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[172900_x_at] Major sperm protein

Description ZK546.6 /REP_DB=WormBase Gene ID /WP=CE02920 /GEN=msp-152 /TR=Q23519 /GB=AAA68739.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster 6grp_total > 99      4grp_total > 01      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 41.3
(-2.2)
A 		15.1
(-3)
A 		69.2
(-1.4)
A 		224
(3)
P 		209
B 65.8
(-1.1)
A 		15
(-3)
A 		104
(0.3)
A 		174.6
(3)
A 		160
C 84.1
(-1.3)
A 		10.9
(-3)
A 		14.1
(-2.9)
A 		277
(3)
P 		266
avr 63.7
(-1.6) 		13.7
(-3) 		62.4
(-1.6) 		225.2
(3) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6443
1 2 3 4 5 6 range
A 21.2
(-3)
A 		38.8
(-3)
A 		117.9
(-2.9)
P 		627.9
(-2.4)
P 		4772.4
(1.5)
P 		6360.3
(3)
P 		6339
B 39
(-3)
A 		91.2
(-2.9)
A 		48.9
(-3)
A 		489.3
(-2.5)
P 		5605.3
(3)
P 		5403.1
(2.8)
P 		5566
C 27.2
(-3)
A 		216.9
(-2.8)
P 		66.9
(-3)
A 		536.2
(-2.5)
P 		6464.4
(3)
P 		6074.3
(2.6)
P 		6437
avr 29.1
(-3) 		115.6
(-2.9) 		77.9
(-3) 		551.1
(-2.5) 		5614
(2.7) 		5945.9
(3) 		5917
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 15.1
(-0.6)
A 		3.3
(-3)
A 		18.1
(0)
A 		32.8
(3)
P 		30
B 21
(0.1)
A 		3.7
(-3)
A 		24.1
(0.7)
A 		37.2
(3)
A 		34
C 27.8
(0.4)
A 		3.1
(-3)
A 		4.9
(-2.8)
A 		46.7
(3)
P 		44
avr 21.3
(0) 		3.4
(-3) 		15.7
(-0.9) 		38.9
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2274
1 2 3 4 5 6 range
A 3.9
(-3)
A 		13.8
(-3)
A 		25.7
(-2.9)
P 		159.3
(-2.4)
P 		1440.1
(2.6)
P 		1548.5
(3)
P 		1545
B 13.6
(-3)
A 		30.4
(-3)
A 		11.9
(-3)
A 		129.6
(-2.7)
P 		2277.4
(3)
P 		2014.8
(2.3)
P 		2266
C 10.1
(-3)
A 		66.2
(-2.8)
P 		17
(-3)
A 		107.8
(-2.7)
P 		2091.8
(3)
P 		2033.4
(2.8)
P 		2082
avr 9.2
(-3) 		36.8
(-2.9) 		18.2
(-3) 		132.2
(-2.6) 		1936.4
(3) 		1865.6
(2.8) 		1927
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172900_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5834
Sequence Type Exemplar sequence
Representative Public ID ZK546.6 NCBI
Target Description ZK546.6 /REP_DB=WormBase Gene ID /WP=CE02920 /GEN=msp-152 /TR=Q23519 /GB=AAA68739.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062500(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062500 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-152) (msp-152) mRNA, complete cds. 11/11 None
SNAP00000034943 ENSEMBL cdna:SNAP chromosome:CEL140:II:4933012:4933395:1 11/11 None
GENEFINDER00000034961 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4933012:4933395:1 11/11 None
ZK546.6 ENSEMBL cdna:known chromosome:CEL140:II:4933000:4933395:1 gene:ZK546.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062487 7/11 Cross Hyb Matching Probes A 
NM_062497 7/11 Cross Hyb Matching Probes A 
NM_069448 1/11 Cross Hyb Matching Probes None
NM_062558 2/11 Cross Hyb Matching Probes A 
NM_062559 1/11 Cross Hyb Matching Probes A 
NM_062571 1/11 Cross Hyb Matching Probes A 
NM_062505 3/11 Cross Hyb Matching Probes A 
NM_068359 2/11 Cross Hyb Matching Probes A 
NM_068377 1/11 Cross Hyb Matching Probes A 
NM_068379 1/11 Cross Hyb Matching Probes A 
NM_062490 4/11 Cross Hyb Matching Probes A 
