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[172798_x_at] Major sperm protein

Description ZK546.3 /REP_DB=WormBase Gene ID /WP=CE02917 /TR=Q23524 /GB=AAA68736.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster 6grp_total > 98      4grp_total > 41      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 27.7
(-3)
A 		152.5
(3)
A 		107.6
(0.8)
A 		37.7
(-2.5)
A 		125
B 118.3
(3)
A 		39.5
(-2.2)
A 		64.8
(-0.5)
A 		27.7
(-3)
A 		91
C 30.6
(-3)
A 		54.3
(-1.8)
A 		69.1
(-1.1)
A 		150.4
(3)
A 		120
avr 58.9
(-3) 		82.1
(3) 		80.5
(2.6) 		71.9
(0.4) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2913
1 2 3 4 5 6 range
A 8.2
(-3)
A 		24
(-3)
A 		87.4
(-2.8)
A 		255.3
(-2.5)
P 		1549.9
(0.3)
P 		2842.6
(3)
P 		2834
B 36.3
(-3)
A 		109.2
(-2.8)
A 		111.7
(-2.8)
A 		169
(-2.7)
A 		2110.2
(2.2)
P 		2431.6
(3)
P 		2395
C 21.6
(-3)
A 		121.2
(-2.8)
A 		90.2
(-2.9)
A 		150.4
(-2.7)
A 		2920.7
(3)
P 		2720.8
(2.6)
P 		2899
avr 22
(-3) 		84.8
(-2.9) 		96.4
(-2.8) 		191.6
(-2.6) 		2193.6
(1.9) 		2665
(3) 		2643
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 10.1
(-2)
A 		33.1
(3)
A 		28.1
(1.9)
A 		5.5
(-3)
A 		28
B 37.7
(3)
A 		9.8
(-2.3)
A 		15
(-1.3)
A 		5.9
(-3)
A 		32
C 10.1
(-3)
A 		15.6
(-0.8)
A 		24.1
(2.5)
A 		25.4
(3)
A 		15
avr 19.3
(1.2) 		19.5
(1.3) 		22.4
(3) 		12.3
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 944
1 2 3 4 5 6 range
A 1.5
(-3)
A 		8.5
(-2.9)
A 		19
(-2.8)
A 		64.7
(-2.5)
P 		467.7
(1.1)
P 		692.1
(3)
P 		691
B 12.6
(-3)
A 		36.3
(-2.8)
A 		27.1
(-2.9)
A 		44.8
(-2.8)
A 		857.3
(2.7)
P 		906.7
(3)
P 		894
C 8
(-3)
A 		37
(-2.8)
A 		22.9
(-2.9)
A 		30.2
(-2.9)
A 		945.1
(3)
P 		910.8
(2.8)
P 		937
avr 7.4
(-3) 		27.3
(-2.9) 		23
(-2.9) 		46.6
(-2.7) 		756.7
(2.4) 		836.5
(3) 		829
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172798_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4828
Sequence Type Exemplar sequence
Representative Public ID ZK546.3 NCBI
Target Description ZK546.3 /REP_DB=WormBase Gene ID /WP=CE02917 /TR=Q23524 /GB=AAA68736.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062505(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062505 NCBI Caenorhabditis elegans ZK546.3 (ZK546.3) mRNA, complete cds. 11/11 None
SNAP00000034948 ENSEMBL cdna:SNAP chromosome:CEL140:II:4939412:4940267:1 11/11 None
GENEFINDER00000034965 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4939341:4940267:1 10/11 None
ZK546.3 ENSEMBL cdna:known chromosome:CEL140:II:4939412:4940267:1 gene:ZK546.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062487 4/11 Cross Hyb Matching Probes A 
NM_068313 1/11 Cross Hyb Matching Probes None
NM_062558 1/11 Cross Hyb Matching Probes A 
NM_062559 2/11 Cross Hyb Matching Probes A 
NM_062569 1/11 Cross Hyb Matching Probes A 
NM_062490 2/11 Cross Hyb Matching Probes A 
NM_062500 2/11 Cross Hyb Matching Probes A 
NM_069380 1/11 Cross Hyb Matching Probes A 
NM_062464 4/11 Cross Hyb Matching Probes A 
NM_068372 1/11 Cross Hyb Matching Probes None
SNAP00000007649 4/11 Cross Hyb Matching Probes None
GENEFINDER00000007652 4/11 Cross Hyb Matching Probes None
SNAP00000016470 4/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 4/11 Cross Hyb Matching Probes None
SNAP00000019951 1/11 Cross Hyb Matching Probes None
