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[172806_x_at] carboxylesterase

Description B0238.13 /REP_DB=WormBase Gene ID /WP=CE27566 /TR=O16496 /GB=AAB65992.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=carboxylesterase
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 85      4grp_high_8_8 > 05      6grp_highest_nonorm > 91      6grp_total > 65      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 111.2
(-1.7)
P 		110.6
(-1.7)
P 		87.2
(-3)
A 		196.1
(3)
P 		109
B 75
(-3)
P 		97.3
(-2.1)
P 		84.3
(-2.6)
P 		230
(3)
P 		155
C 105.3
(-1.8)
P 		46.8
(-3)
P 		78.3
(-2.4)
P 		346.2
(3)
P 		299
avr 97.2
(-2.5) 		84.9
(-2.9) 		83.3
(-3) 		257.4
(3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 151.3
(-3)
P 		184.9
(-1.4)
P 		245.6
(1.4)
P 		234.6
(0.9)
P 		260.4
(2.1)
P 		279
(3)
P 		128
B 192.9
(-3)
P 		200.7
(-2.6)
P 		309
(2.6)
P 		205.9
(-2.4)
P 		240.8
(-0.7)
P 		316.4
(3)
P 		124
C 177.8
(-3)
P 		186.8
(-2.6)
P 		256.1
(0.2)
P 		213.4
(-1.6)
P 		208.4
(-1.8)
P 		326.4
(3)
P 		149
avr 174
(-3) 		190.8
(-2.2) 		270.2
(1.3) 		218
(-1) 		236.5
(-0.2) 		307.3
(3) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 40.5
(3)
P 		24
(-2.6)
P 		22.8
(-3)
A 		28.7
(-1)
P 		18
B 23.9
(-2.1)
P 		24
(-2.1)
P 		19.5
(-3)
P 		49
(3)
P 		30
C 34.9
(-0.1)
P 		13.5
(-3)
P 		27.3
(-1.2)
P 		58.4
(3)
P 		45
avr 33.1
(0) 		20.5
(-3) 		23.2
(-2.3) 		45.4
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 27.9
(-3)
P 		65.8
(1.5)
P 		53.5
(0)
P 		59.5
(0.7)
P 		78.6
(3)
P 		67.9
(1.7)
P 		51
B 67.1
(-1.8)
P 		66.8
(-1.8)
P 		75
(-1.1)
P 		54.5
(-3)
P 		97.8
(1.1)
P 		118
(3)
P 		64
C 65.9
(-0.9)
P 		57
(-1.7)
P 		65.2
(-1)
P 		42.9
(-3)
P 		67.4
(-0.8)
P 		109.3
(3)
P 		66
avr 53.6
(-2.8) 		63.2
(-1.6) 		64.6
(-1.4) 		52.3
(-3) 		81.3
(0.8) 		98.4
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172806_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11286
Sequence Type Exemplar sequence
Representative Public ID B0238.13 NCBI
Target Description B0238.13 /REP_DB=WormBase Gene ID /WP=CE27566 /TR=O16496 /GB=AAB65992.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=carboxylesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072000(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072000 NCBI Caenorhabditis elegans B0238.13 (carboxylesterase) mRNA, complete cds. 10/11 A 
SNAP00000038868 ENSEMBL cdna:SNAP chromosome:CEL140:V:5243555:5249374:-1 11/11 None
GENEFINDER00000038878 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5243435:5247833:-1 11/11 None
B0238.13 ENSEMBL cdna:known chromosome:CEL140:V:5243541:5247833:-1 gene:B0238.13 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071997 6/11 Cross Hyb Matching Probes A 
NM_071999 1/11 Cross Hyb Matching Probes A 
SNAP00000017568 6/11 Cross Hyb Matching Probes None
GENEFINDER00000017570 4/11 Cross Hyb Matching Probes None
SNAP00000038867 1/11 Cross Hyb Matching Probes None
GENEFINDER00000038879 1/11 Cross Hyb Matching Probes None
C52A10.2 6/11 Cross Hyb Matching Probes A 
B0238.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5243554-5245298(-) 99.85 99.85
chrV:5234640-5242362(-) 95.34 95.34
chrV:5235665-5236435(-) 48.78 48.78
Public Domain and Genome References
Gene Title carboxylesterase
Ensembl B0238.13
Entrez Gene 178913 Entrez gene
SwissProt O16496 EMBL-EBI
WormBase CE29530 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65992 Hypothetical protein B0238.13 [Caenorhabditis elegans] ref|NP_504401.1| B0238.13 [Caenorhabditis elegans] 0.0
blast AAK68262 Hypothetical protein C52A10.2 [Caenorhabditis elegans] ref|NP_504398.1| C52A10.2 [Caenorhabditis elegans] 0.0
blast AAB65983 Hypothetical protein B0238.1 [Caenorhabditis elegans] ref|NP_504400.1| B0238.1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 2.5E-28
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 4.6E-122
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000038868 3 11-30,57-79,96-118
Sequence
Target Sequence 
>C. ELEGANS:172806_X_AT
gaaaacccctcaacaaatgctatcagcagctttgggtcctgatgttgtgaagaataggga
agaagttgtcaagaaagctgaagaatcttatttaaaaaatgctggaaacggaaatgaaaa
aaacgttgcaaagacattagttgagatatatggagactactacttcagtgtgggagcatt
tgatactgttcgatcacttgtcaagtatggaaatactgtatatttgtattctttcaatta
tttcaatccgaacactccaattgtgtttggcaaaaacaggccgttcaatgcaggtaatca
tggttctgatttaagatatatttttggagaagggactggagagttttcgaaagaagatag
gcaggttatggatatgatgggaacttatcaaacaaattttgcaaaatatggcaatccaaa
tggaaaaggtgtatcggttccatgggaaaagtacagtcttgacaatccaggaagatattt
caagattgattatccaaaatgtgatatggctgacaatttcttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACCCCTCAACAAATGCTATCA 329 355 702 Antisense
AATGCTATCAGCAGCTTTGGGTCCT 215 165 717 Antisense
AGCTTTGGGTCCTGATGTTGTGAAG 593 77 729 Antisense
GAGACTACTACTTCAGTGTGGGAGC 511 393 854 Antisense
GTGTGGGAGCATTTGATACTGTTCG 120 489 869 Antisense
GATACTGTTCGATCACTTGTCAAGT 687 423 883 Antisense
ATTATTTCAATCCGAACACTCCAAT 382 13 938 Antisense
AACAGGCCGTTCAATGCAGGTAATC 485 137 976 Antisense
GAAAAGGTGTATCGGTTCCATGGGA 659 347 1124 Antisense
AGTACAGTCTTGACAATCCAGGAAG 24 63 1151 Antisense
GTGATATGGCTGACAATTTCTTGGA 353 485 1202 Antisense
Sequence Source Affymetrix Proprietary Database