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[172914_x_at]

Description F31B12.1A /REP_DB=WormBase Gene ID /WP=CE26713 /GEN=plc-1 /TR=Q9TVI2 /GB=CAB60298.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=phosphoinositide-specific phospholipase C
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 51      6grp_total > 26      4grp_total > 30      6grp_highest_sub > 01_02      6grp_cgc > 23      6grp_high1_sub > 01_02      6grp_cgc_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 720
1 2 3 4 range
A 943.3
(3)
P 		361.8
(-2.9)
P 		353.5
(-3)
P 		717.5
(0.7)
P 		590
B 870.6
(3)
P 		408
(-1.3)
P 		223.3
(-3)
P 		652.6
(1)
P 		647
C 715.4
(3)
P 		383.6
(-2.3)
P 		337.7
(-3)
P 		614
(1.4)
P 		378
avr 843.1
(3) 		384.5
(-2.1) 		304.8
(-3) 		661.4
(1) 		538

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 652
1 2 3 4 5 6 range
A 824.3
(3)
P 		690.2
(1.5)
P 		271
(-3)
P 		515.8
(-0.3)
P 		507.8
(-0.4)
P 		467.3
(-0.9)
P 		553
B 700.4
(3)
P 		618.6
(1.4)
P 		460.2
(-1.7)
P 		508
(-0.7)
P 		391.7
(-3)
P 		435.7
(-2.1)
P 		309
C 641.8
(-0.1)
P 		922.8
(3)
P 		594.3
(-0.7)
P 		468.8
(-2.1)
P 		387
(-3)
P 		441.4
(-2.4)
P 		536
avr 722.2
(2.6) 		743.9
(3) 		441.8
(-2.8) 		497.5
(-1.7) 		428.8
(-3) 		448.1
(-2.6) 		315
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 343.5
(3)
P 		78.5
(-3)
P 		92.4
(-2.7)
P 		105
(-2.4)
P 		265
B 277.6
(3)
P 		100.8
(-1.7)
P 		51.7
(-3)
P 		139.1
(-0.7)
P 		226
C 236.8
(3)
P 		110.3
(-2.7)
P 		117.7
(-2.4)
P 		103.5
(-3)
P 		133
avr 286
(3) 		96.5
(-2.7) 		87.3
(-3) 		115.9
(-2.1) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 152
(-0)
P 		245.5
(3)
P 		59
(-3)
P 		130.8
(-0.7)
P 		153.2
(0)
P 		113.8
(-1.2)
P 		187
B 243.7
(3)
P 		205.9
(1.3)
P 		111.7
(-3)
P 		134.5
(-2)
P 		159.2
(-0.8)
P 		162.5
(-0.7)
P 		132
C 237.9
(1.6)
P 		281.6
(3)
P 		151.3
(-1.2)
P 		94.3
(-3)
P 		125.2
(-2)
P 		147.8
(-1.3)
P 		187
avr 211.2
(1.6) 		244.3
(3) 		107.3
(-3) 		119.9
(-2.4) 		145.9
(-1.3) 		141.4
(-1.5) 		137
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172914_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17028
Sequence Type Exemplar sequence
Representative Public ID F31B12.1A NCBI
Target Description F31B12.1A /REP_DB=WormBase Gene ID /WP=CE26713 /GEN=plc-1 /TR=Q9TVI2 /GB=CAB60298.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=phosphoinositide-specific phospholipase C
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029448(10), NM_001029446(11), NM_077404(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029448 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-1) (plc-1) mRNA, complete cds. 10/11 None
NM_001029446 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-1) (plc-1) mRNA, complete cds. 11/11 None
NM_077404 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-1) (plc-1) mRNA, complete cds. 10/11 A 
SNAP00000024685 ENSEMBL cdna:SNAP chromosome:CEL140:X:10796433:10801713:-1 11/11 None
GENEFINDER00000024691 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10796773:10800828:-1 9/11 None
