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[172833_x_at] histone H3

Description T10C6.13 /REP_DB=WormBase Gene ID /WP=CE03253 /GEN=his-2 /TR=Q9TW44 /GB=CAB07653.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=histone H3
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 04      4grp_high_8_8 > 38      6grp_highest_nonorm > 78      6grp_total > 88      4grp_total > 41      6grp_highest_sub > Da      6grp_cgc > 59      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 554
1 2 3 4 range
A 1917.7
(-3)
P 		2472
(3)
P 		2436.8
(2.6)
P 		2315.9
(1.3)
P 		554
B 2023.1
(-3)
P 		2279.2
(1.6)
P 		2359.6
(3)
P 		2149.7
(-0.7)
P 		337
C 2053.4
(-3)
P 		2303.1
(3)
P 		2097
(-2)
P 		2263.7
(2.1)
P 		250
avr 1998.1
(-3) 		2351.4
(3) 		2297.8
(2.1) 		2243.1
(1.2) 		353

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 756
1 2 3 4 5 6 range
A 1271.8
(-3)
P 		1515
(-0.4)
P 		1409.4
(-1.5)
P 		1623.7
(0.8)
P 		1650.1
(1.1)
P 		1822.7
(3)
P 		551
B 1527.9
(-1)
P 		1391.4
(-2.2)
P 		1484.3
(-1.4)
P 		1295.7
(-3)
P 		1635.2
(-0.1)
P 		1993.3
(3)
P 		698
C 1477.3
(-2.1)
P 		1398.8
(-2.9)
P 		1382.9
(-3)
P 		1461.6
(-2.3)
P 		1766.2
(0.6)
P 		2028
(3)
P 		645
avr 1425.7
(-3) 		1435.1
(-2.9) 		1425.5
(-3) 		1460.3
(-2.6) 		1683.8
(-0) 		1948
(3) 		523
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 392
1 2 3 4 range
A 698.2
(3)
P 		536.6
(0.3)
P 		637
(2)
P 		339
(-3)
P 		359
B 645.1
(3)
P 		562.9
(0.4)
P 		546.8
(-0.2)
P 		458.1
(-3)
P 		187
C 679.7
(2.1)
P 		662.2
(1.8)
P 		730.9
(3)
P 		381.7
(-3)
P 		349
avr 674.3
(3) 		587.2
(1.1) 		638.2
(2.2) 		392.9
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 509
1 2 3 4 5 6 range
A 234.4
(-3)
P 		538.8
(3)
P 		306.9
(-1.6)
P 		411.8
(0.5)
P 		497.9
(2.2)
P 		443.8
(1.1)
P 		304
B 531.6
(-0.2)
P 		463.1
(-1.2)
P 		360.4
(-2.7)
P 		343.2
(-3)
P 		664.4
(1.8)
P 		743.3
(3)
P 		400
C 547.6
(1)
P 		426.8
(-0.9)
P 		352
(-2.1)
P 		293.9
(-3)
P 		571.5
(1.3)
P 		678.9
(3)
P 		385
avr 437.9
(-0.9) 		476.2
(-0.1) 		339.8
(-3) 		349.6
(-2.8) 		577.9
(2.1) 		622
(3) 		282
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172833_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13427
Sequence Type Exemplar sequence
Representative Public ID T10C6.13 NCBI
Target Description T10C6.13 /REP_DB=WormBase Gene ID /WP=CE03253 /GEN=his-2 /TR=Q9TW44 /GB=CAB07653.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=histone H3
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074632(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074632 NCBI Caenorhabditis elegans HIStone family member (his-2) (his-2) mRNA, complete cds. 11/11 None
SNAP00000027916 ENSEMBL cdna:SNAP chromosome:CEL140:V:16044456:16044866:-1 11/11 None
GENEFINDER00000027930 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16044456:16044866:-1 11/11 None
T10C6.13 ENSEMBL cdna:known chromosome:CEL140:V:16044152:16044866:-1 gene:T10C6.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068803 2/11 Cross Hyb Matching Probes A 