NM_069321 2/11 Cross Hyb Matching Probes A 
NM_069339 1/11 Cross Hyb Matching Probes A 
NM_069341 1/11 Cross Hyb Matching Probes A 
NM_069358 1/11 Cross Hyb Matching Probes A 
NM_069359 2/11 Cross Hyb Matching Probes A 
NM_069361 1/11 Cross Hyb Matching Probes A 
NM_069380 1/11 Cross Hyb Matching Probes A 
NM_062457 2/11 Cross Hyb Matching Probes A 
NM_062464 2/11 Cross Hyb Matching Probes A 
SNAP00000000807 2/11 Cross Hyb Matching Probes None
SNAP00000000808 2/11 Cross Hyb Matching Probes None
GENEFINDER00000000821 2/11 Cross Hyb Matching Probes None
GENEFINDER00000000824 1/11 Cross Hyb Matching Probes None
SNAP00000007649 3/11 Cross Hyb Matching Probes None
GENEFINDER00000007652 3/11 Cross Hyb Matching Probes None
SNAP00000016470 2/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 2/11 Cross Hyb Matching Probes None
SNAP00000019951 2/11 Cross Hyb Matching Probes None
SNAP00000019952 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019958 2/11 Cross Hyb Matching Probes None
GENEFINDER00000019959 1/11 Cross Hyb Matching Probes None
SNAP00000021329 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021346 1/11 Cross Hyb Matching Probes None
SNAP00000034948 3/11 Cross Hyb Matching Probes None
GENEFINDER00000034965 3/11 Cross Hyb Matching Probes None
SNAP00000043868 7/11 Cross Hyb Matching Probes None
SNAP00000043869 4/11 Cross Hyb Matching Probes None
SNAP00000043876 7/11 Cross Hyb Matching Probes None
GENEFINDER00000043883 4/11 Cross Hyb Matching Probes None
GENEFINDER00000043888 7/11 Cross Hyb Matching Probes None
SNAP00000002639 2/11 Cross Hyb Matching Probes None
SNAP00000002641 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002661 2/11 Cross Hyb Matching Probes None
SNAP00000004243 1/11 Cross Hyb Matching Probes None
SNAP00000004244 2/11 Cross Hyb Matching Probes None
SNAP00000004246 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004263 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004265 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004273 1/11 Cross Hyb Matching Probes None
SNAP00000006013 1/11 Cross Hyb Matching Probes None
SNAP00000006015 1/11 Cross Hyb Matching Probes None
GENEFINDER00000006021 1/11 Cross Hyb Matching Probes None
GENEFINDER00000006024 1/11 Cross Hyb Matching Probes None
SNAP00000023386 2/11 Cross Hyb Matching Probes None
GENEFINDER00000023395 2/11 Cross Hyb Matching Probes None
SNAP00000040132 1/11 Cross Hyb Matching Probes None
SNAP00000041533 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041543 1/11 Cross Hyb Matching Probes None
SNAP00000043472 1/11 Cross Hyb Matching Probes None
SNAP00000043475 1/11 Cross Hyb Matching Probes None
GENEFINDER00000043484 1/11 Cross Hyb Matching Probes None
GENEFINDER00000043489 1/11 Cross Hyb Matching Probes None
F26G1.7 2/11 Cross Hyb Matching Probes A 
F26G1.8 2/11 Cross Hyb Matching Probes None
C33F10.9 2/11 Cross Hyb Matching Probes A 
R05F9.8 7/11 Cross Hyb Matching Probes A 
R05F9.3 4/11 Cross Hyb Matching Probes R A 
R05F9.13 7/11 Cross Hyb Matching Probes A 
ZK546.3 3/11 Cross Hyb Matching Probes A 
F59A6.8 3/11 Cross Hyb Matching Probes None