F31B12.1c ENSEMBL cdna:known chromosome:CEL140:X:10794423:10812457:-1 gene:F31B12.1 10/11 A 
F31B12.1a ENSEMBL cdna:known chromosome:CEL140:X:10795670:10812495:-1 gene:F31B12.1 11/11 A 
F31B12.1b ENSEMBL cdna:known chromosome:CEL140:X:10796082:10812457:-1 gene:F31B12.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10796369-10812457(-) 100.0 100.0
Public Domain and Genome References
Ensembl F31B12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD44153 Hypothetical protein F31B12.1c [Caenorhabditis elegans] emb|CAD44124.1| Hypothetical protein F31B12.1c [Caenorhabditis elegans] ref|NP_001024619.1| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] 0.0
blast CAB60299 Hypothetical protein F31B12.1b [Caenorhabditis elegans] emb|CAB60282.4| Hypothetical protein F31B12.1b [Caenorhabditis elegans] ref|NP_509805.3| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] 0.0
blast CAB60298 Hypothetical protein F31B12.1a [Caenorhabditis elegans] emb|CAB60281.3| Hypothetical protein F31B12.1a [Caenorhabditis elegans] ref|NP_001024617.1| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] gb|AAC38963.1| phospholipase C PLC210 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000159 EMBL-EBI RA domain 1.0E-19
Pfam IPR000159 EMBL-EBI RA domain 6.5E-22
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 2.5E-68
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 9.0E-35
Pfam IPR000008 EMBL-EBI C2 domain 2.0E-14
Pfam IPR000159 EMBL-EBI RA domain 9.6E-20
Pfam IPR000159 EMBL-EBI RA domain 6.5E-22
Pfam IPR000159 EMBL-EBI RA domain 1.0E-19
Pfam IPR000159 EMBL-EBI RA domain 9.6E-20
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 2.1E-43
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 9.0E-35
Pfam IPR000008 EMBL-EBI C2 domain 2.0E-14
Pfam IPR000159 EMBL-EBI RA domain 1.0E-19
Pfam IPR000159 EMBL-EBI RA domain 5.4E-10
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 9.0E-35
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-11
Sequence
Target Sequence 
>C. ELEGANS:172914_X_AT
ggacgatcattggacactgacgcttttggagagcaccttgaagtgacagaaggcaagtgt
aaggatactaacagatcgggtcatgataaaactaaatgtgtgatatttgtagggttgaac
cctcgtgcaagatcaatgggcgacacatttctggtatgtgttcacaatgttagtgaagat
cagccttatgcaatcctacgtgctggaatccacagcactgccgccgacattattcgtcag
gtgtttgttaaagctcgccgttcaaatgtagacgattccgagtttgttcttgttgaagaa
acctgcgatgatccaaaactcaatcaaggccaaagtatgctacaagccctttcgttggcg
cggaaaagaagcaatgatctgactccaaagtatccaaacaaccgcaccacaagccgagtg
ttaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACGATCATTGGACACTGACGCTT 219 519 5167 Antisense
GACGCTTTTGGAGAGCACCTTGAAG 222 381 5185 Antisense
GGTTGAACCCTCGTGCAAGATCAAT 629 503 5279 Antisense
GAAGATCAGCCTTATGCAATCCTAC 560 337 5341 Antisense
GCAATCCTACGTGCTGGAATCCACA 102 321 5356 Antisense
TGCCGCCGACATTATTCGTCAGGTG 350 585 5385 Antisense
GTGTTTGTTAAAGCTCGCCGTTCAA 235 453 5407 Antisense
GACGATTCCGAGTTTGTTCTTGTTG 291 379 5437 Antisense
GTATGCTACAAGCCCTTTCGTTGGC 323 453 5501 Antisense
CCAAAGTATCCAAACAACCGCACCA 380 275 5551 Antisense
CGCACCACAAGCCGAGTGTTAGGAC 202 255 5569 Antisense
Sequence Source Affymetrix Proprietary Database