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[172889_x_at] major sperm protein

Description F58A6.8 /REP_DB=WormBase Gene ID /WP=CE02806 /GEN=msp-45 /TR=SW:P53017 /GB=AAA96204.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster 6grp_total > 98      4grp_total > 01      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 425
1 2 3 4 range
A 16
(-3)
A 		69.2
(-1.9)
A 		83.3
(-1.6)
A 		311.4
(3)
A 		295
B 114.4
(1.4)
A 		21.6
(-3)
A 		120.4
(1.6)
A 		149.6
(3)
P 		128
C 133.2
(-1.3)
A 		13.2
(-3)
A 		18.6
(-2.9)
A 		437.9
(3)
A 		425
avr 87.9
(-1.8) 		34.7
(-3) 		74.1
(-2.1) 		299.6
(3) 		265

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5273
1 2 3 4 5 6 range
A 71.5
(-3)
A 		39.3
(-3)
A 		111.3
(-2.9)
A 		562.8
(-2.4)
P 		3950.3
(1.5)
P 		5282.7
(3)
P 		5243
B 72.2
(-3)
A 		103.2
(-2.9)
A 		44.3
(-3)
A 		515.1
(-2.4)
P 		4273.5
(2.8)
P 		4447.6
(3)
P 		4403
C 10.2
(-3)
A 		207.1
(-2.8)
A 		49.9
(-3)
A 		540.2
(-2.4)
P 		4592
(2.3)
P 		5208.4
(3)
P 		5198
avr 51.3
(-3) 		116.5
(-2.9) 		68.5
(-3) 		539.4
(-2.4) 		4271.9
(2.1) 		4979.6
(3) 		4928
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 5.8
(-3)
A 		15
(-1.6)
A 		21.8
(-0.6)
A 		45.6
(3)
A 		40
B 36.5
(3)
A 		5.3
(-3)
A 		27.9
(1.3)
A 		31.9
(2.1)
P 		31
C 44.1
(0.5)
A 		3.8
(-3)
A 		6.5
(-2.8)
A 		73.8
(3)
A 		70
avr 28.8
(-0.1) 		8
(-3) 		18.7
(-1.5) 		50.4
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1740
1 2 3 4 5 6 range
A 13.2
(-3)
A 		14
(-3)
A 		24.2
(-2.9)
A 		142.7
(-2.4)
P 		1192
(2.6)
P 		1286.2
(3)
P 		1273
B 25.1
(-2.9)
A 		34.4
(-2.9)
A 		10.7
(-3)
A 		136.4
(-2.6)
P 		1736.3
(3)
P 		1658.5
(2.7)
P 		1726
C 3.8
(-3)
A 		63.2
(-2.8)
A 		12.7
(-3)
A 		108.6
(-2.6)
P 		1485.9
(2.1)
P 		1743.6
(3)
P 		1740
avr 14
(-3) 		37.2
(-2.9) 		15.9
(-3) 		129.2
(-2.6) 		1471.4
(2.6) 		1562.8
(3) 		1549
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172889_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5838
Sequence Type Exemplar sequence
Representative Public ID F58A6.8 NCBI
Target Description F58A6.8 /REP_DB=WormBase Gene ID /WP=CE02806 /GEN=msp-45 /TR=SW:P53017 /GB=AAA96204.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062558 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-45) (msp-45) mRNA, complete cds. 11/11 None
SNAP00000019951 ENSEMBL cdna:SNAP chromosome:CEL140:II:5156681:5157064:-1 11/11 None
GENEFINDER00000019958 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5155417:5157064:-1 11/11 None
F58A6.8 ENSEMBL cdna:known chromosome:CEL140:II:5156609:5157064:-1 gene:F58A6.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062497 4/11 Cross Hyb Matching Probes A 
NM_068370 2/11 Cross Hyb Matching Probes A 
NM_068310 2/11 Cross Hyb Matching Probes A 
NM_068312 2/11 Cross Hyb Matching Probes A 
NM_068313 3/11 Cross Hyb Matching Probes A 
NM_069063 1/11 Cross Hyb Matching Probes A 
NM_062559 5/11 Cross Hyb Matching Probes A 
NM_062569 1/11 Cross Hyb Matching Probes A 
NM_068351 1/11 Cross Hyb Matching Probes A 
NM_068354 1/11 Cross Hyb Matching Probes A 
NM_062505 1/11 Cross Hyb Matching Probes A 
NM_068359 2/11 Cross Hyb Matching Probes A 
NM_068377 2/11 Cross Hyb Matching Probes A 
NM_068379 2/11 Cross Hyb Matching Probes A 
NM_062490 3/11 Cross Hyb Matching Probes A 
NM_062500 2/11 Cross Hyb Matching Probes A 
NM_062743 1/11 Cross Hyb Matching Probes A 
NM_062748 1/11 Cross Hyb Matching Probes A 
NM_069321 1/11 Cross Hyb Matching Probes A 
NM_069359 1/11 Cross Hyb Matching Probes A 
NM_062457 1/11 Cross Hyb Matching Probes A 
