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[172770_x_at] major sperm protein (MSP-53)

Description R13H9.4 /REP_DB=WormBase Gene ID /WP=CE02806 /GEN=msp-53 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=major sperm protein (MSP-53)
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 49      6grp_total > 98      4grp_total > 10      6grp_highest_sub > In      6grp_cgc > 79      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 402
1 2 3 4 range
A 156.4
(-3)
P 		215.5
(-2.1)
P 		227.8
(-1.9)
P 		558.1
(3)
P 		402
B 229.7
(-1.7)
P 		244.4
(-0.9)
P 		207.8
(-3)
P 		311.6
(3)
P 		104
C 228.6
(-2.7)
P 		250.5
(-2.3)
P 		211.6
(-3)
P 		545.8
(3)
P 		334
avr 204.9
(-3) 		236.8
(-2.3) 		215.7
(-2.8) 		471.8
(3) 		267

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14809
1 2 3 4 5 6 range
A 159.2
(-3)
P 		197.3
(-3)
P 		462.5
(-2.9)
P 		1240.8
(-2.6)
P 		10206.7
(1.1)
P 		14942.4
(3)
P 		14783
B 158.1
(-3)
P 		319.3
(-2.9)
P 		221
(-3)
P 		932.7
(-2.6)
P 		10436.3
(2.2)
P 		12024.5
(3)
P 		11866
C 133.6
(-3)
P 		571.1
(-2.8)
P 		191
(-3)
P 		955.9
(-2.6)
P 		12341
(3)
P 		11824.9
(2.7)
P 		12207
avr 150.3
(-3) 		362.6
(-2.9) 		291.5
(-2.9) 		1043.1
(-2.6) 		10994.7
(2.1) 		12930.6
(3) 		12780
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 56.9
(-1.3)
P 		46.8
(-3)
P 		59.5
(-0.8)
P 		81.7
(3)
P 		35
B 73.2
(3)
P 		60.4
(-0.1)
P 		48.1
(-3)
P 		66.4
(1.4)
P 		25
C 75.7
(-1.9)
P 		72
(-3)
P 		73.8
(-2.5)
P 		92
(3)
P 		20
avr 68.6
(-0.4) 		59.7
(-3) 		60.5
(-2.8) 		80
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4455
1 2 3 4 5 6 range
A 29.3
(-3)
P 		70.2
(-2.9)
P 		100.7
(-2.9)
P 		314.7
(-2.5)
P 		3079.9
(2.1)
P 		3638
(3)
P 		3609
B 55
(-3)
P 		106.3
(-2.9)
P 		53.7
(-3)
P 		247
(-2.7)
P 		4240.1
(2.7)
P 		4483.9
(3)
P 		4430
C 49.5
(-3)
P 		174.3
(-2.8)
P 		48.6
(-3)
P 		192.2
(-2.8)
P 		3993.4
(3)
P 		3958.5
(2.9)
P 		3945
avr 44.6
(-3) 		116.9
(-2.9) 		67.7
(-3) 		251.3
(-2.7) 		3771.1
(2.6) 		4026.8
(3) 		3982
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172770_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10740
Sequence Type Exemplar sequence
Representative Public ID R13H9.4 NCBI
Target Description R13H9.4 /REP_DB=WormBase Gene ID /WP=CE02806 /GEN=msp-53 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=major sperm protein (MSP-53)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068313(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068313 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-53) (msp-53) mRNA, complete cds. 11/11 A 
SNAP00000029405 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5064668:5065051:-1 11/11 None
GENEFINDER00000029416 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5064668:5065051:-1 11/11 None
R13H9.4 ENSEMBL cdna:known chromosome:CEL140:IV:5064668:5065051:-1 gene:R13H9.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069433 1/11 Cross Hyb Matching Probes A 
