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[172822_x_at]

Description C49D10.1 /REP_DB=WormBase Gene ID /WP=CE19380 /TR=O16610 /GB=AAC71186.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse transcriptase
Cluster 6grp_total > 09      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 40.7
(-2.9)
M 		40.1
(-3)
A 		44.7
(-2.4)
A 		86.6
(3)
P 		47
B 51.2
(-0.2)
A 		81.8
(3)
P 		24.5
(-3)
A 		41.7
(-1.2)
P 		57
C 59.8
(-2.4)
A 		70.2
(-2.1)
A 		42.2
(-3)
P 		221.7
(3)
P 		180
avr 50.6
(-2) 		64
(-1) 		37.1
(-3) 		116.7
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 106.8
(3)
A 		35.9
(-1.6)
A 		32.4
(-1.9)
P 		83.8
(1.5)
A 		51.9
(-0.6)
A 		14.8
(-3)
A 		92
B 61.3
(3)
M 		38.1
(0.4)
A 		8
(-3)
A 		21.6
(-1.5)
A 		43.4
(1)
A 		45.8
(1.3)
A 		53
C 32.9
(-0.8)
A 		35.7
(-0.5)
A 		8.3
(-3)
A 		73.9
(3)
A 		31.3
(-0.9)
A 		10.2
(-2.8)
A 		66
avr 67
(3) 		36.6
(-0.6) 		16.2
(-3) 		59.8
(2.1) 		42.2
(0.1) 		23.6
(-2.1) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 14.8
(3)
M 		8.7
(-3)
A 		11.7
(-0)
A 		12.7
(0.9)
P 		6
B 16.3
(1.4)
A 		20.2
(3)
P 		5.7
(-3)
A 		8.9
(-1.7)
P 		15
C 19.8
(-1.7)
A 		20.2
(-1.5)
A 		14.7
(-3)
P 		37.4
(3)
P 		23
avr 17
(1.2) 		16.4
(0.8) 		10.7
(-3) 		19.7
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 19.7
(2.5)
A 		12.8
(0.1)
A 		7.1
(-1.8)
P 		21.2
(3)
A 		15.7
(1.1)
A 		3.6
(-3)
A 		18
B 21.3
(3)
M 		12.7
(0.3)
A 		2
(-3)
A 		5.7
(-1.8)
A 		17.6
(1.8)
A 		17.1
(1.7)
A 		19
C 12.2
(1.7)
A 		10.9
(1.1)
A 		2.1
(-3)
A 		14.9
(3)
A 		10.1
(0.8)
A 		3.4
(-2.4)
A 		13
avr 17.7
(3) 		12.1
(0.6) 		3.7
(-3) 		13.9
(1.4) 		14.5
(1.6) 		8
(-1.2) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172822_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4233
Sequence Type Exemplar sequence
Representative Public ID C49D10.1 NCBI
Target Description C49D10.1 /REP_DB=WormBase Gene ID /WP=CE19380 /TR=O16610 /GB=AAC71186.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse transcriptase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062282(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062282 NCBI Caenorhabditis elegans C49D10.1 (C49D10.1) mRNA, complete cds. 11/11 None
SNAP00000027217 ENSEMBL cdna:SNAP chromosome:CEL140:II:3883827:3884805:1 11/11 None
C49D10.1 ENSEMBL cdna:known chromosome:CEL140:II:3882414:3884805:1 gene:C49D10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075796 3/11 Cross Hyb Matching Probes A 
NM_059224 5/11 Cross Hyb Matching Probes A 
SNAP00000009743 1/11 Cross Hyb Matching Probes None
M01A10.4 5/11 Cross Hyb Matching Probes A 
F22B8.8 3/11 Cross Hyb Matching Probes None
F55A4.6 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Public Domain and Genome References
Ensembl C49D10.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71186 Hypothetical protein C49D10.1 [Caenorhabditis elegans] ref|NP_494683.1| C49D10.1 [Caenorhabditis elegans] 0.0
blast AAB42276 Hypothetical protein M01A10.4 [Caenorhabditis elegans] ref|NP_491625.1| M01A10.4 [Caenorhabditis elegans] 0.0
blast NP_510671 F38E9.3 [Caenorhabditis elegans] 0.0
blast AAC71186 Hypothetical protein C49D10.1 [Caenorhabditis elegans] ref|NP_494683.1| C49D10.1 [Caenorhabditis elegans] 1.0E-170
blast AAB42276 Hypothetical protein M01A10.4 [Caenorhabditis elegans] ref|NP_491625.1| M01A10.4 [Caenorhabditis elegans] 1.0E-163
blast AAA21080 Hypothetical protein T07E3.1 [Caenorhabditis elegans] ref|NP_498506.1| T07E3.1 [Caenorhabditis elegans] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.0E-18
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.3E-30
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 2.8E-26
Sequence
Target Sequence 
>C. ELEGANS:172822_X_AT
ttatctcaatctcaaatgccgctttactgaaatgccgccagctgcttcgatcattccagt
cttccaatgctcaattctatttcaaactgtacaacacctatgtcagaccaatcctggaat
atggttgcgagatttttcacccgtccactattgccctttcagataaactagagtccccac
ttcgcttcttttcacgtaaagtgtttcaaagatgtaatctgaaattctcatcctattatg
atagactttcccaatttgaattgaaaagcaccctccacagaagactcctcatgattctta
aaacattctacaacattgtcacaaagtcatatcattttccatcattattcctctatgtta
aatgtcctcgttccaaaagatttccatatatgttatgttcaactggtataccaaataagg
ggttactacatgctaacctgtcattatggaaccacgttgctagactgttgcctaaaataa
tgagtccatcggcttttgctgcgcgtattaattccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATCTCAATCTCAAATGCCGCTTT 607 679 1742 Antisense
GCCGCTTTACTGAAATGCCGCCAGC 420 285 1759 Antisense
TCATTCCAGTCTTCCAATGCTCAAT 575 619 1792 Antisense
ATGGTTGCGAGATTTTTCACCCGTC 101 51 1862 Antisense
CCTCCACAGAAGACTCCTCATGATT 293 267 2013 Antisense
TCATTTTCCATCATTATTCCTCTAT 670 619 2073 Antisense
ATGTCCTCGTTCCAAAAGATTTCCA 237 49 2103 Antisense
GGGGTTACTACATGCTAACCTGTCA 334 497 2160 Antisense
CCACGTTGCTAGACTGTTGCCTAAA 317 273 2193 Antisense
TAATGAGTCCATCGGCTTTTGCTGC 640 635 2219 Antisense
TTTTGCTGCGCGTATTAATTCCATC 53 679 2235 Antisense
Sequence Source Affymetrix Proprietary Database