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[172901_x_at] heat shock protein HSP16-48 /// heat shock protein HSP16-48

Description T27E4.9 /REP_DB=WormBase Gene ID /WP=CE14251 /GEN=hsp-16.49 /GB=AAB04841.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein HSP16-48
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 54      4grp_highest_8_8 > 37      4grp_high_8_8 > 01      6grp_highest_nonorm > 38      6grp_total > 55      4grp_total > 07      6grp_cgc > 55      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7766
1 2 3 4 range
A 7639.9
(1.2)
P 		4185.5
(-3)
P 		6316.3
(-0.4)
P 		9137.9
(3)
P 		4952
B 8285.1
(2.6)
P 		2632.9
(-3)
P 		6001.9
(0.3)
P 		8736.5
(3)
P 		6104
C 6975.4
(0.2)
P 		2984.2
(-3)
P 		5681.7
(-0.8)
P 		10399.2
(3)
P 		7415
avr 7633.5
(1.3) 		3267.5
(-3) 		6000
(-0.3) 		9424.5
(3) 		6157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4852
1 2 3 4 5 6 range
A 4655.7
(-3)
P 		6146.9
(-0.5)
P 		8207.6
(3)
P 		6146.3
(-0.5)
P 		5818.3
(-1)
P 		7318.1
(1.5)
P 		3552
B 5711.5
(-3)
P 		7895.5
(0.5)
P 		9508
(3)
P 		5986.7
(-2.6)
P 		6243.6
(-2.2)
P 		8293
(1.1)
P 		3797
C 5826.4
(-2.1)
P 		7717
(1.8)
P 		5381.7
(-3)
P 		6000
(-1.7)
P 		5693.6
(-2.4)
P 		8315.1
(3)
P 		2933
avr 5397.9
(-3) 		7253.1
(1.3) 		7699.1
(2.4) 		6044.3
(-1.5) 		5918.5
(-1.8) 		7975.4
(3) 		2578
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2131
1 2 3 4 range
A 2781.7
(3)
P 		908.6
(-3)
P 		1651.2
(-0.6)
P 		1337.6
(-1.6)
P 		1873
B 2642.1
(3)
P 		650.3
(-3)
P 		1390.8
(-0.8)
P 		1861.7
(0.6)
P 		1992
C 2309
(3)
P 		858
(-3)
P 		1980.3
(1.6)
P 		1753.5
(0.7)
P 		1451
avr 2577.6
(3) 		805.6
(-3) 		1674.1
(-0.1) 		1650.9
(-0.1) 		1772

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2234
1 2 3 4 5 6 range
A 858.2
(-3)
P 		2186.2
(3)
P 		1787.4
(1.2)
P 		1558.8
(0.2)
P 		1755.7
(1.1)
P 		1781.7
(1.2)
P 		1328
B 1987.3
(-1.4)
P 		2628.1
(1.2)
P 		2308.8
(-0.1)
P 		1585.7
(-3)
P 		2536.7
(0.8)
P 		3092.4
(3)
P 		1507
C 2159.6
(0.6)
P 		2354.7
(1.4)
P 		1369.7
(-2.4)
P 		1206.6
(-3)
P 		1842.4
(-0.6)
P 		2783.5
(3)
P 		1577
avr 1668.4
(-1.8) 		2389.7
(2.1) 		1822
(-1) 		1450.4
(-3) 		2044.9
(0.2) 		2552.5
(3) 		1102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172901_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12200
Sequence Type Exemplar sequence
Representative Public ID T27E4.9 NCBI
Target Description T27E4.9 /REP_DB=WormBase Gene ID /WP=CE14251 /GEN=hsp-16.49 /GB=AAB04841.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein HSP16-48
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072954(11), NM_072955(11), X01576(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072954 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-16.48) (hsp-16.48) mRNA, complete cds. 11/11 None
NM_072955 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-16.49) (hsp-16.49) mRNA, complete cds. 11/11 None
X01576 NCBI C. elegans mRNA 3' region for heat shock protein hsp 16-48. 10/11 None
