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[172724_x_at] major sperm protein

Description R05F9.8 /REP_DB=WormBase Gene ID /WP=CE04811 /GEN=msp-33 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster 6grp_total > 98      4grp_total > 06      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 70.2
(-1.1)
A 		80.2
(-0.7)
A 		23.4
(-3)
A 		169.8
(3)
P 		146
B 112.6
(2.3)
M 		24.2
(-3)
A 		124.8
(3)
A 		64.2
(-0.6)
A 		101
C 102.6
(0.1)
A 		18.8
(-3)
A 		21.1
(-2.9)
A 		182.7
(3)
P 		164
avr 95.1
(0.3) 		41.1
(-3) 		56.4
(-2.1) 		138.9
(3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5186
1 2 3 4 5 6 range
A 19.5
(-3)
A 		41.4
(-3)
A 		143.4
(-2.9)
P 		477.7
(-2.5)
P 		3254.9
(0.7)
P 		5197.9
(3)
P 		5178
B 46.6
(-3)
A 		62.7
(-3)
A 		82.2
(-3)
A 		411.3
(-2.5)
P 		4121.7
(2.7)
P 		4373.8
(3)
P 		4327
C 12
(-3)
A 		163
(-2.8)
P 		119.6
(-2.9)
A 		425.5
(-2.5)
P 		5166.4
(3)
P 		4986
(2.8)
P 		5154
avr 26
(-3) 		89
(-2.9) 		115.1
(-2.9) 		438.2
(-2.5) 		4181
(2.2) 		4852.6
(3) 		4827
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 25.6
(3)
A 		17.4
(0.5)
A 		6.1
(-3)
A 		24.9
(2.8)
P 		20
B 35.9
(3)
M 		6
(-3)
A 		28.9
(1.6)
A 		13.7
(-1.5)
A 		30
C 34
(3)
A 		5.4
(-3)
A 		7.3
(-2.6)
A 		30.8
(2.3)
P 		29
avr 31.8
(3) 		9.6
(-3) 		14.1
(-1.8) 		23.1
(0.6) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1671
1 2 3 4 5 6 range
A 3.6
(-3)
A 		14.7
(-2.9)
A 		31.2
(-2.9)
P 		121.1
(-2.4)
P 		982.2
(1.7)
P 		1265.5
(3)
P 		1262
B 16.2
(-3)
A 		20.9
(-3)
A 		20
(-3)
A 		108.9
(-2.7)
P 		1674.6
(3)
P 		1631
(2.8)
P 		1658
C 4.5
(-3)
A 		49.7
(-2.8)
P 		30.4
(-2.9)
A 		85.6
(-2.7)
P 		1671.8
(3)
P 		1669.1
(3)
P 		1667
avr 8.1
(-3) 		28.4
(-2.9) 		27.2
(-2.9) 		105.2
(-2.6) 		1442.9
(2.7) 		1521.9
(3) 		1514
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172724_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5342
Sequence Type Exemplar sequence
Representative Public ID R05F9.8 NCBI
Target Description R05F9.8 /REP_DB=WormBase Gene ID /WP=CE04811 /GEN=msp-33 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062487(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062487 NCBI Caenorhabditis elegans Major Sperm Protein family member (msp-33) (msp-33) mRNA, complete cds. 11/11 None
SNAP00000043868 ENSEMBL cdna:SNAP chromosome:CEL140:II:4892297:4892680:-1 11/11 None
GENEFINDER00000043888 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4892297:4892680:-1 11/11 None
R05F9.8 ENSEMBL cdna:known chromosome:CEL140:II:4892087:4892680:-1 gene:R05F9.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062497 3/11 Cross Hyb Matching Probes A 
NM_062559 1/11 Cross Hyb Matching Probes A 
NM_062571 1/11 Cross Hyb Matching Probes A 
NM_062505 5/11 Cross Hyb Matching Probes A 
NM_068359 2/11 Cross Hyb Matching Probes A 
NM_062490 2/11 Cross Hyb Matching Probes A 
NM_062500 7/11 Cross Hyb Matching Probes A 
NM_062457 1/11 Cross Hyb Matching Probes A 
NM_062464 2/11 Cross Hyb Matching Probes A 
SNAP00000000807 1/11 Cross Hyb Matching Probes None
SNAP00000000808 1/11 Cross Hyb Matching Probes None
