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[172873_x_at]

Description F35G12.3B /REP_DB=WormBase Gene ID /WP=CE00972 /TR=Q20056 /GB=CAA86332.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 91      6grp_total > 69      4grp_total > 38      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 333.2
(3)
P 		224.2
(-2.3)
P 		223.3
(-2.4)
P 		210.9
(-3)
P 		122
B 336.7
(3)
P 		212.3
(-3)
P 		246.1
(-1.4)
P 		297.3
(1.1)
P 		124
C 385.3
(3)
P 		360.8
(2)
P 		233.7
(-3)
P 		331
(0.9)
P 		152
avr 351.7
(3) 		265.8
(-1.4) 		234.4
(-3) 		279.7
(-0.7) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 280.2
(0.1)
P 		227.1
(-1.3)
P 		174.9
(-2.8)
P 		168.2
(-3)
P 		241.4
(-0.9)
P 		382.1
(3)
P 		214
B 185.9
(-3)
P 		202.3
(-2.3)
P 		185
(-3)
P 		240.2
(-0.7)
P 		244.7
(-0.5)
P 		328
(3)
P 		143
C 258.4
(0.1)
P 		209.5
(-1.3)
P 		249.3
(-0.2)
P 		149.5
(-3)
P 		282.5
(0.7)
P 		362.7
(3)
P 		213
avr 241.5
(-1.1) 		213
(-2.1) 		203.1
(-2.4) 		186
(-3) 		256.2
(-0.5) 		357.6
(3) 		172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 121.3
(3)
P 		48.7
(-1.8)
P 		58.4
(-1.2)
P 		30.9
(-3)
P 		90
B 107.4
(3)
P 		52.4
(-3)
P 		57
(-2.5)
P 		63.4
(-1.8)
P 		55
C 127.5
(3)
P 		103.7
(1)
P 		81.4
(-0.9)
P 		55.8
(-3)
P 		72
avr 118.7
(3) 		68.3
(-1.4) 		65.6
(-1.6) 		50
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 51.7
(-1.5)
P 		80.8
(1.7)
P 		38.1
(-3)
P 		42.7
(-2.5)
P 		72.8
(0.8)
P 		93
(3)
P 		55
B 64.7
(-1.5)
P 		67.3
(-1.3)
P 		44.9
(-3)
P 		63.6
(-1.6)
P 		99.4
(1.2)
P 		122.3
(3)
P 		77
C 95.8
(1.3)
P 		63.9
(-0.8)
P 		63.5
(-0.8)
P 		30.1
(-3)
P 		91.4
(1)
P 		121.4
(3)
P 		91
avr 70.7
(-0.7) 		70.7
(-0.7) 		48.8
(-2.7) 		45.5
(-3) 		87.9
(0.8) 		112.2
(3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172873_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7066
Sequence Type Exemplar sequence
Representative Public ID F35G12.3B NCBI
Target Description F35G12.3B /REP_DB=WormBase Gene ID /WP=CE00972 /TR=Q20056 /GB=CAA86332.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027391(9), NM_001027392(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027391 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-5) (sel-5) mRNA, complete cds. 9/11 None
NM_001027392 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-5) (sel-5) mRNA, complete cds. 11/11 None
SNAP00000042237 ENSEMBL cdna:SNAP chromosome:CEL140:III:4572081:4578392:-1 9/11 None
GENEFINDER00000042263 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4572081:4578392:-1 9/11 None
F35G12.3b ENSEMBL cdna:known chromosome:CEL140:III:4571756:4578402:-1 gene:F35G12.3 11/11 A 
F35G12.3a ENSEMBL cdna:known chromosome:CEL140:III:4571780:4578392:-1 gene:F35G12.3 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4575160-4578392(-) 100.0 100.0
Public Domain and Genome References
Ensembl F35G12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86326 Hypothetical protein F35G12.3a [Caenorhabditis elegans] ref|NP_001022562.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71545.1| SEL-5A serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAE71182 Hypothetical protein CBG18039 [Caenorhabditis briggsae] 0.0
blast CAA86332 Hypothetical protein F35G12.3b [Caenorhabditis elegans] ref|NP_001022563.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71546.1| SEL-5B serine/threonine kinase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.9E-36
Sequence
Target Sequence 
>C. ELEGANS:172873_X_AT
gatctggataaacctgctcttccgagagatcgtgctcaaactgatggaaaacgaagactt
cctcacgaatcggacattatttttcaacagcagcatcggagaaatgtcagcgacactagc
caaatttcaaggagcgctttcaagccgtacagtagtcaacaaactacgtcaaaaactagt
tcacaagttgttcgaagcgtcgaggacatgtcacaacggcagaatggtggatcgggagaa
tggaatccattcctggttgcaccgttttcaaataactcgatcagtcggaaagatggacag
gagagtgcatttatgatggatgattcgcattttggaatggtttttgatgaaattcggaga
aaagaaattcccgccgaattggacagtgaaacatcctcaattgattctcgtgatccattc
ggagctgctccatttgatcaattaacagtatcaacatcatcatctgcacaaccagtatct
ctaccaccaggtagtgttcatcttcacacctcaatagcaccttccccattattacggcac
gtactaacgaaagctcctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTGGATAAACCTGCTCTTCCGA 98 419 1483 Antisense
GCTCTTCCGAGAGATCGTGCTCAAA 342 301 1498 Antisense
GAAGACTTCCTCACGAATCGGACAT 72 339 1535 Antisense
AGGAGCGCTTTCAAGCCGTACAGTA 509 53 1612 Antisense
ATTCCTGGTTGCACCGTTTTCAAAT 339 1 1731 Antisense
GATTCTCGTGATCCATTCGGAGCTG 78 433 1885 Antisense
GGAGCTGCTCCATTTGATCAATTAA 438 521 1903 Antisense
GTATCAACATCATCATCTGCACAAC 546 453 1930 Antisense
TCTGCACAACCAGTATCTCTACCAC 415 609 1945 Antisense
AGGTAGTGTTCATCTTCACACCTCA 479 57 1971 Antisense
GGCACGTACTAACGAAAGCTCCTCC 40 535 2018 Antisense
Sequence Source Affymetrix Proprietary Database