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[172881_x_at] histone H3

Description F17E9.10 /REP_DB=WormBase Gene ID /WP=CE03253 /GEN=his-32 /TR=Q9TW44 /GB=AAC05102.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=histone H3
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 88      6grp_total > 79      4grp_total > 25      6grp_highest_sub > Cu      6grp_cgc > 59      6grp_high1_sub > Cu      6grp_cgc_sub > Da      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1995
1 2 3 4 range
A 1330.6
(-3)
P 		1479.5
(-2.1)
P 		2054.2
(1.3)
P 		2341.2
(3)
P 		1011
B 1245.3
(-3)
P 		1777.8
(-1.4)
P 		1544.4
(-2.1)
P 		3239.9
(3)
P 		1995
C 1267.9
(-3)
P 		2076
(3)
P 		1722.7
(0.4)
P 		1846.1
(1.3)
P 		808
avr 1281.3
(-3) 		1777.8
(-0.5) 		1773.8
(-0.5) 		2475.7
(3) 		1194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 957
1 2 3 4 5 6 range
A 1215.9
(-2.2)
P 		1209.1
(-2.3)
P 		1205
(-2.3)
P 		1146.5
(-3)
P 		1420
(0.2)
P 		1662.3
(3)
P 		516
B 1369.7
(-0.4)
P 		1236
(-2)
P 		1153.4
(-3)
P 		1205.5
(-2.4)
P 		1456.3
(0.6)
P 		1657.8
(3)
P 		504
C 1271.3
(-1.4)
P 		1158.2
(-2.1)
P 		1144
(-2.2)
P 		1015
(-3)
P 		1609.2
(0.7)
P 		1972.2
(3)
P 		957
avr 1285.6
(-1.5) 		1201.1
(-2.3) 		1167.5
(-2.6) 		1122.3
(-3) 		1495.2
(0.5) 		1764.1
(3) 		642
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 379
1 2 3 4 range
A 484.5
(1.5)
P 		321.2
(-3)
P 		537
(3)
P 		342.7
(-2.4)
P 		216
B 397.1
(-2.3)
P 		439.1
(-1.5)
P 		357.9
(-3)
P 		690.4
(3)
P 		333
C 419.7
(-0.8)
P 		596.9
(2.9)
P 		600.4
(3)
P 		311.3
(-3)
P 		289
avr 433.8
(-3) 		452.4
(-1.3) 		498.4
(3) 		448.1
(-1.7) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 224.1
(-3)
P 		430
(3)
P 		262.4
(-1.9)
P 		290.8
(-1.1)
P 		428.5
(3)
P 		404.7
(2.3)
P 		206
B 476.6
(0.5)
P 		411.4
(-0.7)
P 		280.1
(-3)
P 		319.3
(-2.3)
P 		591.7
(2.5)
P 		618.2
(3)
P 		338
C 471.2
(0.5)
P 		353.4
(-1)
P 		291.2
(-1.9)
P 		204.1
(-3)
P 		520.7
(1.2)
P 		660.2
(3)
P 		456
avr 390.6
(-0.5) 		398.3
(-0.4) 		277.9
(-2.9) 		271.4
(-3) 		513.6
(2) 		561
(3) 		290
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172881_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9578
Sequence Type Exemplar sequence
Representative Public ID F17E9.10 NCBI
Target Description F17E9.10 /REP_DB=WormBase Gene ID /WP=CE03253 /GEN=his-32 /TR=Q9TW44 /GB=AAC05102.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=histone H3
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069006(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069006 NCBI Caenorhabditis elegans HIStone family member (his-32) (his-32) mRNA, complete cds. 11/11 None
SNAP00000000480 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8334287:8334697:1 11/11 None
GENEFINDER00000000500 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8334287:8334697:1 11/11 None
F17E9.10 ENSEMBL cdna:known chromosome:CEL140:IV:8334287:8334697:1 gene:F17E9.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069752 3/11 Cross Hyb Matching Probes A 
NM_068803 2/11 Cross Hyb Matching Probes A 
NM_064489 2/11 Cross Hyb Matching Probes A 
NM_072896 1/11 Cross Hyb Matching Probes A B 
NM_064493 2/11 Cross Hyb Matching Probes A 
NM_064494 2/11 Cross Hyb Matching Probes A 
NM_072875 1/11 Cross Hyb Matching Probes A B 
NM_074632 2/11 Cross Hyb Matching Probes A B 
NM_069733 3/11 Cross Hyb Matching Probes A 
NM_064498 1/11 Cross Hyb Matching Probes A 
NM_072891 1/11 Cross Hyb Matching Probes A B 
