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[172742_x_at]

Description C08B11.4 /REP_DB=WormBase Gene ID /WP=CE27798 /GEN=nrf-6 /TR=SW:Q09225 /GB=CAA86669.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 91      4grp_highest_8_8 > 26      4grp_high_8_8 > 57      6grp_highest_nonorm > 74      6grp_total > 94      4grp_total > 63      6grp_highest_sub > 05      6grp_cgc > 88      6grp_high1_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 418
1 2 3 4 range
A 728.3
(1.4)
P 		642.4
(-0.1)
P 		817.4
(3)
P 		480.5
(-3)
P 		337
B 809.7
(2.9)
P 		684.3
(-3)
P 		811.2
(3)
P 		690.7
(-2.7)
P 		127
C 796
(1)
P 		640.4
(-2.1)
P 		898.5
(3)
P 		594.2
(-3)
P 		304
avr 778
(1.5) 		655.7
(-1.4) 		842.4
(3) 		588.5
(-3) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 556.5
(-2.3)
P 		507.8
(-3)
P 		643.8
(-1.2)
P 		613.5
(-1.6)
P 		950.6
(3)
P 		552.3
(-2.4)
P 		443
B 544.2
(-3)
P 		655.8
(-1.2)
P 		760.3
(0.6)
P 		655.6
(-1.2)
P 		906.8
(3)
P 		756.9
(0.5)
P 		363
C 434.8
(-3)
P 		626.7
(-0.1)
P 		538.6
(-1.4)
P 		753.5
(1.8)
P 		835.2
(3)
P 		740.1
(1.6)
P 		400
avr 511.8
(-3) 		596.8
(-1.7) 		647.6
(-0.9) 		674.2
(-0.5) 		897.5
(3) 		683.1
(-0.3) 		386
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 265.2
(3)
P 		139.5
(-0.9)
P 		213.7
(1.4)
P 		70.3
(-3)
P 		195
B 258.2
(3)
P 		169
(-1.8)
P 		188
(-0.8)
P 		147.2
(-3)
P 		111
C 263.5
(1.6)
P 		184.1
(-0.6)
P 		313.1
(3)
P 		100.2
(-3)
P 		213
avr 262.3
(3) 		164.2
(-0.8) 		238.3
(2.1) 		105.9
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 266
1 2 3 4 5 6 range
A 102.6
(-3)
P 		180.6
(-0.5)
P 		140.2
(-1.8)
P 		155.6
(-1.3)
P 		286.8
(3)
P 		134.5
(-2)
P 		184
B 189.3
(-2.5)
P 		218.3
(-1.6)
P 		184.6
(-2.7)
P 		173.7
(-3)
P 		368.4
(3)
P 		282.3
(0.3)
P 		195
C 161.2
(-1.9)
P 		191.2
(-0.6)
P 		137.1
(-3)
P 		151.5
(-2.4)
P 		270.3
(3)
P 		247.8
(2)
P 		133
avr 151
(-3) 		196.7
(-1.3) 		154
(-2.9) 		160.3
(-2.6) 		308.5
(3) 		221.5
(-0.3) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172742_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3173
Sequence Type Exemplar sequence
Representative Public ID C08B11.4 NCBI
Target Description C08B11.4 /REP_DB=WormBase Gene ID /WP=CE27798 /GEN=nrf-6 /TR=SW:Q09225 /GB=CAA86669.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063279(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063279 NCBI Caenorhabditis elegans Nose Resistant to Fluoxetine family member (nrf-6) (nrf-6) mRNA, complete cds. 11/11 A 
SNAP00000039305 ENSEMBL cdna:SNAP chromosome:CEL140:II:8028974:8032624:-1 11/11 None
GENEFINDER00000039312 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8028974:8032624:-1 11/11 None
C08B11.4 ENSEMBL cdna:known chromosome:CEL140:II:8028876:8032630:-1 gene:C08B11.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8028970-8032621(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nrf-6
Ensembl C08B11.4
Entrez Gene 174287 Entrez gene
SwissProt Q09225 EMBL-EBI
WormBase CE27798 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1624885_AT dm
DROSGENOME1:144989_AT dm
DROSGENOME1:144988_AT dm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16747 transferase activity, transferring groups other than amino-acyl groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86669 Hypothetical protein C08B11.4 [Caenorhabditis elegans] ref|NP_495680.1| Nose Resistant to Fluoxetine family member (nrf-6) [Caenorhabditis elegans] gb|AAD51972.1| transmembrane protein NRF-6 [Caenorhabditis elegans] sp|Q09225|NRF6_CAEEL Nose resistant to fluoxetine protein 6 precursor (Protein nrf-6) 0.0
blast T19074 hypothetical protein C08B11.4 - Caenorhabditis elegans 0.0
blast CAE67775 Hypothetical protein CBG13350 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495680.1 11 309-331,378-400,423-445,476-498,536-558,565-587,614-636,653-675,685-707,720-742,762-784
C08B11.4 11 309-331,378-400,423-445,476-498,536-558,565-587,614-636,653-675,685-707,720-742,762-784
SNAP00000039305 11 309-331,378-400,423-445,476-498,536-558,565-587,614-636,653-675,685-707,720-742,762-784
GENEFINDER00000039312 11 309-331,378-400,423-445,476-498,536-558,565-587,614-636,653-675,685-707,720-742,762-784
Sequence
Target Sequence 
>C. ELEGANS:172742_X_AT
gttgcgtatcttttgaatgcctggactactcgagagcaaaaagatcttaagataaagctt
gaaagaagaactgtaattatttgttggtgcacttcaacagttcttggattatactctgta
tttggcttgtactggtttgcaaagacgggagatatttctaaaccttgggaggtatataat
catgattttgaatttattagaaacttttttctacagattttgtacaccatttttggaaca
ccagcatatgctttagcattgggatgggttgttttcgcgtgtacaactggaaatggtgga
cctgtagacacaattctctcttggagactatttgttccgttatctaaaattactttttgt
gcatatttgcttcatccaatcatgcttcaaatctacaatttgagtcgaccacagcccttc
cattttaccacattcattcaaatgattcggtacacagtcgaagctgtttttgcttcctac
acaattgcattcttcttttctctag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGCGTATCTTTTGAATGCCTGGA 510 439 1900 Antisense
GAATGCCTGGACTACTCGAGAGCAA 49 331 1914 Antisense
GGTGCACTTCAACAGTTCTTGGATT 367 499 1985 Antisense
ATTGGGATGGGTTGTTTTCGCGTGT 416 3 2157 Antisense
GGACCTGTAGACACAATTCTCTCTT 148 523 2197 Antisense
GGAGACTATTTGTTCCGTTATCTAA 469 517 2222 Antisense
TTTGCTTCATCCAATCATGCTTCAA 581 667 2265 Antisense
TACAATTTGAGTCGACCACAGCCCT 617 641 2293 Antisense
CTTCCATTTTACCACATTCATTCAA 110 219 2316 Antisense
CTGTTTTTGCTTCCTACACAATTGC 216 237 2363 Antisense
ACAATTGCATTCTTCTTTTCTCTAG 164 115 2380 Antisense
Sequence Source Affymetrix Proprietary Database