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[172813_x_at] DNAJ protein like

Description B0035.2 /REP_DB=WormBase Gene ID /WP=CE05160 /TR=Q17433 /GB=CAA97409.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DNAJ protein like
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 61      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 63      6grp_total > 60      4grp_total > 50      6grp_highest_sub > 03_04      6grp_cgc > 94      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 333.5
(-2.3)
P 		530.7
(3)
P 		432
(0.3)
P 		307.5
(-3)
P 		223
B 340
(-2)
P 		593.1
(3)
P 		456
(0.3)
P 		291.9
(-3)
P 		301
C 304.8
(-3)
P 		394.3
(1.1)
P 		434.9
(3)
P 		310.8
(-2.7)
P 		130
avr 326.1
(-2.3) 		506
(3) 		441
(1.1) 		303.4
(-3) 		203

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 626
1 2 3 4 5 6 range
A 508
(-2.4)
P 		543.3
(-1.9)
P 		846.9
(2.4)
P 		885.8
(3)
P 		526.9
(-2.2)
P 		469.8
(-3)
P 		416
B 628.4
(-1)
P 		539.4
(-2.6)
P 		784.3
(2)
P 		837
(3)
P 		555.6
(-2.3)
P 		520.9
(-3)
P 		316
C 625.7
(-2.4)
P 		644.3
(-2.2)
P 		630.9
(-2.3)
P 		1095.8
(3)
P 		574.2
(-3)
P 		586.5
(-2.9)
P 		522
avr 587.4
(-2.1) 		575.7
(-2.3) 		754
(0.3) 		939.5
(3) 		552.2
(-2.6) 		525.7
(-3) 		414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 121.4
(3)
P 		115.2
(2.5)
P 		112.9
(2.3)
P 		45
(-3)
P 		76
B 108.4
(0.3)
P 		146.5
(3)
P 		105.7
(0.1)
P 		62.2
(-3)
P 		84
C 100.9
(-0.1)
P 		113.4
(0.7)
P 		151.6
(3)
P 		52.4
(-3)
P 		99
avr 110.2
(1.8) 		125
(3) 		123.4
(2.9) 		53.2
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 93.6
(-3)
P 		193.2
(1.6)
P 		184.4
(1.2)
P 		224.7
(3)
P 		159
(-0)
P 		114.4
(-2)
P 		131
B 218.6
(2.1)
P 		179.6
(-3)
P 		190.5
(-1.6)
P 		221.7
(2.5)
P 		225.7
(3)
P 		194.3
(-1.1)
P 		46
C 231.9
(3)
P 		196.6
(0)
P 		160.6
(-3)
P 		220.4
(2)
P 		185.8
(-0.9)
P 		196.4
(0)
P 		71
avr 181.4
(-1.6) 		189.8
(-0.6) 		178.5
(-1.9) 		222.3
(3) 		190.2
(-0.6) 		168.4
(-3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172813_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9035
Sequence Type Exemplar sequence
Representative Public ID B0035.2 NCBI
Target Description B0035.2 /REP_DB=WormBase Gene ID /WP=CE05160 /TR=Q17433 /GB=CAA97409.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DNAJ protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069725(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069725 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-2) (dnj-2) mRNA, complete cds. 11/11 A 
SNAP00000035831 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11312685:11313947:-1 11/11 None
GENEFINDER00000035854 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11312685:11313947:-1 11/11 None
B0035.2 ENSEMBL cdna:known chromosome:CEL140:IV:11312444:11313951:-1 gene:B0035.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070247 5/11 Cross Hyb Matching Probes A 
SNAP00000017712 6/11 Cross Hyb Matching Probes None
GENEFINDER00000017723 6/11 Cross Hyb Matching Probes None
C47A4.1 5/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11312690-11313953(-) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ protein like
Gene Symbol dnj-2
Ensembl B0035.2
Entrez Gene 178043 Entrez gene
WormBase CE05160 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264427_AT DNAJ heat shock N-terminal domain-containing protein at
ATGENOME1:14136_AT DNAJ heat shock N-terminal domain-containing protein at
CANINE_2:CFAAFFX.5449.1.S1_AT similar to CG7872-PA cfa
DROSOPHILA_2:1636142_AT dm
DROSGENOME1:154301_AT dm
MG-U74BV2:107789_AT RIKEN cDNA 2010203O07 gene mm
MG-U74CV2:130910_AT RIKEN cDNA 2010203O07 gene mm
MG-U74CV2:140873_R_AT PREDICTED: hypothetical protein LOC72429 [Mus musculus], mRNA sequence mm
Protein Similarities
Method ID Description E-Value
blast CAA97409 Hypothetical protein B0035.2 [Caenorhabditis elegans] ref|NP_502126.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-2) [Caenorhabditis elegans] sp|Q17433|DNJ2_CAEEL DnaJ homolog dnj-2 precursor (DnaJ domain protein 2) 0.0
blast CAE62051 Hypothetical protein CBG06069 [Caenorhabditis briggsae] 1.0E-159
blast CAB62792 Hypothetical protein C47A4.1 [Caenorhabditis elegans] ref|NP_502648.1| C47A4.1 [Caenorhabditis elegans] 8.0E-91
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.99999996490334E-14
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 4.3E-22
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502126.1 1 129-148
B0035.2 1 129-148
SNAP00000035831 1 129-148
GENEFINDER00000035854 1 129-148
Sequence
Target Sequence 
>C. ELEGANS:172813_X_AT
aactattcttatcatttcactcttccaattcctctctgcaaaacacaagttctcagaagc
aatcgaatatgcaaccggtgttggaaaattccgcaatatggcaattaaagatggaattga
taaaggcttgctggaaatggatcggaatggaaaattaaaaaagaacaaaggagttgacaa
tgatgaagttatcaagcaaataattattgataatcttgatgtcacgggtggctacaaacg
agaatccatctacgatactctcgcatggcacacaattattttcccattaacgatattccg
atacattaaatggacagcactttggtattggagatttgcaattcaaaaggaggaatatga
tgatgacgccaagctttatctcattagaaagtacattggagtttcacaaatggaattcga
tcagaagtacactgatgaagacattgatgatctttttgaacgagaatgctggctgaaacg
aaactgtgcaacgtggaaagccgaacgagacgctgcggaacaagaaaaaatggctcaatc
gggtcggtacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTATTCTTATCATTTCACTCTTC 4 141 417 Antisense
TCCAATTCCTCTCTGCAAAACACAA 530 587 440 Antisense
GTCACGGGTGGCTACAAACGAGAAT 306 457 637 Antisense
GAATCCATCTACGATACTCTCGCAT 323 329 658 Antisense
GATACTCTCGCATGGCACACAATTA 667 423 670 Antisense
GGCACACAATTATTTTCCCATTAAC 600 535 683 Antisense
AATGGACAGCACTTTGGTATTGGAG 6 163 725 Antisense
GACGCCAAGCTTTATCTCATTAGAA 710 379 781 Antisense
GAATGCTGGCTGAAACGAAACTGTG 438 331 880 Antisense
AAGCCGAACGAGACGCTGCGGAACA 138 147 914 Antisense
AAATGGCTCAATCGGGTCGGTACAA 584 119 944 Antisense
Sequence Source Affymetrix Proprietary Database