NM_062464 2/11 Cross Hyb Matching Probes A 
NM_068372 3/11 Cross Hyb Matching Probes A 
SNAP00000000807 1/11 Cross Hyb Matching Probes None
SNAP00000000808 1/11 Cross Hyb Matching Probes None
GENEFINDER00000000821 1/11 Cross Hyb Matching Probes None
SNAP00000007649 3/11 Cross Hyb Matching Probes None
GENEFINDER00000007652 3/11 Cross Hyb Matching Probes None
SNAP00000016470 2/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 2/11 Cross Hyb Matching Probes None
SNAP00000019952 2/11 Cross Hyb Matching Probes None
GENEFINDER00000019959 5/11 Cross Hyb Matching Probes None
SNAP00000021327 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021348 1/11 Cross Hyb Matching Probes None
SNAP00000031245 1/11 Cross Hyb Matching Probes None
GENEFINDER00000031258 1/11 Cross Hyb Matching Probes None
SNAP00000034943 2/11 Cross Hyb Matching Probes None
SNAP00000034948 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034961 2/11 Cross Hyb Matching Probes None
GENEFINDER00000034965 1/11 Cross Hyb Matching Probes None
SNAP00000041369 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041398 1/11 Cross Hyb Matching Probes None
SNAP00000043869 3/11 Cross Hyb Matching Probes None
SNAP00000043876 4/11 Cross Hyb Matching Probes None
GENEFINDER00000043883 3/11 Cross Hyb Matching Probes None
SNAP00000002639 2/11 Cross Hyb Matching Probes None
SNAP00000002641 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002656 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002661 2/11 Cross Hyb Matching Probes None
SNAP00000004244 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004265 1/11 Cross Hyb Matching Probes None
SNAP00000006004 2/11 Cross Hyb Matching Probes None
SNAP00000006006 2/11 Cross Hyb Matching Probes None
SNAP00000006011 2/11 Cross Hyb Matching Probes None
SNAP00000006013 2/11 Cross Hyb Matching Probes None
SNAP00000006015 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006018 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006020 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006021 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006023 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006024 2/11 Cross Hyb Matching Probes None
SNAP00000007231 2/11 Cross Hyb Matching Probes None
GENEFINDER00000007236 2/11 Cross Hyb Matching Probes None
SNAP00000023386 1/11 Cross Hyb Matching Probes None
GENEFINDER00000023395 1/11 Cross Hyb Matching Probes None
SNAP00000029404 2/11 Cross Hyb Matching Probes None
SNAP00000029405 2/11 Cross Hyb Matching Probes None
GENEFINDER00000029413 2/11 Cross Hyb Matching Probes None
GENEFINDER00000029416 2/11 Cross Hyb Matching Probes None
SNAP00000029847 1/11 Cross Hyb Matching Probes None
GENEFINDER00000029849 1/11 Cross Hyb Matching Probes None
SNAP00000034129 1/11 Cross Hyb Matching Probes None
SNAP00000034134 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034137 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034140 1/11 Cross Hyb Matching Probes None
SNAP00000043994 2/11 Cross Hyb Matching Probes None
GENEFINDER00000044007 2/11 Cross Hyb Matching Probes None
F26G1.7 1/11 Cross Hyb Matching Probes A 
F26G1.8 1/11 Cross Hyb Matching Probes None
C33F10.9 2/11 Cross Hyb Matching Probes A 
R05F9.3 3/11 Cross Hyb Matching Probes R A 
R05F9.13 4/11 Cross Hyb Matching Probes A 
ZK546.6 2/11 Cross Hyb Matching Probes A 
ZK546.3 1/11 Cross Hyb Matching Probes A 
F59A6.8 4/11 Cross Hyb Matching Probes None
F58A6.9 2/11 Cross Hyb Matching Probes A 
C34F11.6 1/11 Cross Hyb Matching Probes A 
K05F1.2 1/11 Cross Hyb Matching Probes A 
