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[172865_x_at] 60S ribosomal protein L27

Description C53H9.1 /REP_DB=WormBase Gene ID /WP=CE19381 /GEN=rpl-27 /GB=AAK68266.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=60S ribosomal protein L27
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 19      6grp_total > 41      4grp_total > 17      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17462
1 2 3 4 range
A 22262.4
(-3)
P 		29542.3
(-0.3)
P 		26751.3
(-1.4)
P 		38737.8
(3)
P 		16475
B 22056.9
(-3)
P 		24629.9
(-1.2)
P 		24941.3
(-1)
P 		30614.7
(3)
P 		8558
C 21275.8
(-3)
P 		22298.6
(-2.6)
P 		22291.1
(-2.6)
P 		36600
(3)
P 		15324
avr 21865
(-3) 		25490.3
(-1.4) 		24661.2
(-1.8) 		35317.5
(3) 		13453

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6653
1 2 3 4 5 6 range
A 20318.1
(0.2)
P 		20446
(0.3)
P 		23372.6
(3)
P 		22829.9
(2.5)
P 		16784.3
(-3)
P 		19937.8
(-0.1)
P 		6588
B 20367.9
(1.1)
P 		20771.9
(1.5)
P 		22115.4
(3)
P 		18682.7
(-0.8)
P 		17368.3
(-2.3)
P 		16720.1
(-3)
P 		5395
C 20469.5
(-0.1)
P 		22843.8
(2.7)
P 		23132.1
(3)
P 		22571.5
(2.3)
P 		19926.8
(-0.7)
P 		18002.4
(-3)
P 		5130
avr 20385.2
(-0.1) 		21353.9
(1.1) 		22873.4
(3) 		21361.4
(1.1) 		18026.5
(-3) 		18220.1
(-2.8) 		4847
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2435
1 2 3 4 range
A 8105.7
(3)
P 		6412.8
(-1.2)
P 		6993.3
(0.3)
P 		5670.5
(-3)
P 		2435
B 7033.8
(3)
P 		6083
(-1.5)
P 		5779.5
(-3)
P 		6524
(0.6)
P 		1254
C 7042.9
(0.3)
P 		6411
(-2.1)
P 		7769.1
(3)
P 		6171.3
(-3)
P 		1598
avr 7394.1
(3) 		6302.3
(-2.1) 		6847.3
(0.4) 		6121.9
(-3) 		1272

