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[172962_at] guanine nucleotide-binding protein gamma subunit

Description K02A4.2 /REP_DB=WormBase Gene ID /WP=CE03458 /GEN=gpc-1 /TR=SW:P54406 /GB=CAA91806.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein gamma subunit
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 70      6grp_total > 14      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 384
1 2 3 4 range
A 496.4
(3)
P 		268.6
(-0.9)
P 		167.1
(-2.7)
P 		147.9
(-3)
P 		349
B 426.9
(3)
P 		228
(-1.5)
P 		163.7
(-3)
P 		201.6
(-2.1)
P 		263
C 531.7
(3)
P 		218.7
(-2.5)
P 		192.7
(-3)
P 		291.5
(-1.3)
P 		339
avr 485
(3) 		238.4
(-1.8) 		174.5
(-3) 		213.7
(-2.2) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 394
1 2 3 4 5 6 range
A 404.3
(3)
P 		334.1
(1.4)
P 		286.8
(0.4)
P 		208
(-1.3)
P 		150.7
(-2.6)
P 		132.9
(-3)
P 		271
B 442.4
(3)
P 		318.1
(0.8)
P 		269.6
(-0.1)
P 		212.7
(-1.1)
P 		173.8
(-1.8)
P 		105.1
(-3)
P 		337
C 498.7
(3)
P 		378.3
(1.1)
P 		183.8
(-1.9)
P 		231.7
(-1.1)
P 		192.6
(-1.7)
P 		111.6
(-3)
P 		387
avr 448.5
(3) 		343.5
(1.1) 		246.7
(-0.6) 		217.5
(-1.2) 		172.4
(-2) 		116.5
(-3) 		332
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 159
1 2 3 4 range
A 180.7
(3)
P 		58.3
(-1.6)
P 		43.7
(-2.2)
P 		21.7
(-3)
P 		159
B 136.1
(3)
P 		56.3
(-1.9)
P 		37.9
(-3)
P 		43
(-2.7)
P 		98
C 176
(3)
P 		62.9
(-2.4)
P 		67.1
(-2.2)
P 		49.2
(-3)
P 		127
avr 164.3
(3) 		59.2
(-2) 		49.6
(-2.4) 		38
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 74.5
(-0.1)
P 		118.8
(3)
P 		62.5
(-0.9)
P 		52.8
(-1.6)
P 		45.5
(-2.1)
P 		32.3
(-3)
P 		87
B 153.9
(3)
P 		105.9
(0.5)
P 		65.5
(-1.6)
P 		56.3
(-2.1)
P 		70.6
(-1.4)
P 		39.2
(-3)
P 		115
C 184.8
(3)
P 		115.4
(0.2)
P 		46.8
(-2.6)
P 		46.6
(-2.6)
P 		62.3
(-2)
P 		37.4
(-3)
P 		147
avr 137.7
(3) 		113.4
(1.6) 		58.3
(-1.7) 		51.9
(-2.1) 		59.5
(-1.6) 		36.3
(-3) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172962_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17498
Sequence Type Exemplar sequence
Representative Public ID K02A4.2 NCBI
Target Description K02A4.2 /REP_DB=WormBase Gene ID /WP=CE03458 /GEN=gpc-1 /TR=SW:P54406 /GB=CAA91806.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein gamma subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077744(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077744 NCBI Caenorhabditis elegans G Protein, Gamma subunit family member (gpc-1) (gpc-1) mRNA, complete cds. 11/11 None
SNAP00000020681 ENSEMBL cdna:SNAP chromosome:CEL140:X:12881509:12882330:-1 11/11 None
GENEFINDER00000020682 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12881509:12882330:-1 11/11 None
K02A4.2 ENSEMBL cdna:known chromosome:CEL140:X:12881147:12882627:-1 gene:K02A4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12881508-12882330(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein gamma subunit
Gene Symbol gpc-1
Ensembl K02A4.2
Entrez Gene 181424 Entrez gene
SwissProt P54406 EMBL-EBI
WormBase CE03458 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
7186 G-protein coupled receptor protein signaling pathway inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5834 heterotrimeric G-protein complex inferred from electronic annotation QuickGO AmiGO
5834 heterotrimeric G-protein complex inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
3924 GTPase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91806 Hypothetical protein K02A4.2 [Caenorhabditis elegans] ref|NP_510145.1| G Protein, Gamma subunit family member (gpc-1) [Caenorhabditis elegans] gb|AAK55965.1| heterotrimeric G protein gamma subunit 1 [Caenorhabditis elegans] sp|P54406|GBG_CAEEL Guanine nucleotide-binding protein gamma subunit 7.0E-29
blast AAW02910 gpc-1 [Caenorhabditis briggsae] sp|Q4VT26|GBG_CAEBR Guanine nucleotide-binding protein gamma subunit 5.0E-22
blast CAE57199 Hypothetical protein CBG00049 [Caenorhabditis briggsae] 5.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 7.7E-29
Sequence
Target Sequence 
>C. ELEGANS:172962_AT
ggaaaacatcaaggcatcaaccgaacaactctgtgctgaggccaacattcaacgaaaaaa
agtttccgaagtttcaaaggaactgctcgatttttgcgagaagaacaagaccaacgacat
gctggtctccgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAAACATCAAGGCATCAACCGAA 365 527 15 Antisense
CAAGGCATCAACCGAACAACTCTGT 264 183 24 Antisense
ATCAACCGAACAACTCTGTGCTGAG 187 27 30 Antisense
GAACAACTCTGTGCTGAGGCCAACA 259 353 37 Antisense
AACTCTGTGCTGAGGCCAACATTCA 334 141 41 Antisense
CTGTGCTGAGGCCAACATTCAACGA 616 239 45 Antisense
GCTGAGGCCAACATTCAACGAAAAA 32 293 49 Antisense
CAAAGGAACTGCTCGATTTTTGCGA 634 191 89 Antisense
GGAACTGCTCGATTTTTGCGAGAAG 33 531 93 Antisense
GAAGAACAAGACCAACGACATGCTG 410 339 114 Antisense
AGACCAACGACATGCTGGTCTCCGG 677 69 122 Antisense
Sequence Source Affymetrix Proprietary Database