ZK1248.6 1/11 Cross Hyb Matching Probes A 
C09B9.6 2/11 Cross Hyb Matching Probes A 
R13H9.2 2/11 Cross Hyb Matching Probes A 
R13H9.4 2/11 Cross Hyb Matching Probes A 
Y59E9AR.7 1/11 Cross Hyb Matching Probes A 
Y59E9AR.1 1/11 Cross Hyb Matching Probes A 
F36H12.7 2/11 Cross Hyb Matching Probes A 
ZK354.5 2/11 Cross Hyb Matching Probes A 
ZK354.4 2/11 Cross Hyb Matching Probes A 
ZK354.10 2/11 Cross Hyb Matching Probes None
ZK354.11 2/11 Cross Hyb Matching Probes A 
ZK354.1 2/11 Cross Hyb Matching Probes A 
Y59H11AM.1 1/11 Cross Hyb Matching Probes A 
ZK1251.6 1/11 Cross Hyb Matching Probes A 
K07F5.2 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4894059-4899171(+) 93.49 93.49
chrII:4825399-4825782(+) 92.45 92.45
chrII:4933009-4933392(+) 92.45 92.45
chrII:5194343-5194726(+) 91.93 91.93
chrII:5199774-5200157(+) 91.93 91.93
chrII:4939884-4940264(+) 91.41 91.41
chrIV:5316517-5316900(+) 93.75 93.75
chrIV:5062573-5062956(+) 93.23 93.23
chrIV:5297956-5298445(+) 93.23 93.23
chrIV:5260583-5260966(+) 91.67 91.67
chrIV:9770025-9770408(+) 91.67 91.67
chrIV:5249434-5249814(+) 91.15 91.15
chrII:5156678-5157062(-) 100.0 100.0
chrII:5012856-5013236(-) 93.49 93.49
chrII:4916163-4916546(-) 93.49 93.49
chrII:4892295-4892678(-) 91.93 91.93
chrIV:5294952-5295335(-) 93.23 93.23
chrIV:5055627-5056010(-) 92.45 92.45
chrIV:5064674-5065057(-) 92.19 92.19
chrIV:5312723-5313106(-) 92.19 92.19
Public Domain and Genome References
Gene Title major sperm protein
Gene Symbol msp-45
Ensembl F58A6.8
Entrez Gene 173884 Entrez gene
SwissProt P53017 EMBL-EBI
WormBase CE02806 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA96203 Serpentine receptor, class b (beta) protein 13 [Caenorhabditis elegans] ref|NP_494958.1| Serpentine Receptor, class B (beta) family member (srb-13) [Caenorhabditis elegans] 1.0E-141
blast CAE59055 Hypothetical protein CBG02338 [Caenorhabditis briggsae] 2.0E-92
blast CAE59056 Hypothetical protein CBG02339 [Caenorhabditis briggsae] 4.0E-89
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 1.0E-71
blast CAB03362 Hypothetical protein T13F2.11 [Caenorhabditis elegans] ref|NP_501742.1| Major Sperm Protein family member (msp-78) [Caenorhabditis elegans] sp|Q94053|MSP78_CAEEL Major sperm protein 78 (MSP) 2.0E-71
blast AAK68539 Hypothetical protein Y59H11AM.1 [Caenorhabditis elegans] ref|NP_501464.1| Y59H11AM.1 [Caenorhabditis elegans] 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 4.7E-35
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019958 5 140-159,179-201,225-247,279-301,316-338
Sequence
Target Sequence 
>C. ELEGANS:172889_X_AT
gacccagccgggtacgaagatcgtcttcaatgcaccatacgacgacaagcacacgtatca
catcaaagtgattaactcctcggctcgccgtattggatacggtatcaagaccaccaatat
gaagagacttggagttgatccaccatgtggagttttggacccaaaggaggcagttcttct
ggcagtttcctgtgatgcctttgccttcggtcaggaggatacaaacaacgaccgtatcac
cgttgagtggaccaataccccggatggagctgccaagcaattccgccgtgaatggttcca
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCCAGCCGGGTACGAAGATCGTC 585 381 45 Antisense
GAAGATCGTCTTCAATGCACCATAC 128 339 60 Antisense
GACAAGCACACGTATCACATCAAAG 461 365 88 Antisense
TCAAAGTGATTAACTCCTCGGCTCG 487 629 107 Antisense
ACTCCTCGGCTCGCCGTATTGGATA 102 99 119 Antisense
AAGGAGGCAGTTCTTCTGGCAGTTT 534 159 208 Antisense
GGCAGTTTCCTGTGATGCCTTTGCC 503 535 225 Antisense
CCTTTGCCTTCGGTCAGGAGGATAC 289 269 242 Antisense
CACCGTTGAGTGGACCAATACCCCG 326 199 282 Antisense
GGATGGAGCTGCCAAGCAATTCCGC 401 515 306 Antisense
GCAATTCCGCCGTGAATGGTTCCAA 210 317 321 Antisense
Sequence Source Affymetrix Proprietary Database