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[172976_s_at]

Description C51734_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C51734 /DEF=C.elegans cDNA clone yk212h6 : 3prime end, single read.
Cluster 4grp_high_8_8 > 32      6grp_total > 05      4grp_total > 47      6grp_cgc > 32      6grp_cgc_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 211
1 2 3 4 range
A 222.2
(3)
P 		47.4
(-2)
P 		30.4
(-2.5)
A 		11.7
(-3)
A 		211
B 150.5
(3)
P 		75.9
(-0.3)
P 		14.3
(-3)
A 		64.5
(-0.8)
P 		136
C 198.3
(3)
P 		42.8
(-3)
A 		56.5
(-2.5)
P 		96.6
(-0.9)
M 		156
avr 190.3
(3) 		55.4
(-2.2) 		33.7
(-3) 		57.6
(-2.1) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 106.1
(2.3)
A 		114.2
(3)
P 		62.1
(-1.5)
P 		59.5
(-1.8)
A 		50.3
(-2.6)
A 		45.2
(-3)
A 		69
B 128.2
(3)
P 		113.5
(2.1)
P 		61.3
(-1.2)
P 		48.7
(-1.9)
P 		64.1
(-1)
P 		31.6
(-3)
A 		97
C 122.7
(3)
P 		93.6
(1.1)
P 		89.4
(0.8)
P 		33.6
(-2.9)
A 		71.5
(-0.4)
P 		32.6
(-3)
A 		90
avr 119
(3) 		107.1
(2.1) 		70.9
(-0.5) 		47.3
(-2.2) 		62
(-1.1) 		36.5
(-3) 		83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 80.9
(3)
P 		10.3
(-2.3)
P 		8
(-2.5)
A 		1.7
(-3)
A 		79
B 48
(3)
P 		18.8
(-0.9)
P 		3.3
(-3)
A 		13.7
(-1.6)
P 		45
C 65.7
(3)
P 		12.3
(-3)
A 		19.7
(-2.2)
P 		16.3
(-2.6)
M 		53
avr 64.9
(3) 		13.8
(-2.6) 		10.3
(-3) 		10.6
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 19.6
(-1.3)
A 		40.6
(3)
P 		13.5
(-2.5)
P 		15.1
(-2.2)
A 		15.2
(-2.1)
A 		11
(-3)
A 		30
B 44.6
(3)
P 		37.8
(1.8)
P 		14.9
(-2.4)
P 		12.9
(-2.8)
P 		26
(-0.4)
P 		11.8
(-3)
A 		33
C 45.5
(3)
P 		28.6
(0.4)
P 		22.7
(-0.5)
P 		6.8
(-3)
A 		23.1
(-0.5)
P 		10.9
(-2.4)
A 		39
avr 36.6
(3) 		35.7
(2.8) 		17
(-1.6) 		11.6
(-2.9) 		21.4
(-0.6) 		11.2
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172976_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26439
Sequence Type Exemplar sequence
Representative Public ID C51734 NCBI
Target Description C51734_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C51734 /DEF=C.elegans cDNA clone yk212h6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026325(11), NM_001026327(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026325 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026327 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
GENEFINDER00000014634 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3999058:4001912:-1 11/11 None
F55C7.7a ENSEMBL cdna:known chromosome:CEL140:I:3999350:4031628:-1 gene:F55C7.7 11/11 A 
F55C7.7c ENSEMBL cdna:known chromosome:CEL140:I:3999350:4020543:-1 gene:F55C7.7 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014626 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171988 6 2 NM_001026325 172976_s_at (11), 186050_s_at (11), 190347_s_at (11), 193621_s_at (11)
NM_001026326 190347_s_at (11)
NM_001026327 172976_s_at (11), 186050_s_at (11), 193621_s_at (11)
NM_001026328 186050_s_at (11)
NM_001026329 186050_s_at (11)
NM_001026330 186050_s_at (9), 190347_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3999386-3999798(-) 88.33 88.33
Public Domain and Genome References
Ensembl F55C7.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71108 Uncoordinated protein 73, isoform a [Caenorhabditis elegans] ref|NP_001021496.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12931.1| guanine nucleotide exchange factor UNC-73A [Caenorhabditis elegans] 0.0
blast AAT68892 Uncoordinated protein 73, isoform f [Caenorhabditis elegans] ref|NP_001021501.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast AAC71109 Uncoordinated protein 73, isoform b [Caenorhabditis elegans] ref|NP_001021497.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12932.1| guanine nucleotide exchange factor UNC-73B [Caenorhabditis elegans] 0.0
blast AAC71110 Uncoordinated protein 73, isoform c [Caenorhabditis elegans] ref|NP_001021498.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast CAE66729 Hypothetical protein CBG12078 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.7E-9
Pfam IPR000219 EMBL-EBI DH domain 2.4E-18
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000014634 2 24-43,50-72
Sequence
Target Sequence 
>C. ELEGANS:172976_S_AT
aagcggtgatcatgaggttcgcctaaaatggaaagcggaatcaggcttgaagtactgcat
tgagtaccgtattttggacgactcatctccagaaaattggctaatcgcgtcgacgaacat
tgagaaaacacatgtttcactgcgaaattttgctcgaaacagttattcattccgagtttt
tgcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCGGTGATCATGAGGTTCGCCTA 537 149 97 Antisense
GAGGTTCGCCTAAAATGGAAAGCGG 382 401 110 Antisense
GCGGAATCAGGCTTGAAGTACTGCA 169 293 131 Antisense
ACTGCATTGAGTACCGTATTTTGGA 581 97 150 Antisense
ACCGTATTTTGGACGACTCATCTCC 557 87 162 Antisense
GAAAATTGGCTAATCGCGTCGACGA 641 351 188 Antisense
GGCTAATCGCGTCGACGAACATTGA 59 539 195 Antisense
TCGCGTCGACGAACATTGAGAAAAC 331 597 201 Antisense
TTCACTGCGAAATTTTGCTCGAAAC 525 687 232 Antisense
TGCTCGAAACAGTTATTCATTCCGA 448 581 247 Antisense
GTTATTCATTCCGAGTTTTTGCATA 555 447 258 Antisense
Sequence Source Affymetrix Proprietary Database