NM_062497 3/11 Cross Hyb Matching Probes A 
NM_068370 7/11 Cross Hyb Matching Probes A 
NM_069448 1/11 Cross Hyb Matching Probes A 
NM_068310 6/11 Cross Hyb Matching Probes A 
NM_068312 8/11 Cross Hyb Matching Probes A 
NM_069063 3/11 Cross Hyb Matching Probes A 
NM_062558 2/11 Cross Hyb Matching Probes A 
NM_062569 1/11 Cross Hyb Matching Probes A 
NM_062571 1/11 Cross Hyb Matching Probes A 
NM_068351 7/11 Cross Hyb Matching Probes A 
NM_068354 7/11 Cross Hyb Matching Probes A 
NM_068359 8/11 Cross Hyb Matching Probes A 
NM_068377 8/11 Cross Hyb Matching Probes A 
NM_068379 5/11 Cross Hyb Matching Probes A 
NM_062490 1/11 Cross Hyb Matching Probes A 
NM_062743 1/11 Cross Hyb Matching Probes A 
NM_062748 1/11 Cross Hyb Matching Probes A 
NM_062753 1/11 Cross Hyb Matching Probes A 
NM_069321 1/11 Cross Hyb Matching Probes A 
NM_069339 2/11 Cross Hyb Matching Probes A 
NM_069341 1/11 Cross Hyb Matching Probes A 
NM_069358 2/11 Cross Hyb Matching Probes A 
NM_069359 2/11 Cross Hyb Matching Probes A 
NM_069361 2/11 Cross Hyb Matching Probes A 
NM_069380 2/11 Cross Hyb Matching Probes A 
NM_062457 1/11 Cross Hyb Matching Probes A 
NM_062464 1/11 Cross Hyb Matching Probes A 
NM_068372 5/11 Cross Hyb Matching Probes A 
SNAP00000024816 1/11 Cross Hyb Matching Probes None
GENEFINDER00000024829 1/11 Cross Hyb Matching Probes None
SNAP00000000807 1/11 Cross Hyb Matching Probes None
GENEFINDER00000000821 1/11 Cross Hyb Matching Probes None
SNAP00000016470 1/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 1/11 Cross Hyb Matching Probes None
SNAP00000019951 2/11 Cross Hyb Matching Probes None
GENEFINDER00000019958 2/11 Cross Hyb Matching Probes None
SNAP00000021327 1/11 Cross Hyb Matching Probes None
SNAP00000021329 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021346 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021348 1/11 Cross Hyb Matching Probes None
SNAP00000031245 1/11 Cross Hyb Matching Probes None
GENEFINDER00000031258 1/11 Cross Hyb Matching Probes None
SNAP00000041369 1/11 Cross Hyb Matching Probes None
SNAP00000041374 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041386 1/11 Cross Hyb Matching Probes None
GENEFINDER00000041398 1/11 Cross Hyb Matching Probes None
SNAP00000043869 1/11 Cross Hyb Matching Probes None
SNAP00000043876 3/11 Cross Hyb Matching Probes None
GENEFINDER00000043883 1/11 Cross Hyb Matching Probes None
SNAP00000002639 8/11 Cross Hyb Matching Probes None
SNAP00000002641 5/11 Cross Hyb Matching Probes None
GENEFINDER00000002656 5/11 Cross Hyb Matching Probes None
GENEFINDER00000002661 8/11 Cross Hyb Matching Probes None
SNAP00000004243 2/11 Cross Hyb Matching Probes None
SNAP00000004244 2/11 Cross Hyb Matching Probes None
SNAP00000004246 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004263 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004265 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004273 2/11 Cross Hyb Matching Probes None