NM_067207 1/11 Cross Hyb Matching Probes A B 
NM_072798 3/11 Cross Hyb Matching Probes A B 
NM_072896 3/11 Cross Hyb Matching Probes A B 
NM_072875 3/11 Cross Hyb Matching Probes A B 
NM_069006 2/11 Cross Hyb Matching Probes A B 
NM_072891 3/11 Cross Hyb Matching Probes A B 
SNAP00000016068 1/11 Cross Hyb Matching Probes None
GENEFINDER00000016112 1/11 Cross Hyb Matching Probes None
SNAP00000000480 2/11 Cross Hyb Matching Probes None
GENEFINDER00000000500 2/11 Cross Hyb Matching Probes None
SNAP00000005174 2/11 Cross Hyb Matching Probes None
GENEFINDER00000005184 2/11 Cross Hyb Matching Probes None
SNAP00000015092 3/11 Cross Hyb Matching Probes None
GENEFINDER00000015146 3/11 Cross Hyb Matching Probes None
SNAP00000018183 3/11 Cross Hyb Matching Probes None
SNAP00000018188 3/11 Cross Hyb Matching Probes None
GENEFINDER00000018196 3/11 Cross Hyb Matching Probes None
GENEFINDER00000018198 3/11 Cross Hyb Matching Probes None
SNAP00000030773 3/11 Cross Hyb Matching Probes None
GENEFINDER00000030784 3/11 Cross Hyb Matching Probes None
GENEFINDER00000013875 3/11 Cross Hyb Matching Probes None
SNAP00000013868 4/11 Cross Hyb Matching Probes None
Y49E10.6.2 1/11 Cross Hyb Matching Probes None
Y49E10.6.1 1/11 Cross Hyb Matching Probes None
F55G1.2 2/11 Cross Hyb Matching Probes A 
F17E9.10 2/11 Cross Hyb Matching Probes A B 
F45F2.13 3/11 Cross Hyb Matching Probes A B 
F07B7.5 3/11 Cross Hyb Matching Probes A B 
K06C4.13 3/11 Cross Hyb Matching Probes A B 
K06C4.5 3/11 Cross Hyb Matching Probes A B 
K03A1.1 4/11 Cross Hyb Matching Probes B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13823541-13823952(+) 90.27 90.27
chrII:13820103-13820514(+) 90.27 90.27
chrV:8892625-8893036(+) 96.84 96.84
chrV:8850530-8850941(+) 96.84 96.84
chrX:7310054-7310464(+) 96.59 96.59
chrII:13824742-13825153(-) 90.27 90.27
chrV:16044455-16044866(-) 100.0 100.0
chrV:8900169-8900580(-) 96.59 96.59
chrV:8537155-8537566(-) 96.35 96.35
Public Domain and Genome References
Gene Title histone H3
Gene Symbol his-2
Ensembl T10C6.13
Entrez Gene 180074 Entrez gene
SwissProt P08898 EMBL-EBI
WormBase CE03253 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262979_S_AT histone H3, putative at
ATH1-121501:259387_AT histone H3, putative at
CANINE_2:CFAAFFX.14172.1.S1_AT similar to H3 histone, family 3B cfa
CANINE_2:CFAAFFX.2698.1.S1_S_AT similar to H3 histone, family 3B cfa
CANINE_2:CFAAFFX.357.1.S1_X_AT similar to H3 histone, family 3B cfa
HG-U133_PLUS_2:208575_AT histone 1, H3a hs
HG-U133A:208575_AT histone 1, H3a hs
HG-FOCUS:208575_AT histone 1, H3a hs
HG-U133A_2:208575_AT histone 1, H3a hs
HUGENEFL:Z46261_AT histone 1, H3a hs
U133_X3P:G4504280_3P_X_AT histone 1, H3a hs
