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[172737_x_at]

Description F42A10.4 /REP_DB=WormBase Gene ID /WP=CE01295 /GEN=efk-1 /TR=SW:O01991 /GB=AAA19075.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 95      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 98      6grp_total > 76      4grp_total > 04      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1283
1 2 3 4 range
A 670.4
(-1.5)
P 		440.1
(-3)
P 		484.2
(-2.7)
P 		1365
(3)
P 		925
B 642.9
(-1.7)
P 		386
(-2.9)
P 		371.2
(-3)
P 		1654.3
(3)
P 		1283
C 635.6
(-1.7)
P 		466.6
(-3)
P 		515.7
(-2.6)
P 		1235.4
(3)
P 		769
avr 649.6
(-1.7) 		430.9
(-3) 		457
(-2.8) 		1418.2
(3) 		987

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1513
1 2 3 4 5 6 range
A 518.2
(-3)
P 		545.1
(-2.9)
P 		661.8
(-2.4)
P 		1300.6
(0.2)
P 		982.9
(-1.1)
P 		1981
(3)
P 		1463
B 468.5
(-3)
P 		567.5
(-2.5)
P 		854.6
(-1.1)
P 		1219.3
(0.7)
P 		994.6
(-0.4)
P 		1692.1
(3)
P 		1224
C 588.7
(-3)
P 		584.6
(-3)
P 		934.3
(-1.2)
P 		1234.9
(0.3)
P 		852.6
(-1.6)
P 		1765.4
(3)
P 		1181
avr 525.1
(-3) 		565.7
(-2.8) 		816.9
(-1.6) 		1251.6
(0.4) 		943.4
(-1.1) 		1812.8
(3) 		1288
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 267
1 2 3 4 range
A 244.1
(3)
P 		95.5
(-3)
P 		126.6
(-1.7)
P 		199.8
(1.2)
P 		149
B 205
(-0.3)
P 		95.3
(-2.8)
P 		86
(-3)
P 		352.5
(3)
P 		267
C 210.4
(3)
P 		134.2
(-3)
P 		179.7
(0.6)
P 		208.3
(2.8)
P 		76
avr 219.8
(1.6) 		108.3
(-3) 		130.8
(-2.1) 		253.5
(3) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 536
1 2 3 4 5 6 range
A 95.5
(-3)
P 		193.9
(-1.5)
P 		144.1
(-2.2)
P 		329.9
(0.6)
P 		296.6
(0.1)
P 		482.3
(3)
P 		387
B 163
(-3)
P 		188.9
(-2.7)
P 		207.5
(-2.4)
P 		322.9
(-1)
P 		404.1
(0.1)
P 		631
(3)
P 		468
C 218.2
(-2.4)
P 		178.4
(-3)
P 		237.8
(-2.1)
P 		248.3
(-2)
P 		275.9
(-1.6)
P 		591
(3)
P 		413
avr 158.9
(-3) 		187.1
(-2.6) 		196.5
(-2.4) 		300.4
(-0.9) 		325.5
(-0.6) 		568.1
(3) 		409
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172737_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7382
Sequence Type Exemplar sequence
Representative Public ID F42A10.4 NCBI
Target Description F42A10.4 /REP_DB=WormBase Gene ID /WP=CE01295 /GEN=efk-1 /TR=SW:O01991 /GB=AAA19075.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027401(11), NM_001027402(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027401 NCBI Caenorhabditis elegans Elongation Factor Kinase family member (efk-1) (efk-1) mRNA, complete cds. 11/11 None
NM_001027402 NCBI Caenorhabditis elegans Elongation Factor Kinase family member (efk-1) (efk-1) mRNA, complete cds. 11/11 None
SNAP00000011646 ENSEMBL cdna:SNAP chromosome:CEL140:III:6165229:6168470:-1 11/11 None
GENEFINDER00000011663 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6165229:6168470:-1 10/11 None
F42A10.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:6164840:6168494:-1 gene:F42A10.4 11/11 None
F42A10.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:6164840:6168487:-1 gene:F42A10.4 11/11 None
F42A10.4b ENSEMBL cdna:known chromosome:CEL140:III:6165229:6168470:-1 gene:F42A10.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6165227-6168469(-) 100.0 100.0
Public Domain and Genome References
Ensembl F42A10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN63383 Elongation factor kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022572.1| Elongation Factor Kinase family member (efk-1) [Caenorhabditis elegans] sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) gb|AAB58268.1| elongation factor-2 kinase EFK-1A isoform [Caenorhabditis elegans] 0.0
blast AAN63384 Elongation factor kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022573.1| Elongation Factor Kinase family member (efk-1) [Caenorhabditis elegans] gb|AAB58269.1| elongation factor-2 kinase EFK-1B isoform [Caenorhabditis elegans] 0.0
blast T30958 hypothetical protein F42A10.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004166 EMBL-EBI MHCK/EF2 kinase domain 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:172737_X_AT
gacgctcgtgtaccaatcaaatacgacaagcagtctgcaattttccatttggatatcgct
cggaagtgtggaatccttgaggctgtgctaacatcggctcatattgttctcggattacca
catgaattgttgaaagaagtcaccgttgatgatctgtttcctaatgggtttggagaacag
gaaaatggaattcgagctgataaaggacaaaaaccttgtgacctagaagagttcggctcc
gatctgatggaaattgctgcagagatgggtgataagggtgcaatgctgtacatggcacac
gcttatgaaactggtcagcatctcggaccgaatcgaagaacggattataagaaatcgatt
gattggtatcaacgcgtcgttggattccaagaagaagaagaacttgactctgattgtgga
aaaacgacattctcctcatttgctccactgactcgtcacgagatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGCTCGTGTACCAATCAAATACG 601 379 1447 Antisense
AGCAGTCTGCAATTTTCCATTTGGA 697 77 1475 Antisense
GAATCCTTGAGGCTGTGCTAACATC 532 329 1517 Antisense
GGCTCATATTGTTCTCGGATTACCA 495 539 1542 Antisense
GTTGATGATCTGTTTCCTAATGGGT 331 437 1591 Antisense
GACCTAGAAGAGTTCGGCTCCGATC 479 383 1666 Antisense
GCAATGCTGTACATGGCACACGCTT 74 323 1726 Antisense
ATGGCACACGCTTATGAAACTGGTC 657 51 1738 Antisense
GAAACTGGTCAGCATCTCGGACCGA 641 357 1753 Antisense
AAAAACGACATTCTCCTCATTTGCT 691 129 1866 Antisense
TGCTCCACTGACTCGTCACGAGATT 271 581 1887 Antisense
Sequence Source Affymetrix Proprietary Database