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[172725_x_at] major sperm protein

Description R05F9.3 /REP_DB=WormBase Gene ID /WP=CE04805 /GEN=msp-32 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster 6grp_total > 99      4grp_total > 04      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 433
1 2 3 4 range
A 18.5
(-3)
A 		18.5
(-3)
A 		25.3
(-2.9)
A 		363.8
(3)
P 		345
B 135.1
(2)
A 		31.3
(-2.5)
A 		18.7
(-3)
A 		157.2
(3)
A 		139
C 92.1
(-1.9)
A 		10.7
(-3)
A 		14.4
(-2.9)
A 		443.3
(3)
P 		433
avr 81.9
(-1.8) 		20.2
(-3) 		19.5
(-3) 		321.4
(3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1924
1 2 3 4 5 6 range
A 22.9
(-3)
A 		50
(-2.9)
A 		165.6
(-2.4)
A 		370.9
(-1.5)
M 		940.9
(0.9)
P 		1419.8
(3)
P 		1397
B 31.5
(-2.9)
A 		79.6
(-2.7)
A 		13.9
(-3)
A 		210.2
(-2)
P 		1058.2
(2.3)
P 		1191.4
(3)
P 		1178
C 13.4
(-3)
A 		229.1
(-2.3)
A 		17.4
(-3)
A 		272.8
(-2.2)
A 		1937.1
(3)
P 		1268.3
(0.9)
P 		1924
avr 22.6
(-3) 		119.6
(-2.5) 		65.6
(-2.8) 		284.6
(-1.8) 		1312.1
(3) 		1293.2
(2.9) 		1290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 6.8
(-2.7)
A 		4
(-3)
A 		6.6
(-2.7)
A 		53.2
(3)
P 		49
B 43.1
(3)
A 		7.7
(-2.5)
A 		4.3
(-3)
A 		33.5
(1.5)
A 		39
C 30.5
(-0.7)
A 		3.1
(-3)
A 		5
(-2.8)
A 		74.7
(3)
P 		72
avr 26.8
(-0.3) 		4.9
(-3) 		5.3
(-3) 		53.8
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 623
1 2 3 4 5 6 range
A 4.2
(-3)
A 		17.8
(-2.8)
A 		36.1
(-2.4)
A 		94.1
(-1.4)
M 		283.9
(1.9)
P 		345.7
(3)
P 		342
B 11
(-2.9)
A 		26.5
(-2.7)
A 		3.4
(-3)
A 		55.7
(-2.3)
P 		429.9
(2.8)
P 		444.3
(3)
P 		441
C 5
(-3)
A 		69.9
(-2.4)
A 		4.4
(-3)
A 		54.9
(-2.5)
A 		626.8
(3)
P 		424.6
(1.1)
P 		622
avr 6.7
(-3) 		38.1
(-2.6) 		14.6
(-2.9) 		68.2
(-2.2) 		446.9
(3) 		404.9
(2.4) 		440
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172725_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5713
Sequence Type Exemplar sequence
Representative Public ID R05F9.3 NCBI
Target Description R05F9.3 /REP_DB=WormBase Gene ID /WP=CE04805 /GEN=msp-32 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062490(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062490 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-32) (msp-32) mRNA, complete cds. 11/11 None
SNAP00000043869 ENSEMBL cdna:SNAP chromosome:CEL140:II:4898602:4899174:1 11/11 None
GENEFINDER00000043883 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4898602:4899174:1 11/11 None
R05F9.3 ENSEMBL cdna:known chromosome:CEL140:II:4898602:4899174:1 gene:R05F9.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062487 1/11 Cross Hyb Matching Probes A 
NM_062497 3/11 Cross Hyb Matching Probes A 
NM_068313 1/11 Cross Hyb Matching Probes None
NM_062558 3/11 Cross Hyb Matching Probes A 
NM_062559 1/11 Cross Hyb Matching Probes None
NM_062569 2/11 Cross Hyb Matching Probes A 
NM_062505 2/11 Cross Hyb Matching Probes A 
NM_062500 2/11 Cross Hyb Matching Probes A 
NM_062811 1/11 Cross Hyb Matching Probes None
NM_069321 1/11 Cross Hyb Matching Probes A 
NM_069359 1/11 Cross Hyb Matching Probes A 
NM_062457 2/11 Cross Hyb Matching Probes A 
NM_062464 3/11 Cross Hyb Matching Probes A 
NM_068372 1/11 Cross Hyb Matching Probes None
SNAP00000000807 2/11 Cross Hyb Matching Probes None
SNAP00000000808 1/11 Cross Hyb Matching Probes None
GENEFINDER00000000821 2/11 Cross Hyb Matching Probes None
SNAP00000007649 3/11 Cross Hyb Matching Probes None
GENEFINDER00000007652 3/11 Cross Hyb Matching Probes None