GENEFINDER00000000821 1/11 Cross Hyb Matching Probes None
GENEFINDER00000000824 1/11 Cross Hyb Matching Probes None
SNAP00000007649 2/11 Cross Hyb Matching Probes None
GENEFINDER00000007652 2/11 Cross Hyb Matching Probes None
SNAP00000016470 2/11 Cross Hyb Matching Probes None
GENEFINDER00000016484 2/11 Cross Hyb Matching Probes None
SNAP00000019952 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019959 1/11 Cross Hyb Matching Probes None
SNAP00000021329 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021346 1/11 Cross Hyb Matching Probes None
SNAP00000034943 7/11 Cross Hyb Matching Probes None
SNAP00000034948 5/11 Cross Hyb Matching Probes None
GENEFINDER00000034961 7/11 Cross Hyb Matching Probes None
GENEFINDER00000034965 5/11 Cross Hyb Matching Probes None
SNAP00000043869 2/11 Cross Hyb Matching Probes None
SNAP00000043876 3/11 Cross Hyb Matching Probes None
GENEFINDER00000043883 2/11 Cross Hyb Matching Probes None
SNAP00000002639 2/11 Cross Hyb Matching Probes None
SNAP00000002641 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002661 2/11 Cross Hyb Matching Probes None
F26G1.7 1/11 Cross Hyb Matching Probes A 
F26G1.8 1/11 Cross Hyb Matching Probes None
C33F10.9 2/11 Cross Hyb Matching Probes A 
R05F9.3 2/11 Cross Hyb Matching Probes R A 
R05F9.13 3/11 Cross Hyb Matching Probes A 
ZK546.6 7/11 Cross Hyb Matching Probes A 
ZK546.3 5/11 Cross Hyb Matching Probes A 
F59A6.8 2/11 Cross Hyb Matching Probes None
F58A6.9 1/11 Cross Hyb Matching Probes A 
C34F11.4 1/11 Cross Hyb Matching Probes A 
F36H12.7 2/11 Cross Hyb Matching Probes A 
F36H12.6 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4933009-4933393(+) 97.4 97.4
chrII:4939884-4940265(+) 96.35 96.35
chrII:4787144-4899172(+) 95.83 95.83
chrII:4825399-4825783(+) 94.27 94.27
chrII:5194343-5194727(+) 93.23 93.23
chrII:5199774-5200158(+) 92.97 92.97
chrIV:5260583-5260967(+) 94.27 94.27
chrIV:5062573-5062957(+) 93.75 93.75
chrIV:5297956-5298340(+) 93.75 93.75
chrIV:5316517-5316901(+) 93.75 93.75
chrIV:5249434-5249815(+) 92.71 92.71
chrII:4892294-4892678(-) 100.0 100.0
chrII:4916162-4916546(-) 95.83 95.83
chrII:5012855-5013236(-) 92.97 92.97
chrIV:5064673-5065057(-) 94.01 94.01
chrIV:5294951-5295335(-) 94.01 94.01
chrIV:5312722-5313106(-) 93.23 93.23
chrIV:5241396-5241777(-) 92.71 92.71
chrIV:5055626-5056010(-) 92.97 92.97
chrIV:9766750-9767134(-) 92.97 92.97
Public Domain and Genome References
Gene Title major sperm protein
Gene Symbol msp-33
Ensembl R05F9.8
Entrez Gene 173844 Entrez gene
SwissProt P53019 EMBL-EBI
WormBase CE04811 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494888 Major Sperm Protein family member (msp-33) [Caenorhabditis elegans] gb|AAA83165.1| Major sperm protein protein 33 [Caenorhabditis elegans] sp|P53019|MSP33_CAEEL Major sperm protein 33 (MSP) 9.0E-72
