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[172811_x_at]

Description C35E7.10A /REP_DB=WormBase Gene ID /WP=CE17523 /TR=O61767 /GB=AAC17525.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 79      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 7.2
(-3)
A 		67.7
(2.5)
A 		21.7
(-1.7)
A 		73.2
(3)
A 		66
B 21.4
(-2)
A 		7.5
(-3)
A 		32.2
(-1.2)
A 		88.4
(3)
A 		81
C 9.6
(-2.7)
A 		30.1
(-1.4)
A 		4.4
(-3)
A 		99.4
(3)
P 		95
avr 12.7
(-3) 		35.1
(-1.2) 		19.4
(-2.5) 		87
(3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 86.7
(-0.7)
M 		22.5
(-2.5)
A 		41.1
(-2)
A 		5.2
(-3)
A 		90.7
(-0.6)
A 		216.3
(3)
P 		211
B 21.7
(-2.4)
A 		59.9
(-1.1)
A 		6.6
(-3)
A 		5.6
(-3)
A 		95.4
(0.2)
P 		173.1
(3)
P 		168
C 9.3
(-2.9)
A 		44.5
(-1.6)
A 		31.5
(-2.1)
A 		7.1
(-3)
A 		98.2
(0.4)
A 		169.7
(3)
P 		163
avr 39.2
(-1.9) 		42.3
(-1.8) 		26.4
(-2.3) 		6
(-3) 		94.8
(-0) 		186.4
(3) 		180
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 2.6
(-3)
A 		14.7
(3)
A 		5.7
(-1.5)
A 		10.7
(1)
A 		12
B 6.8
(-1.2)
A 		1.8
(-3)
A 		7.4
(-1)
A 		18.8
(3)
A 		17
C 3.2
(-2.3)
A 		8.6
(-0.2)
A 		1.5
(-3)
A 		16.8
(3)
P 		15
avr 4.2
(-3) 		8.4
(-0.8) 		4.9
(-2.6) 		15.4
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 16
(-1.3)
M 		8
(-2.2)
A 		9
(-2.1)
A 		1.3
(-3)
A 		27.4
(0)
A 		52.7
(3)
P 		51
B 7.6
(-2.4)
A 		19.9
(-1.2)
A 		1.6
(-3)
A 		1.5
(-3)
A 		38.8
(0.6)
P 		64.5
(3)
P 		63
C 3.5
(-2.8)
A 		13.6
(-1.7)
A 		8
(-2.3)
A 		1.4
(-3)
A 		31.8
(0.3)
A 		56.8
(3)
P 		55
avr 9
(-2.2) 		13.8
(-1.7) 		6.2
(-2.5) 		1.4
(-3) 		32.7
(0.3) 		58
(3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172811_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1553
Sequence Type Exemplar sequence
Representative Public ID C35E7.10A NCBI
Target Description C35E7.10A /REP_DB=WormBase Gene ID /WP=CE17523 /TR=O61767 /GB=AAC17525.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060425(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060425 NCBI Caenorhabditis elegans C35E7.10a (C35E7.10) mRNA, complete cds. 11/11 A 
GENEFINDER00000013387 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10818251:10820894:-1 11/11 None
SNAP00000013370 ENSEMBL cdna:SNAP chromosome:CEL140:I:10818823:10820894:-1 11/11 None
C35E7.10a ENSEMBL cdna:known chromosome:CEL140:I:10818823:10820894:-1 gene:C35E7.10 11/11 A 
C35E7.10b ENSEMBL cdna:known chromosome:CEL140:I:10818828:10820978:-1 gene:C35E7.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_060426 3/11 Cross Hyb Matching Probes A 
NM_068445 4/11 Cross Hyb Matching Probes A 
SNAP00000018110 4/11 Cross Hyb Matching Probes None
GENEFINDER00000018125 4/11 Cross Hyb Matching Probes None
C55C3.4 4/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172988 2 1 NM_060425 172811_x_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5676202-5678043(-) 96.75 96.75
chrI:10818753-10820825(-) 100.0 100.0
Public Domain and Genome References
Ensembl C35E7.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC17525 Hypothetical protein C35E7.10a [Caenorhabditis elegans] ref|NP_492826.1| C35E7.10a [Caenorhabditis elegans] 0.0
blast NP_500846 C55C3.4 [Caenorhabditis elegans] gb|AAA96169.1| Hypothetical protein C55C3.4 [Caenorhabditis elegans] 0.0
blast CAE63909 Hypothetical protein CBG08480 [Caenorhabditis briggsae] 1.0E-179
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-13
hanks 2.1.6 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CamKI 1.0E-61
hanks 1.6.2 AGC group; AGC VI phosphorylate ribosomal protein S6; RSK1N 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 2.1E-15
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-57
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-32
Sequence
Target Sequence 
>C. ELEGANS:172811_X_AT
acgagttgatctaccaagctgccaatggtctcgcctacatccacgagaagaacctgatgc
accgggatgtcgctgctcgtaactgcctttatggtggtggacaggtcaagatcgctgatt
tcggactgtcccgtgaaggtgcggactacacgatggatctgaaaaagaaggtaccaatcc
gttggttgcctccagagaccattcgttcgggtctttatactccaaagactgacgtcttcg
cttttggagtgatggcatgggaaatcaccgaggatgggaaggagccatatcctggattca
aggttattgaggtggcaatgaaggttctccaaggataccgtatgccgttcagcccggagg
taaacaaggagtttgcagaattcatcttgacgaaatgctggccggaaaagtatgaggatc
gtctcccaatgaaggacatttgcaagtggatgaagtcgtttgtcaaggctcaaggaggag
atactggtccaggcggaatgatgtcgaagattggcaagaaaatgaaacggtcaaactcgc
gtgagaagagcaaaagctcgactatgactgctgcacatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAGTTGATCTACCAAGCTGCCAA 430 93 689 Antisense
GAAGAACCTGATGCACCGGGATGTC 681 339 735 Antisense
GTAACTGCCTTTATGGTGGTGGACA 21 463 767 Antisense
AAGATCGCTGATTTCGGACTGTCCC 86 155 796 Antisense
GGACTGTCCCGTGAAGGTGCGGACT 613 519 811 Antisense
CATTCGTTCGGGTCTTTATACTCCA 260 209 888 Antisense
TGACGTCTTCGCTTTTGGAGTGATG 172 571 918 Antisense
GGTTCTCCAAGGATACCGTATGCCG 450 507 1011 Antisense
TGCCGTTCAGCCCGGAGGTAAACAA 680 583 1031 Antisense
GAGGATCGTCTCCCAATGAAGGACA 653 403 1102 Antisense
AGCTCGACTATGACTGCTGCACATG 160 81 1243 Antisense
Sequence Source Affymetrix Proprietary Database