SNAP00000016470 3/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 3/11 Cross Hyb Matching Probes None
SNAP00000019951 3/11 Cross Hyb Matching Probes None
GENEFINDER00000019958 3/11 Cross Hyb Matching Probes None
GENEFINDER00000019959 1/11 Cross Hyb Matching Probes None
SNAP00000021327 2/11 Cross Hyb Matching Probes None
GENEFINDER00000021348 2/11 Cross Hyb Matching Probes None
GENEFINDER00000034379 1/11 Cross Hyb Matching Probes None
SNAP00000034943 2/11 Cross Hyb Matching Probes None
SNAP00000034948 2/11 Cross Hyb Matching Probes None
GENEFINDER00000034961 2/11 Cross Hyb Matching Probes None
GENEFINDER00000034965 2/11 Cross Hyb Matching Probes None
SNAP00000043868 1/11 Cross Hyb Matching Probes None
SNAP00000043876 3/11 Cross Hyb Matching Probes None
GENEFINDER00000043888 1/11 Cross Hyb Matching Probes None
GENEFINDER00000025846 1/11 Cross Hyb Matching Probes None
SNAP00000004244 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004265 1/11 Cross Hyb Matching Probes None
SNAP00000023386 1/11 Cross Hyb Matching Probes None
GENEFINDER00000023395 1/11 Cross Hyb Matching Probes None
F26G1.7 2/11 Cross Hyb Matching Probes A 
F26G1.8 1/11 Cross Hyb Matching Probes None
C33F10.9 3/11 Cross Hyb Matching Probes A 
R05F9.8 1/11 Cross Hyb Matching Probes A 
R05F9.13 3/11 Cross Hyb Matching Probes A 
ZK546.6 2/11 Cross Hyb Matching Probes A 
ZK546.3 2/11 Cross Hyb Matching Probes A 
F59A6.8 3/11 Cross Hyb Matching Probes None
F58A6.8 3/11 Cross Hyb Matching Probes A 
C34F11.6 2/11 Cross Hyb Matching Probes A 
H12I13.1 1/11 Cross Hyb Matching Probes None
ZK1251.6 1/11 Cross Hyb Matching Probes A 
K07F5.2 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4898599-4899172(+) 65.1 100.0
Public Domain and Genome References
Gene Title major sperm protein
Gene Symbol msp-32
Ensembl R05F9.3
Entrez Gene 191709 Entrez gene
SwissProt P53018 EMBL-EBI
WormBase CE04805 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494891 Major Sperm Protein family member (msp-32) [Caenorhabditis elegans] gb|AAA83168.1| Major sperm protein protein 32 [Caenorhabditis elegans] sp|P53018|MSP32_CAEEL Major sperm protein 32 (MSP) 1.0E-111
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 6.0E-66
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 6.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 5.0E-35
Sequence
Target Sequence 
>C. ELEGANS:172725_X_AT
tggtttttggagcgcggcatatgccgaaatgccgatttcaaaattcacggcatatgccga
aaatgccgttttgccgaaaagaaaaatggacggcatatcggcatatgccgatatgccgaa
aaaaatttttggcggcatatgccgttatgccgttatgccgaaaaattccgaatgccgcac
aggtatgatccagacccagccgggtacgaagatcgtcttcaatgcaccatacgacgacaa
gcacacctaccacatcaaggtgattaactcctcggctcgccgtattggatacggtatcaa
gaccaccaatatgaagagacttggagttgatccaccatgtggagtgctcgatccaaagga
agctgttcttctggcagtttcctgcgatgccttcgcttttggacaggaggacactaacaa
cgatcgtatcactgttgagtggaccaacaccccggatggtgctgctaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTTTTTGGAGCGCGGCATATGCC 667 549 42 Antisense
ATGCCGAAAATGCCGTTTTGCCGAA 103 37 95 Antisense
TGCCGTTATGCCGTTATGCCGAAAA 220 585 181 Antisense
TGCCGCACAGGTATGATCCAGACCC 446 585 214 Antisense
GACCCAGCCGGGTACGAAGATCGTC 581 381 234 Antisense
GAAGATCGTCTTCAATGCACCATAC 122 339 249 Antisense
CACCATACGACGACAAGCACACCTA 646 201 266 Antisense
ACTCCTCGGCTCGCCGTATTGGATA 99 99 308 Antisense
GGAAGCTGTTCTTCTGGCAGTTTCC 7 531 399 Antisense
CGATGCCTTCGCTTTTGGACAGGAG 694 247 426 Antisense
CAACACCCCGGATGGTGCTGCTAAG 393 185 486 Antisense
Sequence Source Affymetrix Proprietary Database