blast AAA85761 Major sperm protein protein 50 [Caenorhabditis elegans] emb|CAA94282.1| Hypothetical protein K07F5.1 [Caenorhabditis elegans] gb|AAK85493.1| Major sperm protein protein 53 [Caenorhabditis elegans] gb|AAB42254.1| Major sperm protein protein 51 [Caenorhabditis elegans] gb|AAB42255.1| Major sperm protein protein 113 [Caenorhabditis elegans] gb|AAB42253.1| Major sperm protein protein 59 [Caenorhabditis elegans] gb|AAB42256.1| Major sperm protein protein 65 [Caenorhabditis elegans] gb|AAC71087.1| Major sperm protein protein 64 [Caenorhabditis elegans] gb|AAA68711.1| Major sperm protein protein 142 [Caenorhabditis elegans] gb|AAA96204.1| Major sperm protein protein 45 [Caenorhabditis elegans] gb|AAC26926.1| Major sperm protein protein 19 [Caenorhabditis elegans] ref|NP_500773.1| Major Sperm Protein family member (msp-113) [Caenorhabditis elegans] ref|NP_500771.1| Major Sperm Protein family member (msp-51) [Caenorhabditis elegans] ref|NP_501759.1| Major Sperm Protein family member (msp-81) [Caenorhabditis elegans] ref|NP_500780.1| Major Sperm Protein family member (msp-65) [Caenorhabditis elegans] ref|NP_500778.1| Major Sperm Protein family member (msp-59) [Caenorhabditis elegans] ref|NP_500760.1| Major Sperm Protein family member (msp-19) [Caenorhabditis elegans] ref|NP_500714.1| Major Sperm Protein family member (msp-53) [Caenorhabditis elegans] ref|NP_495149.1| Major Sperm Protein family member (msp-64) [Caenorhabditis elegans] ref|NP_495144.1| Major Sperm Protein family member (msp-142) [Caenorhabditis elegans] ref|NP_494972.1| Major Sperm Protein family member (msp-50) [Caenorhabditis elegans] ref|NP_494959.1| Major Sperm Protein family member (msp-45) [Caenorhabditis elegans] ref|NP_494898.1| Major Sperm Protein family member (msp-31) [Caenorhabditis elegans] ref|NP_494865.1| Major Sperm Protein family member (msp-40) [Caenorhabditis elegans] gb|AAK31477.1| Major sperm protein protein 40 [Caenorhabditis elegans] gb|AAA83175.1| Major sperm protein protein 31 [Caenorhabditis elegans] sp|P53017|MSP19_CAEEL Major sperm protein 19/31/40/45/50/51/53/59/61/65/81/113/142 (MSP) 4.0E-71
blast CAB03362 Hypothetical protein T13F2.11 [Caenorhabditis elegans] ref|NP_501742.1| Major Sperm Protein family member (msp-78) [Caenorhabditis elegans] sp|Q94053|MSP78_CAEEL Major sperm protein 78 (MSP) 6.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.0E-35
Sequence
Target Sequence 
>C. ELEGANS:172724_X_AT
tccgtcccaccaggagatatccagacccagccgggtacgaagatcgtgttcaatgccccg
tacgacgacaagcacacctaccacatcaaggtgatcaactcatctgctcgccgtattgga
tacggtatcaagaccaccaatatgaagagacttggagttgatccaccatgtggagttctg
gacccaaaggaagctgtttttctggcagtttcctgcgatgccttcgcctttggacaggag
gacactaacaacgatcgtatcactgttgagtggaccaacactccggatggtgctgctaag
caattccgtcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGTCCCACCAGGAGATATCCAGA 355 595 22 Antisense
ATATCCAGACCCAGCCGGGTACGAA 632 17 38 Antisense
ACGAAGATCGTGTTCAATGCCCCGT 697 91 58 Antisense
CAAGGTGATCAACTCATCTGCTCGC 509 183 108 Antisense
TCATCTGCTCGCCGTATTGGATACG 183 621 121 Antisense
CCACCATGTGGAGTTCTGGACCCAA 386 271 184 Antisense
GAAGCTGTTTTTCTGGCAGTTTCCT 328 343 211 Antisense
ATGCCTTCGCCTTTGGACAGGAGGA 478 41 239 Antisense
GGAGGACACTAACAACGATCGTATC 215 513 258 Antisense
ACCAACACTCCGGATGGTGCTGCTA 292 85 295 Antisense
TGGTGCTGCTAAGCAATTCCGTCGG 313 553 309 Antisense
Sequence Source Affymetrix Proprietary Database