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[172814_x_at]

Description C35E7.10B /REP_DB=WormBase Gene ID /WP=CE17524 /TR=O61768 /GB=AAC17526.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 78      4grp_total > 43      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 1.4
(-3)
A 		3.6
(1.3)
A 		3.1
(0.3)
A 		4.5
(3)
A 		3
B 3.5
(0.9)
A 		2.8
(-1.6)
A 		2.4
(-3)
A 		4.1
(3)
A 		2
C 1.4
(-3)
A 		17.4
(3)
A 		2.2
(-2.7)
A 		2.2
(-2.7)
A 		16
avr 2.1
(-3) 		7.9
(3) 		2.6
(-2.5) 		3.6
(-1.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 16.5
(-1.3)
A 		1.6
(-3)
A 		2.9
(-2.9)
A 		2.6
(-2.9)
A 		13.7
(-1.7)
A 		55.7
(3)
A 		54
B 6
(-2.6)
A 		1.4
(-3)
A 		1.8
(-3)
A 		7.4
(-2.5)
A 		42.7
(0.4)
A 		74.3
(3)
P 		73
C 3.1
(-3)
A 		9.4
(-2.4)
A 		6.2
(-2.7)
A 		8.4
(-2.5)
A 		37.4
(0.3)
A 		66.1
(3)
M 		63
avr 8.5
(-2.5) 		4.1
(-3) 		3.6
(-3) 		6.1
(-2.8) 		31.3
(-0.3) 		65.4
(3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.5
(-3)
A 		0.8
(3)
A 		0.8
(3)
A 		0.7
(1)
A 		0
B 1.1
(3)
A 		0.7
(-1.8)
A 		0.6
(-3)
A 		0.9
(0.6)
A 		1
C 0.5
(-2.9)
A 		5
(3)
A 		0.8
(-2.5)
A 		0.4
(-3)
A 		5
avr 0.7
(-3) 		2.2
(3) 		0.7
(-3) 		0.7
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 3.1
(-1.8)
A 		0.6
(-3)
A 		0.6
(-3)
A 		0.7
(-3)
A 		4.1
(-1.4)
A 		13.6
(3)
A 		13
B 2.1
(-2.6)
A 		0.5
(-3)
A 		0.4
(-3)
A 		2
(-2.6)
A 		17.4
(0.7)
A 		27.7
(3)
P 		27
C 1.2
(-3)
A 		2.9
(-2.5)
A 		1.6
(-2.9)
A 		1.7
(-2.9)
A 		12.1
(0.1)
A 		22.1
(3)
M 		21
avr 2.1
(-2.6) 		1.3
(-2.9) 		0.9
(-3) 		1.5
(-2.8) 		11.2
(0.1) 		21.1
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172814_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1574
Sequence Type Exemplar sequence
Representative Public ID C35E7.10B NCBI
Target Description C35E7.10B /REP_DB=WormBase Gene ID /WP=CE17524 /TR=O61768 /GB=AAC17526.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060425(11), NM_060426(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060425 NCBI Caenorhabditis elegans C35E7.10a (C35E7.10) mRNA, complete cds. 11/11 A 
NM_060426 NCBI Caenorhabditis elegans C35E7.10b (C35E7.10) mRNA, complete cds. 11/11 None
GENEFINDER00000013387 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10818251:10820894:-1 11/11 None
SNAP00000013370 ENSEMBL cdna:SNAP chromosome:CEL140:I:10818823:10820894:-1 11/11 None
C35E7.10a ENSEMBL cdna:known chromosome:CEL140:I:10818823:10820894:-1 gene:C35E7.10 11/11 A 
C35E7.10b ENSEMBL cdna:known chromosome:CEL140:I:10818828:10820978:-1 gene:C35E7.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068445 5/11 Cross Hyb Matching Probes A 
SNAP00000018110 5/11 Cross Hyb Matching Probes None
GENEFINDER00000018125 5/11 Cross Hyb Matching Probes None
C55C3.4 5/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10819608-10820825(-) 100.0 100.0
chrIV:5676855-5678043(-) 96.45 96.45
Public Domain and Genome References
Ensembl C35E7.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC17525 Hypothetical protein C35E7.10a [Caenorhabditis elegans] ref|NP_492826.1| C35E7.10a [Caenorhabditis elegans] 0.0
blast NP_500846 C55C3.4 [Caenorhabditis elegans] gb|AAA96169.1| Hypothetical protein C55C3.4 [Caenorhabditis elegans] 0.0
blast CAE63909 Hypothetical protein CBG08480 [Caenorhabditis briggsae] 1.0E-179
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-13
hanks 2.1.6 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CamKI 1.0E-61
hanks 1.6.2 AGC group; AGC VI phosphorylate ribosomal protein S6; RSK1N 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 2.1E-15
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-57
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-32
Sequence
Target Sequence 
>C. ELEGANS:172814_X_AT
gacttctgctaatcactttgttgttcgtgagagtgatcacaaagtctacgttgataagat
tggcttcccaacaatcatcgaactcgtcaatcactatgtgtcgaccaaagagagcttctc
gaccaaggatggaactgtcaaggctgtgctgaagcaggctgtggagcgtcagaaatggga
gctgtaccatgaggatgtggagttgacgaagaagcttggagagggagcatttggagaagt
atggaaggggaagctcctgaagattctggatgcaaatcatcagcctgtacttgtcgctgt
gaagactgcaaagctggagagtatgacgaaggagcagatcaaggagattatgcgggaggc
tcgattgatgagaaatcttgatcatatcaacgtcgtcaaattcttcggagtagcagctgg
cacggaacctctttacgtgatcatggagttggctgatggtggagcactcgattctgccct
tgggaagctccatttcccgatgatcaagaagtacgagttgatctaccaagctgccaatgg
tctcgcctacatccacgagaagaacctgatgcaccgggatgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTCTGCTAATCACTTTGTTGTT 492 371 177 Antisense
TGGCTTCCCAACAATCATCGAACTC 352 545 237 Antisense
TCGAACTCGTCAATCACTATGTGTC 630 599 254 Antisense
GGATGGAACTGTCAAGGCTGTGCTG 287 515 303 Antisense
AAATCATCAGCCTGTACTTGTCGCT 676 115 450 Antisense
AATTCTTCGGAGTAGCAGCTGGCAC 591 175 575 Antisense
CGGAACCTCTTTACGTGATCATGGA 547 241 599 Antisense
TCGATTCTGCCCTTGGGAAGCTCCA 247 603 644 Antisense
AGCTCCATTTCCCGATGATCAAGAA 338 81 662 Antisense
ACGAGTTGATCTACCAAGCTGCCAA 429 93 689 Antisense
GAAGAACCTGATGCACCGGGATGTC 680 339 735 Antisense
Sequence Source Affymetrix Proprietary Database