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[172800_x_at]

Description T22H2.6A /REP_DB=WormBase Gene ID /WP=CE24004 /TR=Q9U362 /GB=CAB54304.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=granulin precursor
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 55      6grp_total > 61      4grp_total > 04      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 901
1 2 3 4 range
A 1570.7
(-2)
P 		1429.1
(-2.9)
P 		1417.9
(-3)
P 		2318.7
(3)
P 		901
B 1642.1
(-1.2)
P 		1432.9
(-3)
P 		1781.1
(-0)
P 		2140.3
(3)
P 		707
C 1671.4
(0)
P 		1521
(-1.8)
P 		1419.6
(-3)
P 		1917
(3)
P 		497
avr 1628.1
(-1.5) 		1461
(-3) 		1539.5
(-2.3) 		2125.3
(3) 		664

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 905
1 2 3 4 5 6 range
A 1423.7
(-2)
P 		1483.1
(-1.6)
P 		2046.6
(3)
P 		1764.1
(0.7)
P 		1517.9
(-1.3)
P 		1303.8
(-3)
P 		743
B 1425.6
(-0.8)
P 		1298.9
(-1.8)
P 		1687.1
(1.3)
P 		1905.7
(3)
P 		1630.6
(0.8)
P 		1141.4
(-3)
P 		764
C 1526.8
(-0.2)
P 		1238.4
(-3)
P 		1850.2
(3)
P 		1664.1
(1.2)
P 		1662.8
(1.2)
P 		1297.4
(-2.4)
P 		612
avr 1458.7
(-0.9) 		1340.1
(-2.1) 		1861.3
(3) 		1778
(2.2) 		1603.8
(0.5) 		1247.5
(-3) 		614
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 262
1 2 3 4 range
A 571.9
(3)
P 		310.2
(-3)
P 		370.7
(-1.6)
P 		339.4
(-2.3)
P 		262
B 523.7
(3)
P 		353.9
(-3)
P 		412.7
(-0.9)
P 		456.1
(0.6)
P 		170
C 553.3
(3)
P 		437.3
(-0)
P 		494.8
(1.5)
P 		323.2
(-3)
P 		230
avr 549.6
(3) 		367.1
(-3) 		426.1
(-1.1) 		372.9
(-2.8) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 400
1 2 3 4 5 6 range
A 262.4
(-3)
P 		527.5
(3)
P 		445.7
(1.1)
P 		447.4
(1.2)
P 		458
(1.4)
P 		317.4
(-1.8)
P 		265
B 496
(-1)
P 		432.4
(-2.5)
P 		409.7
(-3)
P 		504.7
(-0.7)
P 		662.5
(3)
P 		425.6
(-2.6)
P 		253
C 565.9
(3)
P 		377.9
(-1.9)
P 		470.9
(0.5)
P 		334.6
(-3)
P 		538.1
(2.3)
P 		434.3
(-0.4)
P 		231
avr 441.4
(-1.2) 		445.9
(-1) 		442.1
(-1.1) 		428.9
(-1.6) 		552.9
(3) 		392.4
(-3) 		161
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172800_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1764
Sequence Type Exemplar sequence
Representative Public ID T22H2.6A NCBI
Target Description T22H2.6A /REP_DB=WormBase Gene ID /WP=CE24004 /TR=Q9U362 /GB=CAB54304.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=granulin precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060580(11), NM_060581(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060580 NCBI Caenorhabditis elegans T22H2.6a (T22H2.6) mRNA, complete cds. 11/11 A 
NM_060581 NCBI Caenorhabditis elegans T22H2.6b (T22H2.6) mRNA, complete cds. 10/11 A 
GENEFINDER00000038049 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11707291:11711715:1 11/11 None
SNAP00000038045 ENSEMBL cdna:SNAP chromosome:CEL140:I:11709987:11711715:1 11/11 None