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3842
1 2 3 4 5 6 range
A 3745.5
(-3)
P 		7271.6
(3)
P 		5090.1
(-0.7)
P 		5790.2
(0.5)
P 		5064.8
(-0.8)
P 		4854.2
(-1.1)
P 		3526
B 7087
(3)
P 		6914.1
(2.5)
P 		5370.3
(-1.8)
P 		4948.4
(-3)
P 		7056.5
(2.9)
P 		6234.9
(0.6)
P 		2139
C 7587.1
(3)
P 		6970.3
(1.8)
P 		5887.5
(-0.3)
P 		4539.2
(-3)
P 		6448
(0.8)
P 		6026.4
(-0.1)
P 		3048
avr 6139.9
(0.2) 		7052
(3) 		5449.3
(-1.9) 		5092.6
(-3) 		6189.8
(0.4) 		5705.2
(-1.1) 		1959
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172865_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.305
Sequence Type Exemplar sequence
Representative Public ID C53H9.1 NCBI
Target Description C53H9.1 /REP_DB=WormBase Gene ID /WP=CE19381 /GEN=rpl-27 /GB=AAK68266.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=60S ribosomal protein L27
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058504(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058504 NCBI Caenorhabditis elegans Ribosomal Protein, Large subunit family member (rpl-27) (rpl-27) mRNA, complete cds. 11/11 None
SNAP00000007150 ENSEMBL cdna:SNAP chromosome:CEL140:I:1834890:1835395:1 11/11 None
GENEFINDER00000007154 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1834890:1835395:1 11/11 None
C53H9.1.2 ENSEMBL cdna:known chromosome:CEL140:I:1834882:1835440:1 gene:C53H9.1 11/11 None
C53H9.1.1 ENSEMBL cdna:known chromosome:CEL140:I:1834884:1835440:1 gene:C53H9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1834889-1835395(+) 100.0 100.0
Public Domain and Genome References
Gene Title 60S ribosomal protein L27
Gene Symbol rpl-27
Ensembl C53H9.1
Entrez Gene 171750 Entrez gene
SwissProt P91914 EMBL-EBI
WormBase CE19381 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:19123_AT 60S ribosomal protein L27 (RPL27A) at
ATH1-121501:265730_AT 60S ribosomal protein L27 (RPL27A) at
CANINE:1604140_S_AT ribosomal protein L27 cfa
CANINE:1601199_AT ribosomal protein L27 cfa
CANINE_2:CFAAFFX.16942.1.S1_AT ribosomal protein L27 cfa
CANINE_2:CFAAFFX.22540.1.S1_S_AT ribosomal protein L27 cfa
CANINE:1601200_AT Ribosomal protein L27 cfa
DROSOPHILA_2:1632335_AT Ribosomal protein L27 dm
DROSGENOME1:150604_AT Ribosomal protein L27 dm
CHICKEN:GGA.743.1.S1_S_AT ribosomal protein L27 gga
CHICKEN:GGAAFFX.20503.1.S1_S_AT ribosomal protein L27 gga
PLASMODIUM_ANOPHELES:200025_S_AT ribosomal protein L27 hs
HG-U133_PLUS_2:200025_S_AT ribosomal protein L27 hs
HG-FOCUS:200025_S_AT ribosomal protein L27 hs
HG-U133A_2:200025_S_AT ribosomal protein L27 hs
HG-U133A:200025_S_AT ribosomal protein L27 hs
HG-U133B:200025_S_AT ribosomal protein L27 hs
U133_X3P:200025_S_AT ribosomal protein L27 hs
HUGENEFL:L19527_AT ribosomal protein L27 hs
U133_X3P:G4506622_3P_A_AT ribosomal protein L27 hs
HG-U95AV2:39830_AT ribosomal protein L27 hs
MOE430A:1453096_X_AT ribosomal protein L27 mm
MOUSE430A_2:1453096_X_AT ribosomal protein L27 mm
MOUSE430_2:1453096_X_AT ribosomal protein L27 mm
MOE430A:1416099_AT ribosomal protein L27 mm
MOUSE430A_2:1416099_AT ribosomal protein L27 mm
MOUSE430_2:1416099_AT ribosomal protein L27 mm
MOE430A:1448217_A_AT ribosomal protein L27 mm
MOUSE430A_2:1448217_A_AT ribosomal protein L27 mm
MOUSE430_2:1448217_A_AT ribosomal protein L27 mm
MU11KSUBA:AA518088_AT Ribosomal protein L27, mRNA (cDNA clone MGC:35949 IMAGE:4986323) mm
RG-U34A:RC_AI176589_AT ribosomal protein L27 rn
RG-U34A:RC_AI176589_G_AT ribosomal protein L27 rn
RG-U34C:RC_AI101165_AT ribosomal protein L27 rn
RAE230A:1367561_AT ribosomal protein L27 rn
RAT230_2:1367561_AT ribosomal protein L27 rn
YEAST_2:1777555_AT Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similarity to rat L27 ribosomal protein Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68266 Ribosomal protein, large subunit protein 27 [Caenorhabditis elegans] ref|NP_490905.1| Ribosomal Protein, Large subunit family member (rpl-27) [Caenorhabditis elegans] sp|P91914|RL27_CAEEL 60S ribosomal protein L27 gb|AAB48626.1| ribosomal protein L27 homolog [Caenorhabditis elegans] 1.0E-72
blast CAE74466 Hypothetical protein CBG22212 [Caenorhabditis briggsae] 8.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR005824 EMBL-EBI KOW 3.0E-6
Pfam IPR001141 EMBL-EBI Ribosomal protein L27e 2.7E-68
Sequence
Target Sequence 
>C. ELEGANS:172865_X_AT
tgaagcctggaaaggtcgtcctcgtcctcagaggaaagtatgctggtcgcaaggccgtcg
tcgtcaagcaacaagacgagggagtttcggacagaacctacccacacgccatcattgctg
gaatcgacagatacccattgaaggttaccaaggacatgggaaagaagaagatcgagaaga
gaaataagctgaagccattcctcaaggtcgtctcctacactcacttgctcccaactcgtt
actccgtcgatgtcgccttcgataagaccaacatcaacaaggaggcactcaaggctccgt
ccaagaagagaaaggccctcgtcgaagtcaagtccaagttcgaggagcgttacaagaccg
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGCCTGGAAAGGTCGTCCTCGT 298 573 26 Antisense
GTCGTCCTCGTCCTCAGAGGAAAGT 704 465 40 Antisense
AAAGTATGCTGGTCGCAAGGCCGTC 546 119 60 Antisense
GCCGTCGTCGTCAAGCAACAAGACG 513 283 79 Antisense
CCACACGCCATCATTGCTGGAATCG 479 275 127 Antisense
GCTGGAATCGACAGATACCCATTGA 319 295 142 Antisense
TAAGCTGAAGCCATTCCTCAAGGTC 141 637 210 Antisense
GATGTCGCCTTCGATAAGACCAACA 410 407 274 Antisense
CAAGGAGGCACTCAAGGCTCCGTCC 6 185 303 Antisense
AGAAAGGCCCTCGTCGAAGTCAAGT 508 71 334 Antisense
GTTCGAGGAGCGTTACAAGACCGGA 178 443 363 Antisense
Sequence Source Affymetrix Proprietary Database