 [Caenorhabditis briggsae] 0.0
blast CAI42656 upstream regulatory element binding protein 1 (UREB1) [Homo sapiens] emb|CAI39581.1| upstream regulatory element binding protein 1 (UREB1) [Homo sapiens] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR010309 EMBL-EBI Protein of unknown function DUF908 1.0E-126
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 6.2E-9
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR010314 EMBL-EBI Protein of unknown function DUF913 1.0E-126
Pfam IPR010309 EMBL-EBI Protein of unknown function DUF908 1.0E-126
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 6.2E-9
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR010314 EMBL-EBI Protein of unknown function DUF913 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:172982_S_AT
cgatccgtggatatattgctgttccaactcaacctccggcttcgatgtcagaatatgcag
ctggtaaatcgaaggatttcgccgatactctcaacttcatggctccactttcaacgagaa
atcctctggttttcactgaagcgatgaataagttggcaagaatgaacggatccttgattt
taccaatgatcaaggagaagaagccaacattgtccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATCCGTGGATATATTGCTGTTCC 279 249 13 Antisense
ACCTCCGGCTTCGATGTCAGAATAT 108 89 44 Antisense
GAATATGCAGCTGGTAAATCGAAGG 708 325 63 Antisense
GAAGGATTTCGCCGATACTCTCAAC 322 335 83 Antisense
GCCGATACTCTCAACTTCATGGCTC 334 283 93 Antisense
GGCTCCACTTTCAACGAGAAATCCT 692 539 113 Antisense
ACGAGAAATCCTCTGGTTTTCACTG 495 93 126 Antisense
AATCCTCTGGTTTTCACTGAAGCGA 575 167 132 Antisense
AGTTGGCAAGAATGAACGGATCCTT 264 63 163 Antisense
TGAACGGATCCTTGATTTTACCAAT 607 571 175 Antisense
AGGAGAAGAAGCCAACATTGTCCGT 149 57 205 Antisense
Sequence Source Affymetrix Proprietary Database