U133_X3P:G4504280_3P_AT histone 1, H3a hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05834 Hypothetical protein ZK131.7 [Caenorhabditis elegans] emb|CAB05833.1| Hypothetical protein ZK131.3 [Caenorhabditis elegans] emb|CAB05831.1| Hypothetical protein ZK131.2 [Caenorhabditis elegans] emb|CAA97411.1| Hypothetical protein B0035.10 [Caenorhabditis elegans] emb|CAA92733.1| Hypothetical protein F22B3.2 [Caenorhabditis elegans] emb|CAB07653.1| Hypothetical protein T10C6.13 [Caenorhabditis elegans] emb|CAB05209.1| Hypothetical protein F54E12.1 [Caenorhabditis elegans] emb|CAB04057.1| Hypothetical protein F08G2.3 [Caenorhabditis elegans] gb|AAC05102.1| Histone protein 32 [Caenorhabditis elegans] gb|AAK84514.1| Histone protein 49 [Caenorhabditis elegans] gb|AAF98226.1| Histone protein 17 [Caenorhabditis elegans] gb|AAF98231.1| Histone protein 27 [Caenorhabditis elegans] emb|CAA33644.1| Histone protein [Caenorhabditis elegans] gb|AAB00650.1| Histone protein 59 [Caenorhabditis elegans] gb|AAC48033.1| Histone protein 6 [Caenorhabditis elegans] ref|NP_505292.1| HIStone family member (his-27) [Caenorhabditis elegans] ref|NP_505297.1| HIStone family member (his-17) [Caenorhabditis elegans] ref|NP_496890.1| HIStone family member (his-13) [Caenorhabditis elegans] ref|NP_505199.1| HIStone family member (his-6) [Caenorhabditis elegans] ref|NP_501204.1| F55G1.2 [Caenorhabditis elegans] ref|NP_502138.1| HIStone family member (his-55) [Caenorhabditis elegans] ref|NP_502153.1| HIStone family member (his-63) [Caenorhabditis elegans] ref|NP_496899.1| HIStone family member (his-42) [Caenorhabditis elegans] ref|NP_505276.1| HIStone family member (his-49) [Caenorhabditis elegans] ref|NP_502134.1| HIStone family member (his-45) [Caenorhabditis elegans] ref|NP_507033.1| HIStone family member (his-2) [Caenorhabditis elegans] ref|NP_501407.1| HIStone family member (his-32) [Caenorhabditis elegans] ref|NP_496895.1| HIStone family member (his-25) [Caenorhabditis elegans] ref|NP_496894.1| HIStone family member (his-9) [Caenorhabditis elegans] gb|AAG50235.1| histone H3 [Caenorhabditis elegans] pir||HSKW3 histone H3 - Caenorhabditis elegans 5.0E-70
blast P08898 Histone H3 1.0E-69
blast AAH74969 H3/o protein [Homo sapiens] 2.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007125 EMBL-EBI Histone core 1.6E-29
Sequence
Target Sequence 
>C. ELEGANS:172833_X_AT
aaagcagctggccaccaaggcagctcgcaagtctgctccagcctctggaggagtcaagaa
gccacatcgttaccgtccaggaaccgtcgctctccgtgagatcagacgttaccagaagtc
taccgagctcctcatccgcagagcgccattccaacgccttgttcgtgaaattgctcaaga
tttcaagaccgacctccgcttccaatcttccgctgtcatggctcttcaagaggctgccga
ggcttacctcgtcggactcttcgaggacaccaacttgtgcgcaatccacgccaagcgagt
caccatcatgccaaaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCAGCTGGCCACCAAGGCAGCT 615 123 66 Antisense
GTCTGCTCCAGCCTCTGGAGGAGTC 544 471 96 Antisense
GAGTCAAGAAGCCACATCGTTACCG 422 399 116 Antisense
CCGTCGCTCTCCGTGAGATCAGACG 533 265 149 Antisense
ACGTTACCAGAAGTCTACCGAGCTC 167 95 171 Antisense
TCCAACGCCTTGTTCGTGAAATTGC 279 587 215 Antisense
GCTCAAGATTTCAAGACCGACCTCC 2 303 238 Antisense
GGCTCTTCAAGAGGCTGCCGAGGCT 400 539 285 Antisense
TTACCTCGTCGGACTCTTCGAGGAC 550 681 309 Antisense
GAGGACACCAACTTGTGCGCAATCC 301 405 328 Antisense
GCGAGTCACCATCATGCCAAAGGAC 193 291 360 Antisense
Sequence Source Affymetrix Proprietary Database