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[172949_s_at]

Description C51933_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C51933 /DEF=C.elegans cDNA clone yk225c2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 73      6grp_total > 36      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 589.4
(3)
P 		405.2
(-2.1)
P 		388.3
(-2.5)
P 		371.7
(-3)
P 		218
B 507.3
(3)
P 		325
(-3)
P 		349.1
(-2.2)
P 		377.4
(-1.3)
P 		182
C 613
(3)
P 		335.8
(-3)
P 		420.5
(-1.2)
P 		375.9
(-2.1)
P 		277
avr 569.9
(3) 		355.3
(-3) 		386
(-2.1) 		375
(-2.4) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 445.5
(-0)
P 		533.6
(3)
P 		360.1
(-3)
P 		485.5
(1.3)
P 		399.2
(-1.6)
P 		480
(1.1)
P 		174
B 672.6
(3)
P 		629.6
(2)
P 		602.9
(1.3)
P 		420.7
(-3)
P 		433.4
(-2.7)
P 		599.5
(1.3)
P 		252
C 584.6
(3)
P 		517.6
(0)
P 		535.2
(0.8)
P 		496.5
(-0.9)
P 		525.9
(0.4)
P 		448.9
(-3)
P 		136
avr 567.6
(3) 		560.3
(2.6) 		499.4
(-0.6) 		467.6
(-2.2) 		452.8
(-3) 		509.5
(-0) 		115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 214.6
(3)
P 		87.9
(-1.7)
P 		101.5
(-1.2)
P 		54.4
(-3)
P 		160
B 161.8
(3)
P 		80.3
(-3)
P 		80.9
(-3)
P 		80.4
(-3)
P 		82
C 202.9
(3)
P 		96.6
(-1.6)
P 		146.6
(0.6)
P 		63.4
(-3)
P 		140
avr 193.1
(3) 		88.3
(-2) 		109.7
(-0.9) 		66.1
(-3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 82.1
(-2.8)
P 		189.8
(3)
P 		78.4
(-3)
P 		123.1
(-0.6)
P 		120.5
(-0.7)
P 		116.9
(-0.9)
P 		111
B 234
(3)
P 		209.6
(1.8)
P 		146.4
(-1.3)
P 		111.4
(-3)
P 		176.1
(0.2)
P 		223.5
(2.5)
P 		123
C 216.7
(3)
P 		157.9
(-0)
P 		136.2
(-1.1)
P 		99.8
(-3)
P 		170.2
(0.6)
P 		150.3
(-0.4)
P 		117
avr 177.6
(2.3) 		185.8
(3) 		120.3
(-2.3) 		111.4
(-3) 		155.6
(0.6) 		163.6
(1.2) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172949_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26444
Sequence Type Exemplar sequence
Representative Public ID C51933 NCBI
Target Description C51933_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=C51933 /DEF=C.elegans cDNA clone yk225c2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062939(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062939 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-8) (dnj-8) mRNA, complete cds. 11/11 A 
SNAP00000022068 ENSEMBL cdna:SNAP chromosome:CEL140:II:6594643:6598015:-1 11/11 None
GENEFINDER00000022077 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6594643:6598015:-1 11/11 None
C56C10.13 ENSEMBL cdna:known chromosome:CEL140:II:6594335:6598015:-1 gene:C56C10.13 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6594897-6595554(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-8
Ensembl C56C10.13
Entrez Gene 174093 Entrez gene
SwissProt Q95QQ1 EMBL-EBI
WormBase CE28901 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18042.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 16 cfa
CANINE_2:CFAAFFX.24923.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 16 cfa
CHICKEN:GGAAFFX.12043.1.S1_S_AT similar to KIAA0962 protein gga
HG-U133_PLUS_2:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBA:RC_AA258203_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBC:RC_H80637_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.9059.0.S2_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.9059.0.S1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.272327.0.S1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U95AV2:33170_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:242947_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133B:242947_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U95D:91976_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBC:RC_D11881_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
MG-U74BV2:105676_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MOE430B:1436209_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MOUSE430_2:1436209_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MU19KSUBC:TC39745_AT MKIAA0962 protein mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK93843 Dnaj domain (prokaryotic heat shock protein) protein 8 [Caenorhabditis elegans] ref|NP_495340.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-8) [Caenorhabditis elegans] 0.0
blast T15851 hypothetical protein C56C10.11 - Caenorhabditis elegans 0.0
blast CAE60663 Hypothetical protein CBG04316 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 4.00000007816593E-24
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.6E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495340.1 1 526-548
C56C10.13 1 526-548
SNAP00000022068 1 526-548
GENEFINDER00000022077 1 526-548
Sequence
Target Sequence 
>C. ELEGANS:172949_S_AT
ctcacaatatgtgtatcctctcagaaacaacaaaacattctcattcggatttctgatagt
tccaaaaaatctggattggttccgtaaactactggagcatacacttccaactgatggaaa
accaacgcctgaaccagatgttagtcaatcaatgtataaaagactgaaattaatcaacca
tcgacatactattggaacagttttgacactatgcggttggaagctctatttctcaattta
ccatccaaaacacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCACAATATGTGTATCCTCTCAGA 638 227 24 Antisense
CAAAACATTCTCATTCGGATTTCTG 405 191 54 Antisense
TTCGGATTTCTGATAGTTCCAAAAA 356 693 67 Antisense
GGTTCCGTAAACTACTGGAGCATAC 406 507 101 Antisense
GGAGCATACACTTCCAACTGATGGA 23 521 117 Antisense
GAAAACCAACGCCTGAACCAGATGT 89 355 140 Antisense
AATTAATCAACCATCGACATACTAT 182 175 191 Antisense
GGAACAGTTTTGACACTATGCGGTT 382 529 217 Antisense
ATGCGGTTGGAAGCTCTATTTCTCA 47 41 234 Antisense
GCTCTATTTCTCAATTTACCATCCA 183 301 246 Antisense
CTCAATTTACCATCCAAAACACGTG 526 227 255 Antisense
Sequence Source Affymetrix Proprietary Database