F58A6.8 2/11 Cross Hyb Matching Probes A 
F58A6.9 1/11 Cross Hyb Matching Probes A 
C34F11.4 1/11 Cross Hyb Matching Probes A 
F36H12.7 2/11 Cross Hyb Matching Probes A 
F36H12.6 2/11 Cross Hyb Matching Probes None
ZK354.11 1/11 Cross Hyb Matching Probes A 
ZK354.1 1/11 Cross Hyb Matching Probes A 
ZK1251.6 2/11 Cross Hyb Matching Probes A 
T13F2.10 1/11 Cross Hyb Matching Probes A 
T13F2.11 1/11 Cross Hyb Matching Probes A 
K07F5.1 1/11 Cross Hyb Matching Probes A 
K07F5.2 2/11 Cross Hyb Matching Probes A 
K07F5.3 1/11 Cross Hyb Matching Probes A 
F32B6.6 1/11 Cross Hyb Matching Probes A 
C04G2.3 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9894608-9894992(+) 94.01 94.01
chrIV:5316517-5316901(+) 93.75 93.75
chrIV:5297956-5298448(+) 93.75 93.75
chrIV:5062573-5062957(+) 92.71 92.71
chrIV:5260583-5261075(+) 93.23 93.23
chrII:4933009-4933393(+) 100.0 100.0
chrII:4894059-4899172(+) 96.88 96.88
chrII:4939885-4940265(+) 94.79 94.79
chrII:4825399-4825783(+) 94.27 94.27
chrII:5199774-5200158(+) 93.49 93.49
chrII:5194343-5194727(+) 92.97 92.97
chrIV:9766750-9767134(-) 94.27 94.27
chrIV:5312722-5313106(-) 93.23 93.23
chrIV:9836013-9836397(-) 93.23 93.23
chrIV:5064673-5065057(-) 92.97 92.97
chrIV:5294951-5295335(-) 92.97 92.97
chrII:4892294-4892678(-) 97.4 97.4
chrII:4916162-4916546(-) 97.4 97.4
chrII:5012855-5013235(-) 93.75 93.75
chrII:4785883-4786267(-) 93.75 93.75
Public Domain and Genome References
Gene Title Major sperm protein
Gene Symbol msp-152
Ensembl ZK546.6
Entrez Gene 191712 Entrez gene
SwissProt Q23519 EMBL-EBI
WormBase CE02920 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68739 Major sperm protein protein 152 [Caenorhabditis elegans] ref|NP_494901.1| Major Sperm Protein family member (msp-152) [Caenorhabditis elegans] sp|Q23519|MS152_CAEEL Major sperm protein 152 (MSP) 1.0E-71
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 4.0E-71
blast CAB03362 Hypothetical protein T13F2.11 [Caenorhabditis elegans] ref|NP_501742.1| Major Sperm Protein family member (msp-78) [Caenorhabditis elegans] sp|Q94053|MSP78_CAEEL Major sperm protein 78 (MSP) 6.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 4.7E-35
Sequence
Target Sequence 
>C. ELEGANS:172900_X_AT
gacccagccgggtacgaagatcgtcttcaatgcaccatacgatgacaagcacacctacca
catcaaggtgatcaactcatctgctcgccgtattggatacggtatcaagaccaccaatat
gaagagacttggagttgatccaccatgtggagttctggacccaaaggaagctgtgcttct
ggcagtttcctgcgatgccttcgcctttggacaggaggacactaacaacgatcgtatcac
tgttgagtggaccaacactccggatggtgctgctaagcaattccgtcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCCAGCCGGGTACGAAGATCGTC 583 381 45 Antisense
GAAGATCGTCTTCAATGCACCATAC 126 339 60 Antisense
CATACGATGACAAGCACACCTACCA 167 215 80 Antisense
CAAGGTGATCAACTCATCTGCTCGC 512 183 108 Antisense
TCATCTGCTCGCCGTATTGGATACG 186 621 121 Antisense
CCACCATGTGGAGTTCTGGACCCAA 388 271 184 Antisense
GGAAGCTGTGCTTCTGGCAGTTTCC 228 531 210 Antisense
ATGCCTTCGCCTTTGGACAGGAGGA 479 41 239 Antisense
GGAGGACACTAACAACGATCGTATC 217 513 258 Antisense
ACCAACACTCCGGATGGTGCTGCTA 294 85 295 Antisense
TGGTGCTGCTAAGCAATTCCGTCGG 316 553 309 Antisense
Sequence Source Affymetrix Proprietary Database