NM_069737 3/11 Cross Hyb Matching Probes A 
SNAP00000009815 2/11 Cross Hyb Matching Probes None
SNAP00000009819 2/11 Cross Hyb Matching Probes None
SNAP00000009820 2/11 Cross Hyb Matching Probes None
GENEFINDER00000009834 2/11 Cross Hyb Matching Probes None
GENEFINDER00000009837 2/11 Cross Hyb Matching Probes None
GENEFINDER00000009841 2/11 Cross Hyb Matching Probes None
SNAP00000034714 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034724 1/11 Cross Hyb Matching Probes None
SNAP00000002621 3/11 Cross Hyb Matching Probes None
GENEFINDER00000002632 3/11 Cross Hyb Matching Probes None
SNAP00000005174 2/11 Cross Hyb Matching Probes None
GENEFINDER00000005184 2/11 Cross Hyb Matching Probes None
SNAP00000028586 3/11 Cross Hyb Matching Probes None
GENEFINDER00000028598 3/11 Cross Hyb Matching Probes None
SNAP00000035840 3/11 Cross Hyb Matching Probes None
GENEFINDER00000035856 3/11 Cross Hyb Matching Probes None
SNAP00000015092 1/11 Cross Hyb Matching Probes None
GENEFINDER00000015146 1/11 Cross Hyb Matching Probes None
SNAP00000018183 1/11 Cross Hyb Matching Probes None
SNAP00000018188 1/11 Cross Hyb Matching Probes None
GENEFINDER00000018196 1/11 Cross Hyb Matching Probes None
GENEFINDER00000018198 1/11 Cross Hyb Matching Probes None
SNAP00000027916 2/11 Cross Hyb Matching Probes None
GENEFINDER00000027930 2/11 Cross Hyb Matching Probes None
ZK131.7 2/11 Cross Hyb Matching Probes A 
ZK131.3 2/11 Cross Hyb Matching Probes A 
ZK131.2 2/11 Cross Hyb Matching Probes A 
F08G2.3 1/11 Cross Hyb Matching Probes A 
F55G1.2 2/11 Cross Hyb Matching Probes A 
B0035.10 3/11 Cross Hyb Matching Probes A 
F54E12.1 3/11 Cross Hyb Matching Probes A 
F22B3.2 3/11 Cross Hyb Matching Probes A 
F07B7.5 1/11 Cross Hyb Matching Probes A B 
K06C4.13 1/11 Cross Hyb Matching Probes A B 
K06C4.5 1/11 Cross Hyb Matching Probes A B 
T10C6.13 2/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8334292-8334703(+) 100.0 100.0
chrIV:7488238-7488649(+) 94.16 94.16
chrIV:11326564-11326975(+) 93.92 93.92
chrII:13823541-13823952(+) 91.73 91.73
chrII:13820103-13820514(+) 91.73 91.73
chrV:8892625-8893036(+) 92.94 92.94
chrV:8850530-8850941(+) 92.94 92.94
chrX:7310054-7310464(+) 93.19 93.19
chrIV:11337829-11338240(-) 93.92 93.92
chrIV:11406427-11406838(-) 93.67 93.67
chrII:13828180-13828591(-) 91.97 91.97
chrII:13824742-13825153(-) 91.73 91.73
chrV:8900169-8900580(-) 92.7 92.7
chrV:16044455-16044866(-) 92.46 92.46
chrV:8537155-8537566(-) 92.46 92.46
Public Domain and Genome References
Gene Title histone H3
Gene Symbol his-32
Ensembl F17E9.10
Entrez Gene 177628 Entrez gene
SwissProt P08898 EMBL-EBI
WormBase CE03253 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264262_AT histone H3 at
ATGENOME1:17409_AT histone H3 at
CANINE_2:CFAAFFX.16507.1.S1_AT similar to CG31613-PA cfa
CANINE_2:CFAAFFX.945.1.S1_AT similar to CG31613-PA cfa
CANINE_2:CFAAFFX.945.1.S1_S_AT similar to CG31613-PA cfa
MOE430A:1460314_S_AT histone 2, H3c2 mm
MOUSE430_2:1460314_S_AT histone 2, H3c2 mm
MOUSE430A_2:1460314_S_AT histone 2, H3c2 mm
MG-U74AV2:94756_AT histone 2, H3c2 mm
MOUSE430_2:1422155_AT histone 2, H3c2 mm
MOE430A:1422155_AT histone 2, H3c2 mm
MOUSE430A_2:1422155_AT histone 2, H3c2 mm
MU11KSUBA:M32461_S_AT histone 2, H3c2 mm
MU11KSUBB:MSA.2871.0_AT histone 2, H2be mm
MG-U74AV2:102558_AT histone 2, H2be mm
MG-U74BV2:163827_AT histone 2, H2be mm
MOE430B:1455095_AT histone 2, H2be mm
MOUSE430_2:1455095_AT histone 2, H2be mm
MOE430B:1447854_S_AT histone 2, H2be mm
