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[172932_x_at]

Description ZC101.2A /REP_DB=WormBase Gene ID /WP=CE15028 /GEN=unc-52 /TR=O18263 /GB=CAB07706.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=UNC-52 membrane proteoglycan
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 12      6grp_total > 12      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 62      6grp_high1_sub > De      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2930
1 2 3 4 range
A 1705.4
(0.1)
P 		3025.3
(2.8)
P 		3102.1
(3)
P 		256.4
(-3)
P 		2846
B 1689.4
(0.3)
P 		2963.8
(3)
P 		2752.7
(2.5)
P 		183.2
(-3)
P 		2781
C 1742.3
(0.5)
P 		2896.5
(3)
P 		2695
(2.6)
P 		171.9
(-3)
P 		2725
avr 1712.4
(0.3) 		2961.9
(3) 		2849.9
(2.8) 		203.8
(-3) 		2758

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2278
1 2 3 4 5 6 range
A 2423.8
(1.9)
P 		2809.9
(3)
P 		1656.4
(-0.3)
P 		1507.3
(-0.7)
P 		1302.3
(-1.3)
P 		697.1
(-3)
P 		2113
B 1820.9
(0.1)
P 		2905.6
(3)
P 		2145.9
(1)
P 		1619.4
(-0.5)
P 		994
(-2.1)
P 		671.2
(-3)
P 		2234
C 2439.5
(2.1)
P 		2740.7
(3)
P 		2321
(1.8)
P 		1203.8
(-1.4)
P 		1489.4
(-0.6)
P 		627.9
(-3)
P 		2113
avr 2228.1
(1.4) 		2818.7
(3) 		2041.1
(0.8) 		1443.5
(-0.8) 		1261.9
(-1.3) 		665.4
(-3) 		2153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 910
1 2 3 4 range
A 620.9
(1.5)
P 		656.7
(1.8)
P 		811
(3)
P 		37.5
(-3)
P 		774
B 538.7
(1.3)
P 		732
(3)
P 		637.9
(2.2)
P 		39
(-3)
P 		693
C 576.7
(0.6)
P 		832.8
(2.3)
P 		939.3
(3)
P 		29
(-3)
P 		910
avr 578.8
(1.3) 		740.5
(2.6) 		796.1
(3) 		35.2
(-3) 		761

