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[172973_at] ADP-Ribosyltransferase

Description C58093_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C58093 /DEF=C.elegans cDNA clone yk361b1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 89      4grp_high_8_8 > 08      6grp_highest_nonorm > 92      6grp_total > 98      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 69      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 332.5
(3)
P 		247.2
(-1.6)
P 		220.6
(-3)
P 		293.7
(0.9)
M 		112
B 383.8
(2.3)
P 		225.1
(-3)
P 		282
(-1.1)
P 		405.8
(3)
P 		181
C 350
(3)
P 		275.5
(-0.7)
P 		324.5
(1.7)
P 		228.2
(-3)
P 		122
avr 355.4
(3) 		249.3
(-3) 		275.7
(-1.5) 		309.2
(0.4) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 369
1 2 3 4 5 6 range
A 423.1
(2.2)
P 		244.2
(-3)
P 		243.9
(-3)
P 		253.1
(-2.7)
P 		436.1
(2.6)
P 		451.4
(3)
P 		208
B 285.6
(-1.5)
P 		267.6
(-1.8)
P 		192.2
(-3)
P 		371.8
(-0.1)
P 		401
(0.4)
P 		560.7
(3)
P 		369
C 331.6
(0.2)
P 		220
(-3)
P 		257.5
(-1.9)
P 		380.2
(1.6)
P 		391
(1.9)
P 		429.4
(3)
P 		209
avr 346.8
(-0.2) 		243.9
(-2.7) 		231.2
(-3) 		335
(-0.5) 		409.4
(1.3) 		480.5
(3) 		249
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 121.1
(3)
P 		53.7
(-2.2)
P 		57.7
(-1.9)
P 		43
(-3)
M 		78
B 122.4
(3)
P 		55.6
(-3)
P 		65.3
(-2.1)
P 		86.5
(-0.2)
P 		67
C 115.9
(3)
P 		79.2
(0.2)
P 		113.1
(2.8)
P 		38.5
(-3)
P 		77
avr 119.8
(3) 		62.8
(-2.4) 		78.7
(-0.9) 		56
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 78
(-1.1)
P 		86.9
(-0.4)
P 		53.1
(-3)
P 		64.2
(-2.2)
P 		131.6
(3)
P 		109.9
(1.3)
P 		79
B 99.4
(-1.1)
P 		89.1
(-1.4)
P 		46.7
(-3)
P 		98.5
(-1.1)
P 		162.9
(1.3)
P 		209.1
(3)
P 		162
C 122.9
(1.4)
P 		67.1
(-2.9)
P 		65.5
(-3)
P 		76.5
(-2.2)
P 		126.5
(1.7)
P 		143.7
(3)
P 		78
avr 100.1
(-0.3) 		81
(-1.4) 		55.1
(-3) 		79.7
(-1.5) 		140.3
(2.2) 		154.2
(3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172973_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26603
Sequence Type Exemplar sequence
Representative Public ID C58093 NCBI
Target Description C58093_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C58093 /DEF=C.elegans cDNA clone yk361b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026886(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026886 NCBI Caenorhabditis elegans E02H1.4 (ADP-Ribosyltransferase) mRNA, complete cds. 11/11 None
E02H1.4 ENSEMBL cdna:known chromosome:CEL140:II:9592947:9595040:1 gene:E02H1.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9594708-9594969(+) 87.0 87.0
Public Domain and Genome References
Gene Title ADP-Ribosyltransferase
Gene Symbol pme-2
Ensembl E02H1.4
Entrez Gene 3565967 Entrez gene
SwissProt Q09525 EMBL-EBI
WormBase CE01539 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6471 protein amino acid ADP-ribosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3950 NAD+ ADP-ribosyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87379 Hypothetical protein E02H1.4 [Caenorhabditis elegans] ref|NP_001022057.1| E02H1.4 [Caenorhabditis elegans] gb|AAM27196.1| poly ADP-ribose metabolism enzyme-2 [Caenorhabditis elegans] sp|Q09525|PME2_CAEEL Poly(ADP-ribose) polymerase pme-2 (Poly ADP-ribose metabolism enzyme 2) 0.0
blast CAE57909 Hypothetical protein CBG00959 [Caenorhabditis briggsae] 0.0
blast XP_520665 PREDICTED: similar to poly-(ADP-ribose) polymerase II [Pan troglodytes] 5.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR001290 EMBL-EBI Poly(ADP-ribose) polymerase, catalytic region 1.0E-126
Pfam IPR008893 EMBL-EBI WGR 4.6E-36
Pfam IPR004102 EMBL-EBI Poly(ADP-ribose) polymerase, regulatory domain 5.6E-77
Sequence
Target Sequence 
>C. ELEGANS:172973_AT
gatggcttaaatgctaatttctcatgtaatctctcacattttatctgtttctacctctcc
atttaattttctgatctgaatttgtcaagcttttgtaactttttttaaacctgttaatcc
aatgatactcctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGCTTAAATGCTAATTTCTCAT 289 407 135 Antisense
GCTAATTTCTCATGTAATCTCTCAC 50 303 147 Antisense
ATTTCTCATGTAATCTCTCACATTT 244 17 151 Antisense
AATCTCTCACATTTTATCTGTTTCT 73 171 162 Antisense
CACATTTTATCTGTTTCTACCTCTC 225 197 169 Antisense
TTCTACCTCTCCATTTAATTTTCTG 464 691 183 Antisense
TACCTCTCCATTTAATTTTCTGATC 669 649 186 Antisense
TCTCCATTTAATTTTCTGATCTGAA 215 611 190 Antisense
TTCTGATCTGAATTTGTCAAGCTTT 679 693 203 Antisense
GAATTTGTCAAGCTTTTGTAACTTT 644 323 212 Antisense
ACCTGTTAATCCAATGATACTCCTC 580 85 243 Antisense
Sequence Source Affymetrix Proprietary Database