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[172883_x_at]

Description C27A2.2 /REP_DB=WormBase Gene ID /WP=CE04102 /GEN=rpl-22 /TR=SW:P52819 /GB=AAK31460.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=ribosomal protein L22
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 32      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 09      6grp_total > 31      4grp_total > 16      6grp_highest_sub > 02_03      6grp_cgc > 72      6grp_high1_sub > 02_03      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17536
1 2 3 4 range
A 26788.6
(-3)
P 		31694.2
(-0.8)
P 		33316.9
(-0.1)
P 		40085.6
(3)
P 		13297
B 28077.9
(-3)
P 		30337.5
(-1.2)
P 		31185.5
(-0.6)
P 		35696.2
(3)
P 		7618
C 24973.8
(-3)
P 		25445.6
(-2.8)
P 		27892.5
(-2)
P 		42510.2
(3)
P 		17536
avr 26613.4
(-3) 		29159.1
(-1.8) 		30798.3
(-1) 		39430.7
(3) 		12817

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7618
1 2 3 4 5 6 range
A 25285.7
(1.7)
P 		22950.6
(-0.6)
P 		26591.5
(3)
P 		25173.4
(1.6)
P 		20492.8
(-3)
P 		22387.7
(-1.1)
P 		6099
B 22310
(-0.3)
P 		26258.4
(3)
P 		25527
(2.4)
P 		22182.4
(-0.4)
P 		21118.5
(-1.2)
P 		18973.2
(-3)
P 		7285
C 22021.2
(-2.8)
P 		26444.8
(3)
P 		24547.7
(0.5)
P 		22146.1
(-2.7)
P 		22371.1
(-2.4)
P 		21891
(-3)
P 		4554
avr 23205.6
(-0.2) 		25217.9
(2.5) 		25555.4
(3) 		23167.3
(-0.2) 		21327.5
(-2.7) 		21084
(-3) 		4471
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3886
1 2 3 4 range
A 9753.7
(3)
P 		6879.9
(-1.4)
P 		8709.7
(1.4)
P 		5867.8
(-3)
P 		3886
B 8953.8
(3)
P 		7492.7
(-2.1)
P 		7226.4
(-3)
P 		7606.9
(-1.7)
P 		1727
C 8267
(-0.4)
P 		7315.8
(-2.7)
P 		9721.4
(3)
P 		7167.9
(-3)
P 		2554
avr 8991.5
(3) 		7229.5
(-2) 		8552.5
(1.8) 		6880.9
(-3) 		2111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4287
1 2 3 4 5 6 range
A 4661.2
(-3)
P 		8162.4
(3)
P 		5791.1
(-1.1)
P 		6384.6
(-0)
P 		6183.8
(-0.4)
P 		5450.7
(-1.6)
P 		3501
B 7762.8
(1)
P 		8740.3
(3)
P 		6198.7
(-2.3)
P 		5875.4
(-3)
P 		8580.2
(2.7)
P 		7075
(-0.5)
P 		2865
C 8162.3
(3)
P 		8069.1
(2.8)
P 		6247.8
(-0.1)
P 		4453.6
(-3)
P 		7239
(1.5)
P 		7328.2
(1.7)
P 		3709
avr 6862.1
(-0.2) 		8323.9
(3) 		6079.2
(-1.9) 		5571.2
(-3) 		7334.3
(0.8) 		6618
(-0.7) 		2753
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172883_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4628
Sequence Type Exemplar sequence
Representative Public ID C27A2.2 NCBI
Target Description C27A2.2 /REP_DB=WormBase Gene ID /WP=CE04102 /GEN=rpl-22 /TR=SW:P52819 /GB=AAK31460.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=ribosomal protein L22
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062531(11), NM_062532(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062531 NCBI Caenorhabditis elegans Ribosomal Protein, Large subunit family member (rpl-22) (rpl-22) mRNA, complete cds. 11/11 A 
NM_062532 NCBI Caenorhabditis elegans Ribosomal Protein, Large subunit family member (rpl-22) (rpl-22) mRNA, complete cds. 11/11 None
SNAP00000044048 ENSEMBL cdna:SNAP chromosome:CEL140:II:5055606:5056162:1 11/11 None
GENEFINDER00000044059 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5055606:5056162:1 11/11 None
C27A2.2a.2 ENSEMBL cdna:known chromosome:CEL140:II:5055523:5056205:1 gene:C27A2.2 11/11 None
C27A2.2b ENSEMBL cdna:known chromosome:CEL140:II:5055600:5056174:1 gene:C27A2.2 11/11 A 
C27A2.2a.1 ENSEMBL cdna:known chromosome:CEL140:II:5055600:5056205:1 gene:C27A2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5055603-5056160(+) 100.0 100.0
Public Domain and Genome References
Ensembl C27A2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494932 Ribosomal Protein, Large subunit family member (rpl-22) [Caenorhabditis elegans] gb|AAK31460.1| Ribosomal protein, large subunit protein 22, isoform a [Caenorhabditis elegans] sp|P52819|RL22_CAEEL 60S ribosomal protein L22 2.0E-36
blast CAE59029 Hypothetical protein CBG02309 [Caenorhabditis briggsae] 6.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR002671 EMBL-EBI Ribosomal L22e protein 3.4E-83
Pfam IPR002671 EMBL-EBI Ribosomal L22e protein 9.3E-14
Sequence
Target Sequence 
>C. ELEGANS:172883_X_AT
aagtcggccaagaaggctcttcgcaagaagaaggtgcacctcaagttcaacgttgagtgc
aagaacccagttgaggatggaattctcagaatcgaagatcttgaagccttcttgaacgag
aagatcaaggtcaatggaaagaccggacatttggccgctaacaacgtcaaggtcgaggtc
gccaagtccaaggtctcagtcgtctccgaggtcccattctcgaagagatacctcaagtac
cttaccaagaagtacctcaagagaaactccctccgtgattggctccgtgtggtcgctgtc
aacaagaacacctacgaggtcagatacttccacatcaacgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTCGGCCAAGAAGGCTCTTCGCA 196 155 34 Antisense
GAAGGTGCACCTCAAGTTCAACGTT 332 335 63 Antisense
GAAGATCTTGAAGCCTTCTTGAACG 45 339 127 Antisense
GAAAGACCGGACATTTGGCCGCTAA 660 351 170 Antisense
TGGCCGCTAACAACGTCAAGGTCGA 227 545 185 Antisense
ACGTCAAGGTCGAGGTCGCCAAGTC 10 87 197 Antisense
TCTCCGAGGTCCCATTCTCGAAGAG 312 611 236 Antisense
GAGATACCTCAAGTACCTTACCAAG 55 395 258 Antisense
CAAGAGAAACTCCCTCCGTGATTGG 350 185 291 Antisense
TCCGTGTGGTCGCTGTCAACAAGAA 405 591 317 Antisense
GGTCAGATACTTCCACATCAACGAC 675 501 351 Antisense
Sequence Source Affymetrix Proprietary Database