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 830
1 2 3 4 5 6 range
A 446.8
(-1)
P 		999.3
(3)
P 		360.7
(-1.6)
P 		382.3
(-1.5)
P 		393
(-1.4)
P 		169.7
(-3)
P 		830
B 633.6
(0.2)
P 		967.1
(3)
P 		521.1
(-0.7)
P 		428.9
(-1.5)
P 		403.8
(-1.7)
P 		250.3
(-3)
P 		717
C 904.2
(3)
P 		836.3
(2.4)
P 		590.7
(0.3)
P 		242.1
(-2.7)
P 		481.9
(-0.7)
P 		210.2
(-3)
P 		694
avr 661.5
(0.7) 		934.2
(3) 		490.8
(-0.7) 		351.1
(-1.8) 		426.2
(-1.2) 		210.1
(-3) 		724
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172932_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3605
Sequence Type Exemplar sequence
Representative Public ID ZC101.2A NCBI
Target Description ZC101.2A /REP_DB=WormBase Gene ID /WP=CE15028 /GEN=unc-52 /TR=O18263 /GB=CAB07706.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=UNC-52 membrane proteoglycan
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064643(10), NM_001027317(11), NM_064644(11), NM_064645(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064643 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 10/11 None
NM_001027317 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 None
NM_064644 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 A 
NM_064645 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 A 
ZC101.2e ENSEMBL cdna:known chromosome:CEL140:II:14647215:14666743:-1 gene:ZC101.2 10/11 A 
ZC101.2f ENSEMBL cdna:known chromosome:CEL140:II:14652052:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2a ENSEMBL cdna:known chromosome:CEL140:II:14652449:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2c ENSEMBL cdna:known chromosome:CEL140:II:14652449:14666743:-1 gene:ZC101.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013249 3/11 Cross Hyb Matching Probes None
GENEFINDER00000013270 3/11 Cross Hyb Matching Probes None
SNAP00000016884 7/11 Cross Hyb Matching Probes None
GENEFINDER00000016886 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175126 5 4 NM_001027317 172932_x_at (11)
NM_064643 172932_x_at (10), 194141_at (11)
NM_064644 172932_x_at (11)
NM_064645 172932_x_at (11)
NM_064646 174785_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14652445-14666740(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC101.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07708 Hypothetical protein ZC101.2e [Caenorhabditis elegans] emb|CAB07569.1| Hypothetical protein ZC101.2e [Caenorhabditis elegans] ref|NP_497044.3| UNCoordinated family member (unc-52) [Caenorhabditis elegans] sp|Q06561|UNC52_CAEEL Basement membrane proteoglycan precursor (Perlecan homolog) (Uncoordinated protein 52) pir||T19821 hypothetical protein ZC101.2e - Caenorhabditis elegans 0.0
blast CAB07706 Hypothetical protein ZC101.2a [Caenorhabditis elegans] emb|CAB07567.1| Hypothetical protein ZC101.2a [Caenorhabditis elegans] ref|NP_497046.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] 0.0
blast AAA28156 basement membrane proteoglycan 0.0
blast CAH04744 Hypothetical protein ZC101.2f [Caenorhabditis elegans] emb|CAH04704.1| Hypothetical protein ZC101.2f [Caenorhabditis elegans] ref|NP_001022488.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] 0.0
blast CAB07707 Hypothetical protein ZC101.2c [Caenorhabditis elegans] emb|CAB07568.1| Hypothetical protein ZC101.2c [Caenorhabditis elegans] ref|NP_497045.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] pir||C88369 protein unc-52 [imported] - Caenorhabditis elegans 0.0
blast CAE65897 Hypothetical protein CBG11064 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 1.2E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 7.6E-4
Pfam IPR001791 EMBL-EBI Laminin G 5.0E-5
Pfam IPR001791 EMBL-EBI Laminin G 5.3E-33
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-18
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.1E-20
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 8.1E-20
Pfam IPR000034 EMBL-EBI Laminin B 9.3E-79
Pfam IPR000034 EMBL-EBI Laminin B 5.3E-73
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 7.8E-20
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.7E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0023
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.6E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Sequence
Target Sequence 
>C. ELEGANS:172932_X_AT
acgataaatccgatgcgtccttcacctgcccaatctactcggtaccaggatcaaaggtcg
actggacctacgaaaacggagatcttccatccaaggcagttccaaatggcaacaagattg
agatcaaggaattcgacgatgcatctgctggaacctatgtttgcaaggtttcgttcgatg
gaaacgttgttgaaggatttgtgaccgctcaaatgtttgtacctgatactattatccaag
tgcttctcgaagtttcctctgaatcaccacaaatcggggatcgtgcctggtttgactgta
aggtaactggagacccatcggctgtgatctcatggaccaaagaaggaaatgatgatcttc
caccaaatgctcaggtaaccggaggacgtctcttgttcactgatctcaaggaagacaacg
ctggcgtctacagatgcgtcgccaagacaaaagcgggaccacttcaaacacgaacagtgc
tcaacgtcggatccggaaaacgcaagcgaaaacacttgggaaatcgtcgggggcgtcgtt
tgaggcatcgtagacgcaacgctcaaaatgggccactttctcgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGATAAATCCGATGCGTCCTTCAC 497 93 6836 Antisense
ATCTTCCATCCAAGGCAGTTCCAAA 221 31 6917 Antisense
GGAATTCGACGATGCATCTGCTGGA 686 317 6963 Antisense
TGCTTCTCGAAGTTTCCTCTGAATC 412 579 7076 Antisense
GGATCGTGCCTGGTTTGACTGTAAG 249 511 7113 Antisense
TAACTGGAGACCCATCGGCTGTGAT 360 639 7139 Antisense
AATGATGATCTTCCACCAAATGCTC 658 163 7183 Antisense
TAACCGGAGGACGTCTCTTGTTCAC 465 633 7211 Antisense
ACGCTGGCGTCTACAGATGCGTCGC 1 93 7253 Antisense
CGAACAGTGCTCAACGTCGGATCCG 198 253 7306 Antisense
GCTCAAAATGGGCCACTTTCTCGCA 710 301 7396 Antisense
Sequence Source Affymetrix Proprietary Database