MOUSE430_2:1447854_S_AT histone 2, H2be mm
MU19KSUBA:TC25367_AT histone 2, H2be mm
RAE230B:1384367_AT histone 2, H3c2 (predicted) rn
RAT230_2:1384367_AT histone 2, H3c2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05834 Hypothetical protein ZK131.7 [Caenorhabditis elegans] emb|CAB05833.1| Hypothetical protein ZK131.3 [Caenorhabditis elegans] emb|CAB05831.1| Hypothetical protein ZK131.2 [Caenorhabditis elegans] emb|CAA97411.1| Hypothetical protein B0035.10 [Caenorhabditis elegans] emb|CAA92733.1| Hypothetical protein F22B3.2 [Caenorhabditis elegans] emb|CAB07653.1| Hypothetical protein T10C6.13 [Caenorhabditis elegans] emb|CAB05209.1| Hypothetical protein F54E12.1 [Caenorhabditis elegans] emb|CAB04057.1| Hypothetical protein F08G2.3 [Caenorhabditis elegans] gb|AAC05102.1| Histone protein 32 [Caenorhabditis elegans] gb|AAK84514.1| Histone protein 49 [Caenorhabditis elegans] gb|AAF98226.1| Histone protein 17 [Caenorhabditis elegans] gb|AAF98231.1| Histone protein 27 [Caenorhabditis elegans] emb|CAA33644.1| Histone protein [Caenorhabditis elegans] gb|AAB00650.1| Histone protein 59 [Caenorhabditis elegans] gb|AAC48033.1| Histone protein 6 [Caenorhabditis elegans] ref|NP_505292.1| HIStone family member (his-27) [Caenorhabditis elegans] ref|NP_505297.1| HIStone family member (his-17) [Caenorhabditis elegans] ref|NP_496890.1| HIStone family member (his-13) [Caenorhabditis elegans] ref|NP_505199.1| HIStone family member (his-6) [Caenorhabditis elegans] ref|NP_501204.1| F55G1.2 [Caenorhabditis elegans] ref|NP_502138.1| HIStone family member (his-55) [Caenorhabditis elegans] ref|NP_502153.1| HIStone family member (his-63) [Caenorhabditis elegans] ref|NP_496899.1| HIStone family member (his-42) [Caenorhabditis elegans] ref|NP_505276.1| HIStone family member (his-49) [Caenorhabditis elegans] ref|NP_502134.1| HIStone family member (his-45) [Caenorhabditis elegans] ref|NP_507033.1| HIStone family member (his-2) [Caenorhabditis elegans] ref|NP_501407.1| HIStone family member (his-32) [Caenorhabditis elegans] ref|NP_496895.1| HIStone family member (his-25) [Caenorhabditis elegans] ref|NP_496894.1| HIStone family member (his-9) [Caenorhabditis elegans] gb|AAG50235.1| histone H3 [Caenorhabditis elegans] pir||HSKW3 histone H3 - Caenorhabditis elegans 5.0E-70
blast P08898 Histone H3 1.0E-69
blast AAH74969 H3/o protein [Homo sapiens] 2.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007125 EMBL-EBI Histone core 1.6E-29
Sequence
Target Sequence 
>C. ELEGANS:172881_X_AT
aaagcaattggccaccaaggctgcccgcaagtcggccccagcttcaggaggagtcaagaa
accacatcgttaccgtccaggaaccgtcgctcttcgtgagatcagacgttaccagaagtc
caccgagcttctcatccgcagagcgccattccagcgcctcgttcgtgagatcgctcagga
tttcaagaccgatcttcgtttccagtcttccgccgtcatggctcttcaagaggctgccga
ggcttacctcgtcggactcttcgaggacaccaacttgtgcgccattcacgctaagcgagt
tac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCAATTGGCCACCAAGGCTGCC 545 121 66 Antisense
TCAAGAAACCACATCGTTACCGTCC 274 627 119 Antisense
GTCGCTCTTCGTGAGATCAGACGTT 420 475 151 Antisense
AGACGTTACCAGAAGTCCACCGAGC 146 69 169 Antisense
GAGATCGCTCAGGATTTCAAGACCG 231 395 232 Antisense
AAGACCGATCTTCGTTTCCAGTCTT 706 153 250 Antisense
CGCCGTCATGGCTCTTCAAGAGGCT 482 255 276 Antisense
AAGAGGCTGCCGAGGCTTACCTCGT 193 155 293 Antisense
TTACCTCGTCGGACTCTTCGAGGAC 549 681 309 Antisense
TTCGAGGACACCAACTTGTGCGCCA 674 695 325 Antisense
GCGCCATTCACGCTAAGCGAGTTAC 324 287 344 Antisense
Sequence Source Affymetrix Proprietary Database