SNAP00000005864 1/11 Cross Hyb Matching Probes None
GENEFINDER00000005869 1/11 Cross Hyb Matching Probes None
SNAP00000006004 7/11 Cross Hyb Matching Probes None
SNAP00000006006 5/11 Cross Hyb Matching Probes None
SNAP00000006011 4/11 Cross Hyb Matching Probes None
SNAP00000006013 8/11 Cross Hyb Matching Probes None
SNAP00000006015 5/11 Cross Hyb Matching Probes None
GENEFINDER00000006018 4/11 Cross Hyb Matching Probes None
GENEFINDER00000006020 7/11 Cross Hyb Matching Probes None
GENEFINDER00000006021 8/11 Cross Hyb Matching Probes None
GENEFINDER00000006023 5/11 Cross Hyb Matching Probes None
GENEFINDER00000006024 5/11 Cross Hyb Matching Probes None
SNAP00000007231 6/11 Cross Hyb Matching Probes None
GENEFINDER00000007236 6/11 Cross Hyb Matching Probes None
SNAP00000023386 1/11 Cross Hyb Matching Probes None
GENEFINDER00000023395 1/11 Cross Hyb Matching Probes None
SNAP00000029404 8/11 Cross Hyb Matching Probes None
GENEFINDER00000029413 8/11 Cross Hyb Matching Probes None
SNAP00000029847 3/11 Cross Hyb Matching Probes None
GENEFINDER00000029849 3/11 Cross Hyb Matching Probes None
SNAP00000034129 7/11 Cross Hyb Matching Probes None
SNAP00000034134 7/11 Cross Hyb Matching Probes None
GENEFINDER00000034137 7/11 Cross Hyb Matching Probes None
GENEFINDER00000034140 7/11 Cross Hyb Matching Probes None
SNAP00000040134 1/11 Cross Hyb Matching Probes None
GENEFINDER00000040145 1/11 Cross Hyb Matching Probes None
SNAP00000041533 2/11 Cross Hyb Matching Probes None
GENEFINDER00000041543 2/11 Cross Hyb Matching Probes None
SNAP00000043472 2/11 Cross Hyb Matching Probes None
SNAP00000043475 1/11 Cross Hyb Matching Probes None
GENEFINDER00000043484 2/11 Cross Hyb Matching Probes None
GENEFINDER00000043489 1/11 Cross Hyb Matching Probes None
SNAP00000043994 4/11 Cross Hyb Matching Probes None
GENEFINDER00000044007 4/11 Cross Hyb Matching Probes None
F26G1.7 1/11 Cross Hyb Matching Probes A 
C33F10.9 1/11 Cross Hyb Matching Probes A 
R05F9.3 1/11 Cross Hyb Matching Probes R A 
R05F9.13 3/11 Cross Hyb Matching Probes A 
F59A6.8 2/11 Cross Hyb Matching Probes None
F58A6.8 2/11 Cross Hyb Matching Probes A 
C34F11.6 1/11 Cross Hyb Matching Probes A 
C34F11.4 1/11 Cross Hyb Matching Probes A 
K05F1.2 1/11 Cross Hyb Matching Probes A 
ZK1248.6 1/11 Cross Hyb Matching Probes A 
ZK1248.4 1/11 Cross Hyb Matching Probes A 
C09B9.6 6/11 Cross Hyb Matching Probes A 
R13H9.2 8/11 Cross Hyb Matching Probes A 
Y59E9AR.7 7/11 Cross Hyb Matching Probes A 
Y59E9AR.1 7/11 Cross Hyb Matching Probes A 
F36H12.7 8/11 Cross Hyb Matching Probes A 
F36H12.6 2/11 Cross Hyb Matching Probes None
ZK354.5 7/11 Cross Hyb Matching Probes A 
ZK354.4 5/11 Cross Hyb Matching Probes A 
ZK354.10 4/11 Cross Hyb Matching Probes None
ZK354.11 8/11 Cross Hyb Matching Probes A 
ZK354.1 5/11 Cross Hyb Matching Probes A 