SNAP00000019952 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019958 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019959 2/11 Cross Hyb Matching Probes None
SNAP00000021327 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021348 1/11 Cross Hyb Matching Probes None
SNAP00000034943 2/11 Cross Hyb Matching Probes None
GENEFINDER00000034961 2/11 Cross Hyb Matching Probes None
SNAP00000043868 4/11 Cross Hyb Matching Probes None
SNAP00000043869 2/11 Cross Hyb Matching Probes None
GENEFINDER00000043883 2/11 Cross Hyb Matching Probes None
GENEFINDER00000043888 4/11 Cross Hyb Matching Probes None
SNAP00000041533 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041543 1/11 Cross Hyb Matching Probes None
C33F10.9 4/11 Cross Hyb Matching Probes A 
R05F9.8 4/11 Cross Hyb Matching Probes A 
R05F9.3 2/11 Cross Hyb Matching Probes R A 
ZK546.6 2/11 Cross Hyb Matching Probes A 
F59A6.8 4/11 Cross Hyb Matching Probes None
F58A6.8 1/11 Cross Hyb Matching Probes A 
F58A6.9 1/11 Cross Hyb Matching Probes A 
C34F11.6 1/11 Cross Hyb Matching Probes A 
F32B6.6 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4939409-4940265(+) 100.0 100.0
chrII:4933004-4933393(+) 92.04 92.04
chrII:4825402-4825783(+) 90.05 90.05
chrII:4787147-4899172(+) 90.55 90.55
chrII:4892294-4892683(-) 93.53 93.53
chrII:5012855-5013244(-) 91.54 91.54
chrII:4916162-4916551(-) 91.04 91.04
chrII:5115081-5157067(-) 90.3 90.3
Public Domain and Genome References
Gene Title Major sperm protein
Ensembl ZK546.3
Entrez Gene 191344 Entrez gene
SwissProt Q23524 EMBL-EBI
WormBase CE02917 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68736 Hypothetical protein ZK546.3 [Caenorhabditis elegans] ref|NP_494906.1| ZK546.3 [Caenorhabditis elegans] 8.0E-75
blast AAA68736 Hypothetical protein ZK546.3 [Caenorhabditis elegans] ref|NP_494906.1| ZK546.3 [Caenorhabditis elegans] 1.0E-72
blast AAA68736 Hypothetical protein ZK546.3 [Caenorhabditis elegans] ref|NP_494906.1| ZK546.3 [Caenorhabditis elegans] 8.0E-72
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 5.0E-71
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 6.0E-71
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 9.0E-71
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 2.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.3E-33
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.3E-33
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.3E-33
Sequence
Target Sequence 
>C. ELEGANS:172798_X_AT
atgtttgttagattcgccagtgcccaatccgtcccaccaggagatatccagacccagccg
ggtacgaagatcgtgttcaatgcaccgtacgacgacaagcacacctaccacatcaaggtg
atcaactcctcggctcgccgtattggatacggtatcaagaccaccaatataaagagactt
ggagttgatccaccatgtggagttttggacccaaaggaagctgttcttcttgctgtttcc
tgcgatgccttcgctttcggacaggaggacactaacaacgatcgtatcactgttgagtgg
accaacactccggatggtgctgctaagcaattccgtcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTTGTTAGATTCGCCAGTGCCC 153 47 13 Antisense
TCCGTCCCACCAGGAGATATCCAGA 356 595 40 Antisense
ATATCCAGACCCAGCCGGGTACGAA 631 17 56 Antisense
GAAGATCGTGTTCAATGCACCGTAC 120 339 78 Antisense
ATGCACCGTACGACGACAAGCACAC 126 43 92 Antisense
ACATCAAGGTGATCAACTCCTCGGC 641 103 122 Antisense
ACTCCTCGGCTCGCCGTATTGGATA 100 99 137 Antisense
AGGAAGCTGTTCTTCTTGCTGTTTC 441 55 227 Antisense
ATGCCTTCGCTTTCGGACAGGAGGA 476 41 257 Antisense
ACCAACACTCCGGATGGTGCTGCTA 293 85 313 Antisense
TGGTGCTGCTAAGCAATTCCGTCGG 314 553 327 Antisense
Sequence Source Affymetrix Proprietary Database