SNAP00000044633 ENSEMBL cdna:SNAP chromosome:CEL140:V:9090718:9091204:1 11/11 None
SNAP00000044634 ENSEMBL cdna:SNAP chromosome:CEL140:V:9091867:9092353:-1 11/11 None
GENEFINDER00000044646 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9091867:9092353:-1 11/11 None
GENEFINDER00000044647 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9090718:9091204:1 11/11 None
T27E4.3 ENSEMBL cdna:known chromosome:CEL140:V:9090718:9091204:1 gene:T27E4.3 11/11 None
T27E4.9 ENSEMBL cdna:known chromosome:CEL140:V:9091867:9092353:-1 gene:T27E4.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182316 2/11 Cross Hyb Matching Probes None
X01577 1/11 Cross Hyb Matching Probes None
SNAP00000020848 2/11 Cross Hyb Matching Probes None
GENEFINDER00000020854 2/11 Cross Hyb Matching Probes None
Y46H3A.2 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9090718-9091205(+) 100.0 100.0
chrV:1805098-1805588(+) 92.13 92.13
chrV:9091867-9092354(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein HSP16-48
heat shock protein HSP16-48
Gene Symbol hsp-16.48
hsp-16.49
Ensembl T27E4.3
T27E4.9
Entrez Gene 179287 Entrez gene
179288 Entrez gene
SwissProt P02513 EMBL-EBI
WormBase CE14251 Wormbase
CE14251 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04841 Heat shock protein protein 16.49 [Caenorhabditis elegans] gb|AAB04840.1| Heat shock protein protein 16.48 [Caenorhabditis elegans] ref|NP_505356.1| Heat Shock Protein family member (hsp-16.49) [Caenorhabditis elegans] ref|NP_505355.1| Heat Shock Protein family member (hsp-16.48) [Caenorhabditis elegans] gb|AAA28069.1| heat shock protein 16-48 [Caenorhabditis elegans] sp|P02513|HSP17_CAEEL Heat shock protein Hsp16-48/Hsp16-49 6.0E-77
blast AAA28067 heat shock protein 16-48a [Caenorhabditis elegans] 2.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 4.1E-33
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 4.1E-33
Sequence
Target Sequence 
>C. ELEGANS:172901_X_AT
ccgttctccattttctgattcaaatgttctcgatcatttcttggatgaaatcactggatc
tgttcaatttccatattggagaaatgctgatcacaactcattcaatttttccgacaatat
tggagagattgtaaatgacgaatctaaattctctgttcaactcgatgtttctcatttcaa
accagaagatcttaaaattgaattggatggaagagaactaaaaattgaaggaattcaaga
aaaaaaatcagagcatggatactcgaaacgatcattttcaaaaatgattcttctaccaga
agatgttgatttaacttctgtcaaatctgcaatttcgaatgaaggaaaacttcaaattga
ggctccaaagaagactaactcatctcgttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTTCTCCATTTTCTGATTCAAAT 182 265 24 Antisense
AATGTTCTCGATCATTTCTTGGATG 155 165 46 Antisense
AAATCACTGGATCTGTTCAATTTCC 297 117 71 Antisense
GAGAAATGCTGATCACAACTCATTC 263 393 102 Antisense
CAACTCATTCAATTTTTCCGACAAT 427 187 117 Antisense
GAATCTAAATTCTCTGTTCAACTCG 128 327 163 Antisense
GTTCAACTCGATGTTTCTCATTTCA 242 445 178 Antisense
GAGCATGGATACTCGAAACGATCAT 204 387 274 Antisense
TTTCAAAAATGATTCTTCTACCAGA 402 669 299 Antisense
TTAACTTCTGTCAAATCTGCAATTT 380 683 334 Antisense
AAAGAAGACTAACTCATCTCGTTCT 573 123 390 Antisense
Sequence Source Affymetrix Proprietary Database