Y59H11AM.1 3/11 Cross Hyb Matching Probes A 
ZK1251.6 1/11 Cross Hyb Matching Probes A 
T13F2.10 2/11 Cross Hyb Matching Probes A 
T13F2.11 1/11 Cross Hyb Matching Probes A 
W08D2.9 1/11 Cross Hyb Matching Probes None
K07F5.1 2/11 Cross Hyb Matching Probes A 
K07F5.2 2/11 Cross Hyb Matching Probes A 
K07F5.3 2/11 Cross Hyb Matching Probes A 
F32B6.6 2/11 Cross Hyb Matching Probes A 
C04G2.3 1/11 Cross Hyb Matching Probes None
C04G2.4 1/11 Cross Hyb Matching Probes A 
K08F4.8 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4787144-4899172(+) 93.23 93.23
chrIV:5260583-5260967(+) 98.96 98.96
chrIV:5249434-5249815(+) 98.18 98.18
chrIV:5062573-5062957(+) 98.18 98.18
chrIV:5297956-5298340(+) 96.35 96.35
chrIV:5316517-5316901(+) 96.35 96.35
chrIV:8571425-8571809(+) 94.79 94.79
chrIV:9894608-9894992(+) 93.75 93.75
chrIV:9770025-9770409(+) 93.49 93.49
chrIV:10093057-10093457(+) 93.75 93.75
chrII:4892294-4892678(-) 94.01 94.01
chrII:4916162-4916546(-) 94.01 94.01
chrIV:5064673-5065057(-) 100.0 100.0
chrIV:5312722-5313106(-) 98.7 98.7
chrIV:5241396-5241777(-) 98.18 98.18
chrIV:5294951-5295335(-) 98.18 98.18
chrIV:5055626-5056010(-) 97.92 97.92
chrIV:9766750-9767134(-) 94.01 94.01
chrIV:9836013-9836397(-) 93.75 93.75
chrIV:10143333-10143717(-) 93.49 93.49
Public Domain and Genome References
Gene Title major sperm protein (MSP-53)
Gene Symbol msp-53
Ensembl R13H9.4
Entrez Gene 177275 Entrez gene
SwissProt P53017 EMBL-EBI
WormBase CE02806 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 1.0E-71
blast CAB03362 Hypothetical protein T13F2.11 [Caenorhabditis elegans] ref|NP_501742.1| Major Sperm Protein family member (msp-78) [Caenorhabditis elegans] sp|Q94053|MSP78_CAEEL Major sperm protein 78 (MSP) 2.0E-71
blast AAK68539 Hypothetical protein Y59H11AM.1 [Caenorhabditis elegans] ref|NP_501464.1| Y59H11AM.1 [Caenorhabditis elegans] 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 4.7E-35
Sequence
Target Sequence 
>C. ELEGANS:172770_X_AT
tctgtcccaccaggagacatccaaacccagccgggtaccaagattgtgttcaatgcccca
tacgacgacaagcacacctaccacatcaaggtgataaactcatcggctcgccgtattgga
tacggtatcaagaccaccaatatgaagagacttggagttgatccaccatgtggagttctc
gacccaaaggaagcagttcttcttgccgtttcctgtgatgctttcgcctttggacaagaa
gacaccaacaacgatcgtatcactgttgagtggaccaacaccccggatggagctgccaag
caattccgccgtgaatggttccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGTCCCACCAGGAGACATCCAAA 26 609 22 Antisense
ACCCAGCCGGGTACCAAGATTGTGT 618 89 46 Antisense
TCAATGCCCCATACGACGACAAGCA 376 633 71 Antisense
TGATAAACTCATCGGCTCGCCGTAT 451 563 113 Antisense
ACCATGTGGAGTTCTCGACCCAAAG 516 83 186 Antisense
AAGGAAGCAGTTCTTCTTGCCGTTT 544 159 208 Antisense
TGATGCTTTCGCCTTTGGACAAGAA 98 565 237 Antisense
GAAGACACCAACAACGATCGTATCA 700 339 259 Antisense
ACTGTTGAGTGGACCAACACCCCGG 488 97 283 Antisense
GGATGGAGCTGCCAAGCAATTCCGC 403 515 306 Antisense
GCAATTCCGCCGTGAATGGTTCCAA 205 317 321 Antisense
Sequence Source Affymetrix Proprietary Database