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[172979_at]

Description C52077_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52077 /DEF=C.elegans cDNA clone yk226c7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 86      6grp_total > 69      4grp_total > 55      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 641
1 2 3 4 range
A 1283
(3)
P 		641.7
(-3)
P 		928.1
(-0.3)
P 		669.7
(-2.7)
P 		641
B 839.8
(-2.2)
P 		852.4
(-1.8)
P 		818.2
(-3)
P 		990.5
(3)
P 		172
C 1122.4
(3)
P 		925.4
(-2.1)
P 		928.1
(-2)
P 		890.3
(-3)
P 		232
avr 1081.7
(3) 		806.5
(-3) 		891.5
(-1.1) 		850.2
(-2) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 737
1 2 3 4 5 6 range
A 799.4
(-1.8)
P 		944.8
(-0.5)
P 		662.1
(-3)
P 		813.5
(-1.7)
P 		1000
(0)
P 		1337.5
(3)
P 		675
B 938.2
(0.5)
P 		1060.8
(1.8)
P 		600.5
(-3)
P 		992.4
(1.1)
P 		806.4
(-0.9)
P 		1175.2
(3)
P 		575
C 993
(1.9)
P 		1041.8
(3)
P 		1003.8
(2.1)
P 		777.4
(-3)
P 		1007.6
(2.2)
P 		917.9
(0.2)
P 		264
avr 910.2
(-0.6) 		1015.8
(1) 		755.5
(-3) 		861.1
(-1.4) 		938
(-0.2) 		1143.5
(3) 		388
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 369
1 2 3 4 range
A 467.1
(3)
P 		139.3
(-2.3)
P 		242.6
(-0.6)
P 		98
(-3)
P 		369
B 267.8
(3)
P 		210.5
(-1.4)
P 		189.6
(-3)
P 		211.1
(-1.4)
P 		78
C 371.6
(3)
P 		266.1
(0.1)
P 		323.5
(1.7)
P 		150.1
(-3)
P 		222
avr 368.8
(3) 		205.3
(-1.5) 		251.9
(-0.3) 		153.1
(-3) 		216

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 147.4
(-2.9)
P 		336
(3)
P 		144.2
(-3)
P 		206.3
(-1.1)
P 		301.8
(1.9)
P 		325.6
(2.7)
P 		192
B 326.4
(0.7)
P 		353.1
(1.3)
P 		145.8
(-3)
P 		262.8
(-0.6)
P 		327.6
(0.7)
P 		438.2
(3)
P 		292
C 368.1
(3)
P 		317.9
(1.6)
P 		255.5
(-0.2)
P 		156.3
(-3)
P 		326.1
(1.8)
P 		307.3
(1.3)
P 		212
avr 280.6
(0.4) 		335.7
(2.3) 		181.8
(-3) 		208.5
(-2.1) 		318.5
(1.7) 		357
(3) 		175
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172979_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26449
Sequence Type Exemplar sequence
Representative Public ID C52077 NCBI
Target Description C52077_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C52077 /DEF=C.elegans cDNA clone yk226c7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059441(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059441 NCBI Caenorhabditis elegans defective in Germ Line Development family member (gld-2) (gld-2) mRNA, complete cds. 11/11 A 
ZC308.1a ENSEMBL cdna:known chromosome:CEL140:I:6378100:6394278:1 gene:ZC308.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172338 4 1 NM_059440 182181_at (11)
NM_059441 172979_at (11), 174893_at (11), 182981_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6393884-6394163(+) 80.0 92.0
Public Domain and Genome References
Ensembl ZC308.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70342 Defective in germ line development protein 2, isoform a [Caenorhabditis elegans] ref|NP_491842.2| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] gb|AAM94369.1| regulatory cytoplasmic polyA polymerase [Caenorhabditis elegans] 0.0
blast AAO21479 Defective in germ line development protein 2, isoform d [Caenorhabditis elegans] ref|NP_001021845.1| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] 0.0
blast AAO21478 Defective in germ line development protein 2, isoform c [Caenorhabditis elegans] ref|NP_491841.2| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 9.8E-11
Sequence
Target Sequence 
>C. ELEGANS:172979_AT
tatatcattttttttctcgtcacatgggtgttcatttttctattgctcttttttcggttc
atcagaactttccacaattcctgttctgtaattttctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATATCATTTTTTTTCTCGTCACAT 416 661 184 Antisense
ATTTTTTTTCTCGTCACATGGGTGT 83 15 190 Antisense
TTTTCTCGTCACATGGGTGTTCATT 589 675 195 Antisense
GTCACATGGGTGTTCATTTTTCTAT 564 463 202 Antisense
TCTATTGCTCTTTTTTCGGTTCATC 109 615 222 Antisense
TATTGCTCTTTTTTCGGTTCATCAG 481 665 224 Antisense
GCTCTTTTTTCGGTTCATCAGAACT 685 299 228 Antisense
GGTTCATCAGAACTTTCCACAATTC 359 507 239 Antisense
ATCAGAACTTTCCACAATTCCTGTT 365 29 244 Antisense
ACTTTCCACAATTCCTGTTCTGTAA 268 103 250 Antisense
CAATTCCTGTTCTGTAATTTTCTCT 417 179 258 Antisense
Sequence Source Affymetrix Proprietary Database