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[172904_x_at] Major sperm protein

Description K05F1.7 /REP_DB=WormBase Gene ID /WP=CE02811 /GEN=msp-63 /TR=Q21244 /GB=AAA68714.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster 6grp_total > 99      4grp_total > 17      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 72.8
(-2.5)
A 		66.7
(-3)
A 		116.7
(0.9)
A 		143
(3)
A 		76
B 83.1
(-0.3)
A 		100
(3)
A 		69
(-3)
A 		90.9
(1.2)
A 		31
C 72.1
(-3)
A 		97.3
(-1)
A 		71.6
(-3)
A 		148.1
(3)
A 		77
avr 76
(-3) 		88
(-1.6) 		85.8
(-1.9) 		127.3
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3224
1 2 3 4 5 6 range
A 104.9
(-2.9)
A 		70.7
(-3)
A 		106.1
(-2.9)
P 		225.3
(-2.7)
P 		2284.7
(1.4)
P 		3102.7
(3)
P 		3032
B 94.3
(-2.9)
A 		93.2
(-2.9)
A 		66.2
(-3)
A 		193.9
(-2.7)
P 		2416
(3)
P 		2422.7
(3)
P 		2357
C 117.4
(-2.9)
A 		140.1
(-2.8)
P 		40.9
(-3)
A 		250.7
(-2.6)
P 		3264.9
(3)
P 		2770
(2.1)
P 		3224
avr 105.5
(-2.9) 		101.3
(-2.9) 		71.1
(-3) 		223.3
(-2.7) 		2655.2
(2.8) 		2765.1
(3) 		2694
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 26.5
(1.5)
A 		14.5
(-3)
A 		30.5
(3)
A 		20.9
(-0.6)
A 		16
B 26.5
(3)
A 		24.7
(2)
A 		16
(-3)
A 		19.4
(-1.1)
A 		11
C 23.9
(-3)
A 		28
(3)
A 		24.9
(-1.5)
A 		25
(-1.4)
A 		4
avr 25.6
(3) 		22.4
(-2.1) 		23.8
(0.2) 		21.8
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1046
1 2 3 4 5 6 range
A 19.3
(-3)
A 		25.2
(-3)
A 		23.1
(-3)
P 		57.1
(-2.7)
P 		689.4
(2.5)
P 		755.4
(3)
P 		736
B 32.8
(-2.9)
A 		31
(-2.9)
A 		16.1
(-3)
A 		51.4
(-2.8)
P 		981.6
(3)
P 		903.4
(2.5)
P 		966
C 43.5
(-2.8)
A 		42.7
(-2.8)
P 		10.4
(-3)
A 		50.4
(-2.8)
P 		1056.5
(3)
P 		927.3
(2.3)
P 		1046
avr 31.9
(-2.9) 		33
(-2.9) 		16.5
(-3) 		53
(-2.8) 		909.2
(3) 		862
(2.7) 		893
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172904_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5375
Sequence Type Exemplar sequence
Representative Public ID K05F1.7 NCBI
Target Description K05F1.7 /REP_DB=WormBase Gene ID /WP=CE02811 /GEN=msp-63 /TR=Q21244 /GB=AAA68714.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062742(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062742 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-63) (msp-63) mRNA, complete cds. 11/11 None
SNAP00000031244 ENSEMBL cdna:SNAP chromosome:CEL140:II:5797030:5797413:-1 11/11 None
GENEFINDER00000031253 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5797030:5797413:-1 11/11 None
K05F1.7 ENSEMBL cdna:known chromosome:CEL140:II:5797030:5797413:-1 gene:K05F1.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062569 1/11 Cross Hyb Matching Probes A 
NM_062743 4/11 Cross Hyb Matching Probes A 
NM_062748 5/11 Cross Hyb Matching Probes A 
NM_062753 2/11 Cross Hyb Matching Probes A 
NM_062764 2/11 Cross Hyb Matching Probes A 
SNAP00000021327 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021348 1/11 Cross Hyb Matching Probes None
SNAP00000031245 4/11 Cross Hyb Matching Probes None
GENEFINDER00000031258 4/11 Cross Hyb Matching Probes None
SNAP00000041369 5/11 Cross Hyb Matching Probes None
SNAP00000041374 2/11 Cross Hyb Matching Probes None
SNAP00000041384 2/11 Cross Hyb Matching Probes None
GENEFINDER00000041386 2/11 Cross Hyb Matching Probes None
GENEFINDER00000041391 2/11 Cross Hyb Matching Probes None
GENEFINDER00000041398 5/11 Cross Hyb Matching Probes None
SNAP00000004243 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004263 1/11 Cross Hyb Matching Probes None
C34F11.6 1/11 Cross Hyb Matching Probes A 
K05F1.2 4/11 Cross Hyb Matching Probes A 
ZK1248.6 5/11 Cross Hyb Matching Probes A 
ZK1248.4 2/11 Cross Hyb Matching Probes A 
ZK1248.17 2/11 Cross Hyb Matching Probes A 
K07F5.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5797888-5798268(+) 93.49 93.49
chrII:5797026-5797410(-) 100.0 100.0
chrII:5809908-5810292(-) 95.05 95.05
Public Domain and Genome References
Gene Title Major sperm protein
Gene Symbol msp-63
Ensembl K05F1.7
Entrez Gene 173980 Entrez gene
SwissProt Q21244 EMBL-EBI
WormBase CE02811 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68714 Major sperm protein protein 63 [Caenorhabditis elegans] ref|NP_495143.1| Major Sperm Protein family member (msp-63) [Caenorhabditis elegans] sp|Q21244|MSP63_CAEEL Major sperm protein 63 (MSP) 3.0E-71
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 1.0E-70
blast CAB03362 Hypothetical protein T13F2.11 [Caenorhabditis elegans] ref|NP_501742.1| Major Sperm Protein family member (msp-78) [Caenorhabditis elegans] sp|Q94053|MSP78_CAEEL Major sperm protein 78 (MSP) 2.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.4E-34
Sequence
Target Sequence 
>C. ELEGANS:172904_X_AT
tccgttccaccaggagacatccagacccaaccgggtacaaagatcgtcttcaatgctccg
tacgacgacaagcacacgtatcgcattaaagtgatcaactcttctgctcgccgtattggg
tacggtatcaagaccaccaacatgaagagacttggagttgatccaccatgtggagtgctg
gacccaaaggaagctgtacttctggcagtttcctgtgatgcattcgcatttggacaggaa
gacaccaacaacgaccgtatcaccgtcgaatggaccaacaccccggatggagctgccaag
cagttccgccgcgagtggttccagggagatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGTTCCACCAGGAGACATCCAGA 354 595 22 Antisense
CAAAGATCGTCTTCAATGCTCCGTA 197 193 59 Antisense
ATGCTCCGTACGACGACAAGCACAC 127 43 74 Antisense
TAAAGTGATCAACTCTTCTGCTCGC 542 641 108 Antisense
GGGTACGGTATCAAGACCACCAACA 483 497 139 Antisense
CACCATGTGGAGTGCTGGACCCAAA 609 199 185 Antisense
GAAGCTGTACTTCTGGCAGTTTCCT 383 341 211 Antisense
GGCAGTTTCCTGTGATGCATTCGCA 505 535 225 Antisense
GACCGTATCACCGTCGAATGGACCA 85 377 274 Antisense
GGATGGAGCTGCCAAGCAGTTCCGC 405 515 306 Antisense
GCCGCGAGTGGTTCCAGGGAGATGG 636 285 329 Antisense
Sequence Source Affymetrix Proprietary Database