T22H2.6a ENSEMBL cdna:known chromosome:CEL140:I:11709753:11711804:1 gene:T22H2.6 11/11 A 
T22H2.6b ENSEMBL cdna:known chromosome:CEL140:I:11709753:11711741:1 gene:T22H2.6 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018817 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11709683-11711646(+) 100.0 100.0
Public Domain and Genome References
Ensembl T22H2.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54304 Hypothetical protein T22H2.6a [Caenorhabditis elegans] ref|NP_492981.1| T22H2.6a [Caenorhabditis elegans] 0.0
blast E87929 protein T22H2.6 [imported] - Caenorhabditis elegans 0.0
blast CAB54305 Hypothetical protein T22H2.6b [Caenorhabditis elegans] ref|NP_492982.1| T22H2.6b [Caenorhabditis elegans] 0.0
blast CAB54305 Hypothetical protein T22H2.6b [Caenorhabditis elegans] ref|NP_492982.1| T22H2.6b [Caenorhabditis elegans] 1.0E-172
blast CAB54304 Hypothetical protein T22H2.6a [Caenorhabditis elegans] ref|NP_492981.1| T22H2.6a [Caenorhabditis elegans] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR000118 EMBL-EBI Granulin 1.5E-22
Pfam IPR000118 EMBL-EBI Granulin 3.9E-20
Pfam IPR000118 EMBL-EBI Granulin 3.7E-7
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.1E-26
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.1E-31
Pfam IPR000118 EMBL-EBI Granulin 1.5E-22
Pfam IPR000118 EMBL-EBI Granulin 3.9E-20
Pfam IPR000118 EMBL-EBI Granulin 3.7E-7
Pfam IPR000118 EMBL-EBI Granulin 1.5E-22
Pfam IPR000118 EMBL-EBI Granulin 3.9E-20
Pfam IPR000118 EMBL-EBI Granulin 3.7E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492981.1 2 21-40,69-91
NP_492982.1 2 21-40,69-91
T22H2.6a 2 21-40,69-91
T22H2.6b 2 21-40,69-91
GENEFINDER00000038049 4 15-37,116-135,174-196,217-239
Sequence
Target Sequence 
>C. ELEGANS:172800_X_AT
tcgtctgcccggacaaggctagcaagtgcccagatggttcgacgtgctgtctgttggagc
agggaagctacggatgttgcccggtgccgaatgcggtgtgctgcgcagatatgttgcact
gctgtccgaatggatttacgtgtcacgggcagttttgctcgcagaatttcgccatgatcc
cacacctgcggaagttcgcctccacctccatccatcgcaagccagcagtggagttccttt
ccgaagaagaggaggatgatgatgatgaggaatccacgtcatcagaggatccagaaagcg
atgtggacccgattgcctgtggagttggaaagacgtgtccagcaaaaaccacgtgctgta
atgtccgaggaaagaatggggagccgaagacgatgtgttgtccattgagcaacgccatct
gctgtgagaacacttgctgcccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTCTGCCCGGACAAGGCTAGCAA 44 599 542 Antisense
AAGTGCCCAGATGGTTCGACGTGCT 95 159 565 Antisense
GGGAAGCTACGGATGTTGCCCGGTG 223 493 603 Antisense
GATATGTTGCACTGCTGTCCGAATG 594 425 649 Antisense
TTACGTGTCACGGGCAGTTTTGCTC 416 681 677 Antisense
TGCTCGCAGAATTTCGCCATGATCC 486 581 697 Antisense
GCAAGCCAGCAGTGGAGTTCCTTTC 162 321 758 Antisense
GAAAGCGATGTGGACCCGATTGCCT 536 359 835 Antisense
AACCACGTGCTGTAATGTCCGAGGA 551 147 888 Antisense
TTGTCCATTGAGCAACGCCATCTGC 613 701 939 Antisense
TGCTGTGAGAACACTTGCTGCCCAG 306 581 961 Antisense
Sequence Source Affymetrix Proprietary Database